Induction of the formation of a coli-bacterio-phage and colicin by hydroperoxide

Šmarda, Jan; Čermák, Vojtěch

doi:10.1007/bf02148229

Cited by 8 publications

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“…Microcins can be either chromosomally or plasmid encoded, whereas colicins have been found only on plasmids (13,29,30). Colicin production is usually correlated with an SOS response to stress (22,34), and release of the colicin typically occurs through cell lysis. Microcins are secreted from intact cells (8,27).…”

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confidence: 99%

Characterization of a Novel Microcin That Kills Enterohemorrhagic Escherichia coli O157:H7 and O26

Eberhart

Deringer

Brayton

et al. 2012

Appl Environ Microbiol

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ABSTRACTA novel phenotype was recently identified in which specific strains ofEscherichia coliinhibit competingE. colistrains via a mechanism that was designated “proximity-dependent inhibition” (PDI). PDI-expressing (PDI+)E. coliis known to inhibit susceptible (PDI−)E. colistrains, including several enterohemorrhagic (EHEC) and enterotoxigenic (ETEC)E. colistrains. In this study, every strain from a genetically diverse panel ofE. coliO157:H7 (n= 25) and additional strains ofE. coliserovar O26 were susceptible to the PDI phenotype. LIVE/DEAD staining was consistent with inhibition by killing of susceptible cells. Comparative genome analysis identified the genetic component of PDI, which is composed of a plasmid-borne (Incl1) operon encoding a putative microcin and associated genes for transport, immunity, and microcin activation. Transfer of the plasmid to a PDI−strain resulted in transfer of the phenotype, and deletion of the genes within the operon resulted in loss of the inhibition phenotype. Deletion of chromosomally encodedtolCalso resulted in loss of the inhibitory phenotype, and this confirmed that the putative microcin is most likely secreted via a type I secretion pathway. Deletion of an unrelated plasmid gene did not affect the PDI phenotype. Quantitative reverse transcription (RT)-PCR demonstrated that microcin expression is correlated with logarithmic-phase growth. The ability to inhibit a diversity ofE. colistrains indicates that this microcin may influence gut community composition and could be useful for control of important enteric pathogens.

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confidence: 99%

Characterization of a Novel Microcin That Kills Enterohemorrhagic Escherichia coli O157:H7 and O26

Eberhart

Deringer

Brayton

et al. 2012

Appl Environ Microbiol

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“…If then, when dealing with this same prophage and under the same physiological conditions, UV-rays raised the spontaneous production of phage 133 times (SMARDA 1960), hydrogen peroxide 35 times (SMARDA 1962) and broth, irradiated by UV-rays, 4 times, it means that respectively 133, 35 and 4 times a higher number of cells reacted to induction compared with the number of cells producing phage spontaneously. And this can be explained in accordance with the above findings only then, when we accept the two following conclusions: 1) Inductive agents have no direct effect upon prophage or the colicinogenic factor, but interfere primarily with the cell, this means, with its metabolism (most probably with the metabolism of nucleic acids) ; with this reaction, secondary effect upon the prophage occurs.…”

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confidence: 99%

Induction of the synthesis of phage and colicin in a culture medium irradiated by UV‐rays

Šmarda

Čermák

1964

J of Basic Microbiology

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It is possible to increase considerably the spontaneous production of the bacteriophage in many lysogenic cultures by induction; this induction has so far been successfully obtained by many physical and chemical factors. Of the physical agents the most used is UV-radiation a t a wave-length of about 2537 A, absorbedselectively by DNA, andionising radiation: X-rays a t a wave-length of 0.9 A and gamma-rays a t an average energy of photons 600 keV and further, alpha particlesand deuterons. Similar effect wasalso obtained bythermo-shocks (CAVALLO 1953).Of the chemical substances used as inducing agents the best known are hydrogen peroxide and some other organic peroxides (terbutylperoxide and butadiendioxide), furthermore epoxides, ethyleneimines, 1-azaserine, mustard gas, and especially nitrogen mustard: So far unique is the account of induction by ribonuclease (E'IETRZYKOWSKA 1960). Sulfhydril compounds (reduced glutathione, thiomalic acid, thioglycolic acid) bring about induction

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Induction of the synthesis of phage and colicin in a culture medium irradiated by UV-rays

Šmarda

Čermák

1964

Z Allg Mikrobiol

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Induction of the formation of a coli-bacterio-phage and colicin by hydroperoxide

Cited by 8 publications

References 5 publications

Characterization of a Novel Microcin That Kills Enterohemorrhagic Escherichia coli O157:H7 and O26

Characterization of a Novel Microcin That Kills Enterohemorrhagic Escherichia coli O157:H7 and O26

Induction of the synthesis of phage and colicin in a culture medium irradiated by UV‐rays

Induction of the synthesis of phage and colicin in a culture medium irradiated by UV-rays

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