Induction of the blood-brain barrier-related enzyme alkaline phosphatase in cerebral capillary endothelial cells by neuroectodermal cells in vitro

Beuckmann, Carsten T.; Gath, U.; Rauh, J.; Heitkamp, B.; Galla, Hans‐Joachim

doi:10.1016/0928-4931(94)90026-4

Cited by 4 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand it has already been demonstrated that BCEC can be re-induced to display ALP activity without being inhibited in their proliferation. This effect has been observed in mixed cocultures of BCEC with epithelial cells of the choroid plexus, which also belong to the glial cell family (Beuckmann et al, 1994).…”

Section: Resultsmentioning

confidence: 75%

Untitled

et al. 1997

View full text Add to dashboard Cite

Astrocytes, a member of the glial cell family in the central nervous system, are assumed to play a crucial role in the formation of the blood-brain barrier (BBB) in vertebrates. It was shown that astrocytes induce BBB-properties in brain capillary endothelial cells (BCEC) in vitro. We now established an astroglial cell line of non-tumoral origin. The cloned cell line (A7) shows a highly increased proliferation rate and expresses the astrocytic marker glial fibrillary acidic protein. Furthermore, the clone A7 expresses S-100-protein and vimentin, which are also expressed by primary cultured astrocytes. This cell line therefore shows general astrocytic features. In addition, we were able to show that A7 cells re-induce the BBB-related marker enzyme alkaline phosphatase in BCEC, when these two cell types are co-cultured. Thus we have a cell line which can be readily cultured in large quantities, shows common astrocyte properties and is able to influence BCEC with respect to a BBB-related feature.

show abstract

Section: Resultsmentioning

confidence: 75%

Untitled

et al. 1997

View full text Add to dashboard Cite

show abstract

“…γ-GT and ALP, the characteristic enzymes of the BBB, play an important role in the active transport, facilitated diffusion and barrier function of BMECs [31][32][33]. In addition, the selective barrier function of the BBB is also closely related to the tight junction (TJ) formed between the endothelial cells.…”

Section: Selection Of Signature Proteinsmentioning

confidence: 99%

Biomanufacturing of a novel in vitro biomimetic blood-brain barrier model

Liu

Zhang³

et al. 2020

Biofabrication

View full text Add to dashboard Cite

A glioma is a malignant tumor that severely threatens human health. However, it is difficult for most therapeutic agents to penetrate through the blood-brain barrier (BBB) and exhibit their antineoplastic activity in the brain. In this article, a biomimetic in vitro BBB model was created by a composite process, this model can provide a significant foundation for the research of drug transport, tumor treatment, tumor microenvironment and other fields. A series of tests and comparative experiments were performed to evaluate this model. The tests showed that the model enabled preliminary simulation of the structure and function of the BBB. Experimental results demonstrated: (1) the new technology enabled controlled release of growth factors and successfully induced endothelial progenitor cells into endothelial cells. Compared with the traditional gold standard, the Transwell model, the expression of four specific proteins that are related to the BBB characteristics was significantly increased (alkaline phosphatase(ALP) by 89.82%, γ-GT by 88.86%, zonula occludens-1 (ZO-1) by 57.40%, and Claudin-5 by 102.32%) in this model; (2) astrocytes had a promoting effect on the microvascular endothelial cells to form tight junction (ZO-1 increased by 249.35%, Claudin-5 increased by 184.99%), and there was a great difference between whether these two types of cells were contact cultured or not; (3) the gelatinous cell U118 had a destructive effect on the tight junction of BBB (ZO-1 decreased by 55.86%, Claudin-5 decreased by 37.84%).

show abstract

Induction of the Blood/Brain‐Barrier‐Associated Enzyme Alkaline Phosphatase in Endothelial Cells from Cerebral Capillaries is Mediated Via cAMP

Beuckmann

Hellwig

Galla

1995

European Journal of Biochemistry

View full text Add to dashboard Cite

The mammalian bloodhain barrier is located at the endothelial cells of the cerebral capillaries. Alkaline phosphatase is associated to a very large extent with these cells and has been established as a marker enzyme for a differentiated bloodhain barrier phenotype in vivo and in vitro. Nevertheless cultured brain capillary endothelial cells (BCEC) lose this marker enzyme because of a cessation of de novo synthesis. Since astrocytes have been shown to possess the capability to re-induce the enzymic activity of alkaline phosphatase in BCEC in vitro we were interested in the second messengers involved in the signal-transduction mechanism of this induction in BCEC. For this reason we treated cultured porcine BCEC with a water-soluble and membrane-permeable analogue of CAMP, 8-(4-~hlorophenylthio)-cAMP (CIPhS-CAMP) in the absence of astrocytes. By means of enzymic activity assays we were able to show that within three days the activity of alkaline phosphatase increased up to sixfold compared with the controls. The total activity of alkaline phosphatase in ClPhS-CAMP-treated BCEC was comparable to that of freshly isolated cells. Addition of cycloheximide inhibited the alkaline phosphatase activity increase. We conclude that cAMP is one of the second messengers involved in the induction of alkaline phosphatase activity in BCEC in vitro.

show abstract

Induction of the blood-brain barrier-related enzyme alkaline phosphatase in cerebral capillary endothelial cells by neuroectodermal cells in vitro

Cited by 4 publications

References 15 publications

Untitled

Untitled

Biomanufacturing of a novel in vitro biomimetic blood-brain barrier model

Induction of the Blood/Brain‐Barrier‐Associated Enzyme Alkaline Phosphatase in Endothelial Cells from Cerebral Capillaries is Mediated Via cAMP

Contact Info

Product

Resources

About