Induction of the NFκ-B signal transduction pathway in response to Corynebacterium diphtheriae infection

Ott, Lisa; Scholz, Brigitte; Höller, Martina; Hasselt, Kristin; Ensser, Armin; Burkovski, Andreas

doi:10.1099/mic.0.061879-0

Cited by 11 publications

(17 citation statements)

References 37 publications

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“…Compared with adhesion rates found for different C. diphtheriae strains (for review, see Ott & Burkovski, 2014) and invasion rates determined (e.g. Ott et al, 2013), C. ulcerans rates observed here were at least two-to fivefold higher, indicating the high infectious potential of this pathogen. This is further supported by detrimental effects on epithelial cells as shown by the breakdown of transepithelial resistance of cell monolayers after infection with C. ulcerans BR-AD22 and 809.…”

Section: Discussionmentioning

confidence: 94%

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

et al. 2015

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Corynebacterium ulcerans is an emerging pathogen transmitted by a zoonotic pathway to humans. Despite rising numbers of infections and potentially fatal outcomes, data on the colonization of the human host are lacking up to now. In this study, adhesion of two C. ulcerans isolates to human epithelial cells, invasion of host cells and the function of two putative virulence factors with respect to these processes were investigated. C. ulcerans strains BR-AD22 and 809 were able to adhere to Detroit562 and HeLa cells, and invade these epithelial cell lines with a rate comparable to other pathogens as shown by scanning electron microscopy, fluorescence microscopy and replication assays. Infection led to detrimental effects on the cells as deduced from measurements of transepithelial resistance. Mutant strains of putative virulence factors phospholipase D and DIP0733 homologue CULC22_00609 generated in this study showed no influence on colonization under the experimental conditions tested. The data presented here indicate a high infectious potential of this emerging pathogen.

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Section: Discussionmentioning

confidence: 94%

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

et al. 2015

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“…Strain DSM43989 is a PW8 strain used for toxoid production. In previous studies, DSM43989 showed low adhesion to and invasion into epithelial cells in comparison to other non-toxigenic isolates [21] and we were interested to elucidate the reason for this behavior. The number and types of surface pili are important for adhesion and invasion [19,23,24]; however, their presence has been verified by atomic force microscopy [19,23].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Corynebacterium diphtheriae macrophage interaction: Dispensability of corynomycolic acids for inhibition of phagolysosome maturation and identification of a new gene involved in synthesis of the corynomycolic acid layer

et al. 2017

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Corynebacterium diphtheriae is the causative agent of diphtheria, a toxin mediated disease of upper respiratory tract, which can be fatal. As a member of the CMNR group, C. diphtheriae is closely related to members of the genera Mycobacterium, Nocardia and Rhodococcus. Almost all members of these genera comprise an outer membrane layer of mycolic acids, which is assumed to influence host-pathogen interactions. In this study, three different C. diphtheriae strains were investigated in respect to their interaction with phagocytic murine and human cells and the invertebrate infection model Caenorhabditis elegans. Our results indicate that C. diphtheriae is able to delay phagolysosome maturation after internalization in murine and human cell lines. This effect is independent of the presence of mycolic acids, as one of the strains lacked corynomycolates. In addition, analyses of NF-κB induction revealed a mycolate-independent mechanism and hint to detrimental effects of the different strains tested on the phagocytic cells. Bioinformatics analyses carried out to elucidate the reason for the lack of mycolates in one of the strains led to the identification of a new gene involved in mycomembrane formation in C. diphtheriae.

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“…The ability of Cor. diphtheriae to enter cultured respiratory epithelial cells was first shown by Hirata et al (2002) and validated by a number of further studies (Mattos-Guaraldi, 2002;Bertuccini et al, 2004;Ott et al, 2010aOtt et al, , b, 2013.…”

Section: Introductionmentioning

confidence: 99%

Characterization of DIP0733, a multi-functional virulence factor of Corynebacterium diphtheriae

et al. 2015

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Corynebacterium diphtheriae is typically recognized as an extracellular pathogen. However, a number of studies revealed its ability to invade epithelial cells, indicating a more complex pathogen-host interaction. The molecular mechanisms controlling and facilitating internalization of Cor. diphtheriae are poorly understood. In this study, we investigated the role of DIP0733 as virulence factor to elucidate how it contributes to the process of pathogen-host cell interaction. Based on in vitro experiments, it was suggested recently that the DIP0733 protein might be involved in adhesion, invasion of epithelial cells and induction of apoptosis. A corresponding Cor. diphtheriae mutant strain generated in this study was attenuated in its ability to colonize and kill the host in a Caenorhabditis elegans infection model system. Furthermore, the mutant showed an altered adhesion pattern and a drastically reduced ability to adhere and invade epithelial cells. Subsequent experiments showed an influence of DIP0733 on binding of Cor. diphtheriae to extracellular matrix proteins such as collagen and fibronectin. Furthermore, based on its fibrinogen-binding activity, DIP0733 may play a role in avoiding recognition of Cor. diphtheriae by the immune system. In summary, our findings support the idea that DIP0733 is a multi-functional virulence factor of Cor. diphtheriae.

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Induction of the NFκ-B signal transduction pathway in response to Corynebacterium diphtheriae infection

Cited by 11 publications

References 37 publications

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

Colonization of human epithelial cell lines by Corynebacterium ulcerans from human and animal sources

Analysis of Corynebacterium diphtheriae macrophage interaction: Dispensability of corynomycolic acids for inhibition of phagolysosome maturation and identification of a new gene involved in synthesis of the corynomycolic acid layer

Characterization of DIP0733, a multi-functional virulence factor of Corynebacterium diphtheriae

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