Induction of T-lymphocyte responses to a small molecular weight antigen. III. T-T cell interactions to determinants linked together: suppression vs. enhancement.

Abstract

The experiments presented in this paper demonstrate the existence of T-T cell interactions in responses to azobenzenearsonate (ABA)-protein conjugates, and also make the point that the spectrum of T-cell regulation from facilitation (i.e., help) at one end to suppression at the other, which has been well documented in T-B cell interactions, is also followed in T-cell regulation of other T lymphocytes. The data extend the activity of ABA-specific suppressor cells, which were shown to specifically suppress the development of delayed hypersensitivity to ABA-T, to T cells responsible for delayed hypersensitivity to protein antigens provided immunization is carried out with ABA conjugates of these antigens. Thus, suppressor T cells acting on the development of delayed hypersensitivity are not limited in their effects to T cells bearing the same specificity but can effectively suppress responses on immunologically unrelated T cells if they are specific for carrier antigens covalently linked to the ABA-T determinant. Moreover, these studies demonstrate that, as is true of T-B cell interactions, the most efficient T-T cell interactions occur to determinants linked together on the same molecule thus supporting the concept that development of effector T-cell function may involve participation of at least two distinct precursor cells, each of which may convey independent determinant specificities and/or genetic control.

Induction of T-lymphocyte responses to a small molecular weight antigen. II. specific tolerance induced in azebenzenearsonate (ABA)-specific T cells in Guniea pigs by administration of low doses of an ABA conjugate of chloroacetyl tyrosine in incomplete Freund's adjuvant.

“…Indeed, the passive transfer of lymph node and spleen cells from guinea pig donors pretreated with ABA-T in IFA significantly suppressed the subsequent responses of unprimed syngeneic recipients of such cells to sensitization with ABA-T in CFA. Moreover, in the accompanying manuscript, we will present evidence demonstrating that animals rendered unresponsive by ABA-T in IFA possess ABA-specific suppressor cells which are capable of suppressing delayed sensitivity responses to determinants on proteins covalently linked to ABA (29) . These findings, although demonstrating the participation of active suppressor cells in this model, do not define the class of cell responsible for the suppression.…”

ABA‐T Determinant Regulation of Delayed Hypersensitivity

“…This proposal of T-T cell helper effect is also supported by the finding that ABA-T specific helper cells can enhance the anti-HGG DH response to ABA-HGG in guinea pigs (Bullock et al 1975c). This proposal of T-T cell helper effect is also supported by the finding that ABA-T specific helper cells can enhance the anti-HGG DH response to ABA-HGG in guinea pigs (Bullock et al 1975c).…”

Coexistence of immunogenic and suppressogenic epitopes in tumor cells and various types of macromolecules

“…It has been assumed that suppressor cells activated by the overstimulated immune system combine with the helper/inducer cells or the effector cells via an 'antigenic bridge' [9,56], thus enabling effective delivery of a 'suppressive factor' to them. This factor restrains the activity of the target cells.…”

“…When such treated animals were pretreated with injections of ABA-tyrosine (ABA-T) incorporated into incomplete Freund's adjuvant (IFA) the skin reaction to HGG was abrogated. However, ABA-T failed to suppress the HGG skin reaction response in guinea pigs that received injections of HGG and ABA-bovine serum albumin (BSA) [9]. This was interpreted as meaning that suppressor T cells induced with ABA-T combine with the ABA component of A B A -H G G conjugate, whereas the DTH T cells (riD) combine with the HGG part of the conjugate (linked recognition).…”