Induction of traps by<i>Ostertagia ostertagi</i>larvae, chlamydospore production and growth rate in the nematode-trapping fungus<i>Duddingtonia flagrans</i>

Grønvold, J.; Nansen, P.; Henriksen, S. A.; Larsen, Mona; Wolstrup, J.; Bresciani, J.; Rawat, H. S.; Fribert, L.

doi:10.1017/s0022149x00015571

Cited by 70 publications

(47 citation statements)

References 22 publications

(19 reference statements)

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“…Their conclusion shows that even both species are predators they were not stimulated by nematodes. Gronvold et al (1996) observed trap formation of Duddingtonia flagrans being influenced by hyphae aging, with better activity at 30 o C than 20 o C, enduring for two or three weeks, period from what on trap inductility reduced. The authors also guarantee that larvae migration (Ostertagia ostertagi) was indispensable for trap formation and as their movement reduces at 10 o C it explicates low trap formation at this temperature, though their work did not considered control plates to conclude whether trap would be induced only in plates with nematodes.…”

Section: Resultsmentioning

confidence: 97%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

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Biological control is a method of controlling pests through the use of other living organisms. The purposes of this study were to test Hohenbuehelia species as biological control agents against Panagrellus redivivus in vitro, evaluating nematodes influence on mycelia growth; establishing daily indexes for predation and growth and setting predation percentage. Five species previously identified as 436-Hohenbuehelia mastrucata (Nematoctonus hamatus), 528-H. bullulifera (not described so far), 581-H. paraguayensis (N. sp.), 582-H. sp. (N. sp.) and 631-H. portegna (N. campylosporus) were submitted to anamorphic purification directly from basidioma. Afterwards, 100 nematodes were added to each pure colony for predation test. Evaluation started right after 24 hours of nematode-fungus interaction. Immobilized and/or penetrated nematodes were counted and mycelia growth was measured. Results were subjected to variance analyses. Hohenbuehelia mastrucata had the best performance in growth speed, followed by H. portegna and H. paraguayensis; Nematodes multiplyied much but none specie grew more as an influence of their movement under mycelium, however all species formed trap devices and some of them produced adhesive or repelent substances. Trap devices were formed in control plates also. The plates of H. paraguayensis without nematodes grew more than treatments. Cumulative predation of H. portegna was the highest at 24 (195.5%) and 48 hours (235%). At the last evaluation day, H. paraguayensis preyed the same amount (185.75%) than H. portegna, followed by H. mastrucata (109.51%). Resulst of predation daily indexes displayed chronological activity for each isolate, where H. portegna was very reactive at first 24 hours, H. mastrucata raised its predacious activity in 48 hours being constant from this time on and H. paraguayensis pointed out itself at 72 hours. Other species presented low predation and growth indexes throughout experiment.

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Section: Resultsmentioning

confidence: 97%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

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“…This was particularly the case for the faecal deposits of May, July and September with a reduction in larval numbers from 50 to 93% compared to the control. These periods of time probably correspond to the temperature requirements of D. flagrans for hyphal growth rate and trap production which are between 15 and 30 °C [9,11]. However, for the May deposit, no larvae were recovered until 8 weeks after faecal deposition in the control plot, when a sharp increase occurred with a rather low percentage of development of the eggs into L3 (0.018 to 0.028 L3/eggs deposited according to the nematode species).…”

Section: Discussionmentioning

confidence: 93%

“…Such observations have been made by Mounport et al [17] in the South of France in Mediterranean garrigues where faeces represented the main reservoir of infective larvae during the dry periods of the grazing season. This delay in migration for several weeks could lead to a reduction in the production of the nematode trapping structures by the fungus, that has been reported to occur after 2 or 3 weeks exposure to such conditions in the laboratory [11]. In addition, reduced fungal growth due to the low humidity might further explain the moderate (50-60%) efficacy of D. flagrans at that time.…”

Section: Discussionmentioning

confidence: 97%

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Effect of the nematophagous fungus, Duddingtonia flagrans, on the larval development of goat parasitic nematodes: a plot study

Chartier

Pors

2003

Vet. Res.

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-Effective alternatives to anthelmintic treatment against nematode parasites of goats are required because of the high prevalence of benzimidazole resistance. Towards this objective, the nematophagous fungus, Duddingtonia flagrans (Df), was used in a plot study against two main parasitic nematode species of goats, Teladorsagia circumcincta (Tcir) and Trichostrongylus colubriformis (Tcol). Worm-free, culled goats were experimentally infected with strains of Tcir and Tcol to constitute donors. Half of the animals were periodically given Df chlamydospores at a daily dose of 2.5 × 10 5 spores/kg BW while the remaining animals were kept as controls. At 5 time periods i.e. March, May, July, September and November 2001, corresponding to the main grazing season in France for goats, faeces were collected from the 6th day of fungus administration for the following 2 days to obtain approximately 1 kg of faeces from each group of animals: Tcir/Control, Tcol/Control, Tcir/Fungus, Tcol/Fungus. For each period and each group, the faeces were deposited on a 1 m 2 grass plot and the grass was cut (3 replicates) on weeks 2, 4, 6, 8 ,12 after deposition, for infective larval recovery. Larvae were counted and the results were expressed as a ratio of larvae/ eggs deposited. On the plots with the control faeces deposited in March, July and September, the grass infectivity due to Tcir and Tcol was similar and the maximum number occurred between 2 and 4 weeks post deposition. In May, the maximum numbers of larvae were not recorded until 8 weeks after deposition, due to high daily temperatures and dryness. In November, larval development took place only for Tcir. On the plots with the fungus treated faeces, a significant reduction in grass infectivity occurred for both nematodes and ranged from 50-60% in May, July and November deposits to 80-90% in the September deposit. On the contrary to these findings, no difference was recorded between the fungus and control plots for the March deposit. In conclusion, D. flagrans is suitable for reducing the number of infective larvae in the herbage during the main part of the grazing period for the most important digestive nematodes of goats.goat / biological control / Duddingtonia flagrans / nematode parasite

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“…Estudos utilizando-se da microscopia eletrônica de varredura para documentar o modo de ação destes fungos nematófagos sobre nematóides fitoparasitos (BARRON, 1977;DROWSET e REID, 1977;JANSSON e NORDBRING-HERTZ, 1988;LOPES-LORCA e DUNCAN, 1991; MAIA e SANTOS, 1995; MAIA e SANTOS, 1996; MAIA e SANTOS, 1997;MAIA e SANTOS, 1999;MAIA, 2000) e sobre helmintos de animais domésticos (PADILHA e GIVES, 1996;GRONVOLD et al, 1996;GRAMINHA, 2000), têm sido realizados por poucos pesquisadores.…”

Section: Introductionunclassified

Microscopia eletrônica de varredura da habilidade predatória de agentes do biocontrole de nematóides

Silveira¹,

Graminha²,

Nunes³

et al. 2001

Sem. Ci. Agr.

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Resumo: Fungos nematófagos têm sido estudados como uma alternativa promissora para o manejo de nematóides de plantas e parasitos gastrintestinais de bovinos. Resultados encorajadores têm sido obtidos com algumas espécies, por vários pesquisadores. A partir de amostras de solo coletadas em diferentes culturas e agroecossistemas provenientes de diversas localidades do Brasil, foram isolados, pelo método de espalhamento de solo, os fungos nematófagos Arthrobotrys javanica, Dactylella leptospora, Monacrosporium eudermatum, M. robustum e Nematoctonus campylosporus, no Centro de Pesquisas em Sanidade Animal (CPPAR) da UNESP/FCAV, Câmpus de Jaboticabal -SP, e a habilidade predatória destes fungos sobre o nematóide de vida livre Panagrellus sp. foi estudada no Laboratório de Microscopia Eletrônica de Varredura. Este trabalho teve como objetivo documentar ao microscópio eletrônico de varredura (MEV) estruturas morfológicas desses fungos tais como: estruturas de captura, tamanho, forma e septação dos conídios, grampos de conexão em N. campylosporus, assim como os nematóides de vida livre capturados por estes fungos. Palavras-chave: controle biológico, fungos nematófogos, microscopia eletrônica de varredura.Abstract: Nematode-trapping fungi have been studied as a promising alternative to control bovine gastrointestinal parasites and plant parasitic nematodes. Some encouraging results have been achieved with some species, by many researchers. Nematophagous fungi such as Arthrobotrys javanica, Dactylella leptospora, Monacrosporium eudermatum, M. robustum e Nematoctonus campylosporus were isolated from soil samples collected from different crops and agronomic environments proceeding from varies places in Brazil, making use of soil spreading methods. These fungi species were isolated at the Center of Animal Parasitology (CPPAR) at UNESP/FCAV, Campus Jaboticabal -SP. The predatory ability of these fungi, on free living nematodes (Panagrellus sp.) was documented at the Scanning Electron Microscopy Lab. The objectives of this work were to document, through Scanning Electron Microscopy (SEM), the morphological structures of these fungi, such as: capturing structures, size, form, septation of the conidia, connection clamp, exclusively on N. campylosporus, as well as the free-living nematodes captured by these fungi.

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Induction of traps byOstertagia ostertagilarvae, chlamydospore production and growth rate in the nematode-trapping fungusDuddingtonia flagrans

Cited by 70 publications

References 22 publications

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Effect of the nematophagous fungus, Duddingtonia flagrans, on the larval development of goat parasitic nematodes: a plot study

Microscopia eletrônica de varredura da habilidade predatória de agentes do biocontrole de nematóides

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