Induction of Transplantation Tolerance in Adult Rats by Vascularized Spleen Transplantation

Abstract

It was demonstrated by Bitter-Seurmann et al. that tolerance to subsequent skin allografts can be brought about, in some rat strain combinations, by heterotopic spleen allografts. We report here that in adult rats, tolerance can be also induced by vascularized spleen allografts using various strain combinations. DA (RT1av1) rats always accepted major histocompatible complex (MHC)-compatible but minor histocompatibility antigen (miH)-incompatible ACI (RT1av1) spleen grafts spontaneously, and further that those DA rats bearing ACI spleen grafts accepted subsequent ACI skin grafts indefinitely. In contrast, Fischer 344 (RT1lvl) rats always accepted not only the "weak" MHC-incompatible Lewis (RT1l) spleen grafts but also subsequent Lewis skin grafts indefinitely, when recipients were administered a short course of FK506 only at the time of primary spleen transplantation. Furthermore, in a number of MHC- and miH-incompatible strain combinations, recipients always accepted primary spleen grafts indefinitely when given a short course of FK506, but those recipients bearing donor spleens revealed split tolerance, that is, rejected subsequent donor-specific skin grafts but accepted heart grafts indefinitely. We conclude that the spleen seems to be a possible organ to induce transplantation tolerance, and that even if the MHC and miH are mismatched, unresponsiveness to at least heart grafts can be achieved by the administration of additional FK506 at the time of spleen transplantation.

Th1/Th2 Cytokine Profile of the Immune Response in Limb Component Transplantation

“…6,35,36 Murray proposed a scale of antigenicity with skin and lung being the most antigenic tissues, liver, and heart being less antigenic, and kidney and pancreas as the least antigenic. 37 Suzuki et al described a hierarchy of the ability of tissues or organ to facilitate induction and maintain transplantation tolerance that corresponds with their specific degrees of antigenicity.…”

Engraftment of Syngeneic and Allogeneic Endothelial Cells, Hepatocytes and Cholangiocytes Into Partially Hepatectomized Rats Previously Treated With Mitomycin C

“…To determine if treatment with anti-CD3 would allow allogeneic transplantation, colony formation was assessed following transplantation of ACI liver isolates (RT1av1 haplotype) into F344 DPPIV− host rats (RT1lv1 haplotype) (34) treated with MMC, anti-CD3 and PH. These two rat strains that differ at major histocompatibility loci have been used extensively in previous studies of the immune mechanisms responsible for allograft rejection (35–37).…”

Laparoscopic hand-assisted spleen autotransplantation

“…Spleen inclusion was first seen as a possible solution to prevent early graft thrombosis and to induce transplantation tolerance [21]. Spleen allografts were used in rats to produce unresponsiveness to the transplantation of pancreatic islet cells [7].…”