Induction of the Accumulation of Tryptamine and Phenols by Endogenous Elicitors in Cell Suspension Cultures of Periwinkle

Garnier, Frédérique; Garnier, Christiane; Garnier, Genevieve; Garnier, Said; Garnier, J.; Garnier, Marc

doi:10.1016/s0176-1617(96)80371-3

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…The specific and total yields of ajmalicine remained constant around 0.75 mg/gDW and 4 mg/L, respectively (data not shown). This result is consistent with the observations of Garnier et al (1996), who did not observe any effect of pectinase addition on ajmalicine synthesis by C. roseus cell suspension cultures. Serpentine levels were slightly lower than that of ajmalicine (data not shown).…”

Section: Resultssupporting

confidence: 93%

Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures

1998

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Late exponential phase hairy root cultures of Catharanthus roseus were elicited with pectinase and jasmonic acid. The effects of elicitor concentration and exposure time on growth and levels of several compounds in the indole alkaloid biosynthetic pathway were monitored. Pectinase decreased the fresh weight to dry weight ratio of the roots, while addition of jasmonic acid had no significant effect. Selective effects on indole alkaloid yields were observed upon addition of elicitors. An increase of 150% in tabersonine specific yield was observed upon addition of 72 units of pectinase. Transient studies at the same level demonstrated possible catabolism as serpentine, tabersonine, and lochnericine levels decreased immediately after elicitation. The levels of these compounds recovered back to control levels or were higher than the control levels after some time. Jasmonic acid was found to be a unique elicitor leading to an enhancement in flux to several branches in the indole alkaloid pathway. Jasmonic acid addition caused an increase in the specific yields of ajmalicine (80%), serpentine (60%), lochnericine (150%), and hörhammericine (500%) in dosage studies. Tabersonine, the likely precursor of lochnericine and hörhammericine, decreased at lower levels of jasmonic acid and then increased with increasing jasmonic acid concentration. Transient studies showed that lochnericine and tabersonine levels go through a maxima, then decrease back to control levels and reduce below control levels, respectively. The yields of ajmalicine, serpentine, and hörhammericine increased continuously after the addition of jasmonic acid. The methods described in this article could generally be used in devising strategies for enhancement in productivity of secondary metabolites and for probing and studying the complex secondary metabolite pathways in plant tissue cultures.

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Section: Resultssupporting

confidence: 93%

Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures

1998

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“…The other members of the 4.1 family (ezrin, moesin, radixin) share their strongest homologies in the 30 kD NH z" terminal region. The predicted secondary structure is derived from the algorithms of Garnier et al (157).…”

Section: Ankyrinmentioning

confidence: 99%

Of Membrane Stability and Mosaics: The Spectrin Cytoskeleton

Morrow

Rimm

Kennedy

et al. 1997

Comprehensive Physiology

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The sections in this article are: The Red Cell Membrane Skeleton How Does the Spectrin Membrane Skeleton Stabilize the Red Cell? The Trilayer Couple—Spectrin as A Membrane Organizer Components of the Erythrocyte Membrane Skeleton Spectrin Actin Ankyrin Protein 4.1 Adducin Dematin (Protein 4.9) Pallidin (Protein 4.2) p55 (an Erythrocyte Membrane‐Associated Guanylate Kinase) Stomatin Tropomyosin and Tropomodulin Dynamin Interactions with Phospholipids The Spectrin Skeleton of Non‐Erythroid Cells Spatial and Temporal Polarization Proteins Interacting with Spectrin in Non‐Erythroid Cells Cytoskeletal Elements Adhesion Proteins Evolving Concepts Conclusions: The Linked Mosaic Model

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“…The production of phytoalexin was induced in suspension cultures of carrot by biotic elicitors ( Aspergillus niger ( A. niger ) and Fusarium moniliforme ( F. moniliforme )) and by the abiotic elicitor (19). Garnier and co‐workers (20) have reported that suspension cultures of periwinkle treated with either A. niger and Rhizopus or Trichoderma viride ( T. viride ) increased the intracellular accumulation of tryptamine, but ajmalicine contents did not increase. Recently, a two‐stage process for enhanced ajmalicine production by C. roseus cell cultures elicited by Aspergillus niger was developed in a 20 L airlift bioreactor (21).…”

Section: Introductionmentioning

confidence: 99%

“…Suspension cultures elicited with T. viride for 48 h showed a 3-fold increase (87 µg g -1 DW) in ajmalicine contents, whereas A. niger and F. moniliforme synthesized a 2-fold increase in alkaloid and yielded 52 and 56 µg g -1 DW ajmalicine, respectively. C. roseus cells of different age (5,10,15,20, and 25 days old) were treated with a 5% elicitor of A. niger, F. moniliforme, and T. viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 µg g -1 DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T. viride.…”

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confidence: 99%

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

Namdeo¹,

Patil

Fulzele

2002

Biotechnology Progress

119

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Suspension cultures of Catharanthus roseus (C. roseus) were elicited with fungal cell wall fragments of Aspergillus niger (A. niger), Fusarium moniliforme (F. moniliforme), and Trichoderma viride (T. viride). The effects of elicitor dosage, exposures time, and age of subculture on ajmalicine accumulation were studied. A higher concentration of elicitor extract responded positively to C. roseus suspension cultures. Ajmalicine accumulation increased by about 3-fold when cells were treated with A. niger, F.moniliforme, and T. viride. The maximum ajmalicine production (75 microg g(-1) dry weight (DW)) was observed in cells treated with T. viride. Cell cultures were elicited with 5% preparation of A. niger, F. moniliforme, and T. viride and exposed for 24, 48, 72, and 96 h. for elicitation. Suspension cultures elicited with T. viride for 48 h showed a 3-fold increase (87 microg g(-1) DW) in ajmalicine contents, whereas A. niger and F. moniliforme synthesized a 2-fold increase in alkaloid and yielded 52 and 56 microg g(-1) DW ajmalicine, respectively. C. roseus cells of different age (5,10, 15, 20, and 25 days old) were treated with a 5% elicitor of A. niger, F. moniliforme, and T. viride and investigated elicitors activity at different age of cell cultures. Maximum yield 166 microg g(-1) DW of ajmalicine was synthesized in 20 day old suspension cultures treated with T. viride. A longer period of incubation of cell cultures with elicitors adversely affected the ajmalicine synthesis.

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Induction of the Accumulation of Tryptamine and Phenols by Endogenous Elicitors in Cell Suspension Cultures of Periwinkle

Cited by 10 publications

References 30 publications

Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures

Effect of Elicitor Dosage and Exposure Time on Biosynthesis of Indole Alkaloids by Catharanthus roseus Hairy Root Cultures

Of Membrane Stability and Mosaics: The Spectrin Cytoskeleton

Influence of Fungal Elicitors on Production of Ajmalicine by Cell Cultures of Catharanthus roseus

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