Induction of the action potential mechanism in slow muscle fibres of the frog

Miledi, Ricardo; Stefani, E.; Steinbach, Alan

doi:10.1113/jphysiol.1971.sp009597

Cited by 72 publications

(44 citation statements)

References 22 publications

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“…4A). Although the nerve stump of the iliofibularis muscle is slightly longer than that of the pyriformis muscle, action potentials were likely to be present in both muscles at this time (Miledi et al 1971;Schalow & Schmidt, 1977). After 1 month in culture three slow fibres of the iliofibularis investigated were capable of producing 'overshooting' action potentials (see Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Thus 2 weeks after nerve transaction in vivo all the slow fibres are capable of producing fully developed action potentials (Miledi et al 1971;Epstein & Bekerman, 1976;Schalow & Schmidt, 1977 and see Fig. 3A).…”

Section: Resultsmentioning

confidence: 99%

“…Following denervation or reinnervation by fast axons, slow muscle fibres acquire the ability to produce action potentials (Miledi, Stefani & Steinbach, 1971). The appearance of the action potential is sensitive to blockers of RNA synthesis (Schmidt & Tong, 1973) and has a time course which is dependent on the length of the nerve stump left after transaction (Epstein & Bekerman, 1976;Schalow & Schmidt, 1977).…”

Section: Introductionmentioning

confidence: 99%

“…Previously, slow fibres kept in organ culture for several weeks showed only small regenerative responses (see Miledi et al 1971), while, under similar culture conditions, the fast fibres develop a high level of extrajunctional acetylcholine sensitivity characteristic of denervated fibres (Harris & Miledi, 1968 …”

Section: Introductionmentioning

confidence: 99%

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Induction of action potentials in cultured slow muscle fibres of the frog.

Cull-Candy¹,

Miledi²,

Uchitel³

1980

The Journal of Physiology

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SUMMARY1. Slow muscle fibres of the frog were maintained in a simple organ culture system for up to 28 days at 18 0C. Slow fibres cultured for one week resembled innervated fibres in their lack of ability to generate an action potential.2. By 10 days a few fibres had acquired the action potential mechanism and in muscles cultured for 28 days all slow fibres examined were capable of generating action potentials most of which reached or exceeded 0 mV membrane potential.3. Slow muscle fibres which were denervated for two weeks in vivo retained their ability to generate overshooting action potentials after 4 weeks of culture. Thus, in the culture system described, slow muscle fibres are capable of developing action potentials and the action potential mechanism in these fibres remains for at least one month in culture.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Induction of action potentials in cultured slow muscle fibres of the frog.

Cull-Candy¹,

Miledi²,

Uchitel³

1980

The Journal of Physiology

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“…In this regard the ability of denervated frog slow muscle fibres to generate action potentials (Kuffler & Vaughan-Williams, 1953;Burke & Ginsborg, 1956;Miledi, Stefani & Steinbach, 1971) is also blocked by protein synthesis inhibitors (Schmidt & Tong, 1973 …”

Section: Discussionmentioning

confidence: 99%

Ionic and metabolic dependence of axotomy‐induced somatic membrane changes in crayfish.

Kuwada¹

1981

The Journal of Physiology

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SUMMARY1. Axotomy induces a transient change in the soma membrane from non-spiking to spiking in many neurones of the crayfish abdominal C.N.s. The ionic and metabolic dependence of this phenomenon was investigated in one identified neurone.2. The inward current of axotomy-induced soma spikes is carried primarily by Na ions.3. Addition of 50mM-tetraethylammonium to the external saline unmasks predominantly Ca-mediated soma spikes in control cells. The calcium spikes are not affected by axotomy.4. Axotomy induces a transient increase in delayed rectification of the soma membrane.5. Membrane potential is dependent on the external potassium concentration as predicted by the Nernst function while external sodium has essentially no effect. This is not changed following axotomy.6. The inward current of the axon spike is primarily carried by sodium ions both before and after axotomy. The overshoot of the axon spike is not dramatically changed between the time when axotomy-induced soma spikes are present and when they have disappeared.7. Following axotomy, both the onset and offset of axotomy-induced soma spikes occur earlier when the temperature is raised by 100C.8. Cycloheximide retards or prevents soma spikes normally seen following axotomy.9. The results indicate that axotomy induces a transient increase in voltagedependent Na and K conductances but not Ca conductance, and that these changes are dependent on axotomy-initiated protein synthesis.

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Myoneural junctions in the extraocular muscles of the mouse

1974

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The very fast extraocular muscles have both singly and multiply innervated fibers. The former are among the fastest vertebrate muscle fibers on record, whereas the latter are very slow. In this study single and multiple myoneural junctions were first identified in whole eye muscles of the mouse after staining for cholinesterase. Each group was then embedded separately for electron microscopy. Approximately 10% of the fibers were found to be multiply innervated. The multiple endings and the fibers they innervate resemble those of the tonic bundles of the frog. The majority single endplate is very large in surface view, has sparse junctional folds, and innervates a fiber very rich in mitochondria. These observations were contrary to some frequently held views regarding neuromuscular junctions of very fast fibers. A minority single endplate is smaller, has more junctional folds and innervates a large fiber with fewer and smaller mitochondria.

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Induction of the action potential mechanism in slow muscle fibres of the frog

Cited by 72 publications

References 22 publications

Induction of action potentials in cultured slow muscle fibres of the frog.

Induction of action potentials in cultured slow muscle fibres of the frog.

Ionic and metabolic dependence of axotomy‐induced somatic membrane changes in crayfish.

Myoneural junctions in the extraocular muscles of the mouse

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