Induction of tumor cytotoxic immune cells using a protein from the bitter melon (Momordica charantia)

Cunnick, Joan E.; Sakamoto, Kazuko; Chapes, Stephen K.; Fortner, William; Takemoto, Dolores J.

doi:10.1016/0008-8749(90)90321-h

Cited by 44 publications

(27 citation statements)

References 12 publications

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“…have cytotoxic activity on canceorous cells. Their phytochemical has the ability to inhibit an enzyme named guanylate cyclise which involves in cancerous growth and in leukemia [15] A chemical analog of their protein named MAP-30 inhibit prostate tumor growth by induced cell apoptosis.…”

Section: Bioactive Compound and Their Mode Of Actionmentioning

confidence: 99%

Anticancerous Potential of Medicinal Plants- A Review

Shourie¹

2017

WJPR

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Section: Bioactive Compound and Their Mode Of Actionmentioning

confidence: 99%

Anticancerous Potential of Medicinal Plants- A Review

Shourie¹

2017

WJPR

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“…Clinical trials have found much evidence that M. charantia can improve immune cell function in patients with cancer (Cunnick et al, 1990;Yuan et al, 1999). In this work, the cytotoxic activity of methanol leaf extract of M. charantia on cancer cell lines as well as its effects on the normal cell in vitro was examined.…”

Section: Introductionmentioning

confidence: 99%

Anti-proliferative and cytotoxic effects of methanol extract of the leaves of Momordica charantia L. (Cucurbitaceae) on vascular smooth muscle cells (VSMC) and HT-29 cell lines

Ofuegbe¹,

Oyagbemi²,

Omóbòwálé³

et al. 2017

J. Med. Plants Res.

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Momordica charantia has been used traditionally for the treatment of several ailments. Some natural plant products are known to exhibit cytotoxic or anti-proliferative effects on cancer cell lines, such plants offer a promising therapeutic approach as an anti-tumor agent. In this study, the anti-proliferative effects of graded concentrations of methanol leaf extract of M. charantia (MEMC) at different point time was examined on vascular smooth muscle cells (VSMC), and human colorectal adenocarcinoma cell lines (HT-29) were investigated using the MTT proliferation assay. The result showed that after 24 and 48 h, the effect of MEMC on the VSMC alone and in the presence of the mitogens was more of proliferation. In the case of HT 29 cytotoxic study, the extract at all doses used caused a cytotoxic effect. The effect of the extract of M. charantia was more pronounced and consistent at 72 h time point exhibiting cytotoxic actions against cancer cell lines, the extract showed no toxic action to normal cells. This suggests a possible use of the plant M. charantia to identify compounds of possible interest in the treatment of cancer. While the extract possesses proliferative effects on the VSMCs, the reverse is the case, where it exhibited cell inhibitory effects on HT 29 cell lines indicating that the plant exhibit cytotoxic effects and could then serve as lead agents in the search for anticancer drugs from natural products.

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“…Traditionally, it is used as a medicinal food [1]. The fruit of the plant is reported to possess antidiabetic [2][3][4], anti-cancer, cytotoxic [5][6][7], cholesterol-lowering [8][9][10], antioxidant [11], anti-ulcer [12], analgesic, anti-inflammatory [13,14], anti-fertility [15], and abortifacient [16] properties.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of high-performance thin-layer chromatography method for determination of vicine in herbal extract and formulation

Bedi¹,

Gauttam²,

Kalia³

2014

Acta Chromatographica

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Summary.A simple, selective, precise, and reproducible high-performance thin-layer chromatography (HPTLC) method for the analysis of vicine in herbal extracts and formulations containing Momordica charantia was developed. The study was performed on aluminum-based pre-coated TLC plates (silica gel 60F 254 ). The chromatograms of samples were developed in twin trough glass chamber pre-saturated with mobile phase comprised of ethyl acetate-methanol-water-formic acid (7.5:3:1:0.1, v/v/v/v) at room temperature (25 ± 2°C). The densitometric analysis was carried out in absorbance mode at 278 nm. The optimized mobile phase showed a compact spot of vicine (R F = 0.31 ± 0.02) from the other constituents present in the samples. The linear regression analysis data for the calibration plots showed good linearity (r 2 = 0.9990) with respect to peak area in the range of 200-1400 ng spot −1 . The method was validated as per International Conference on Harmonization (ICH) guidelines. The limits of detection and quantification (50 and 150 ng spot −1 , respectively) were also established. The proposed method has shown the excellent recovery (98.01-99.33%), which supports the suitability of the method for the analysis. Statistical analysis of the obtained data showed the selectivity of the proposed method for vicine estimation in the herbal samples.

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Induction of tumor cytotoxic immune cells using a protein from the bitter melon (Momordica charantia)

Cited by 44 publications

References 12 publications

Anticancerous Potential of Medicinal Plants- A Review

Anticancerous Potential of Medicinal Plants- A Review

Anti-proliferative and cytotoxic effects of methanol extract of the leaves of Momordica charantia L. (Cucurbitaceae) on vascular smooth muscle cells (VSMC) and HT-29 cell lines

Development and validation of high-performance thin-layer chromatography method for determination of vicine in herbal extract and formulation

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