Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope

Cheung, Y.K.; Cheng, Samuel Chak-Sum; Sin, Fion Wan-Yee; Chan, Kin-Tak; Xie, Yong

doi:10.1016/j.vaccine.2007.05.025

Cited by 47 publications

(58 citation statements)

References 36 publications

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“…Studies to date have used either H-2K b class I SCTs or HLA-A2 class I SCTs in the form of DNA vaccines to test the efficacy of SCTs in priming CD8 ϩ T cell responses in vivo in mouse models (5)(6)(7)(8)(9). In this study, we describe the properties of an SCT containing the H-2D b H chain and presenting a well-characterized epitope derived from influenza virus nucleoprotein (NP), residues 366 -374.…”

Section: T Here Is Continued Interest In Improving the Methods Availamentioning

confidence: 99%

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A Single-Chain H-2Db Molecule Presenting an Influenza Virus Nucleoprotein Epitope Shows Enhanced Ability at Stimulating CD8+ T Cell Responses In Vivo

Palmowski

Parker

Choudhuri

et al. 2009

The Journal of Immunology

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We have generated a construct encoding a single-chain H-2Db mouse MHC class I molecule in which an influenza virus nucleoprotein (NP) epitope, amino acid sequence ASNENMDAM, is fused to mouse β2-microglobulin and the Db H chain via flexible linker sequences. This single-chain trimer (SCT) was efficiently expressed at the cell surface independently of TAP and endogenous β2-microglobulin, and it was recognized directly and efficiently by specific T cells in vitro. A recombinant vaccinia virus encoding the Db NP SCT primed a CD8+ T cell response in C57BL/6 mice 4-fold greater than an equivalent virus expressing the NP epitope as a minigene, as shown by tetramer staining, whether or not the minigene was directed into the endoplasmic reticulum by a signal sequence. This response was functional as shown by in vivo lysis assays with peptide-pulsed target cells, and it was greatly expanded following secondary challenge in vivo with influenza virus. The SCT was also significantly more immunostimulatory for CD8+ cells than the NP minigene in adoptive transfer experiments using F5 TCR transgenic spleen cells, in which the magnitude of the T cell response was much greater. Our results extend previous DNA vaccination studies using SCTs, which demonstrated that such molecules are capable of generating functional CD8+ T cell responses. We have shown that class I SCTs are more immunogenic than even preprocessed Ag in the form of an epitope minigene, and they therefore should be considered for use when the generation of optimal CD8+ T cell responses is required.

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Section: T Here Is Continued Interest In Improving the Methods Availamentioning

confidence: 99%

“…Previous studies using MHC class I SCTs for priming in vivo have focused on using DNA vaccines (5)(6)(7)(8)(9). Although this approach has some advantages, the CD8 ϩ T cell responses generated are relatively weak, at least compared with the responses made against viruses.…”

Section: H-2d B Np Sct Primes a Vigorous Cd8 ϩ T Cell Response In Vivomentioning

confidence: 99%

A Single-Chain H-2Db Molecule Presenting an Influenza Virus Nucleoprotein Epitope Shows Enhanced Ability at Stimulating CD8+ T Cell Responses In Vivo

Palmowski

Parker

Choudhuri

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Anti-inflammatory agents have also been used for clinical treatment (Fujii et al, 2004;Groneberg et al, 2005;Lai, 2005;So et al, 2003;Tsang and Seto, 2004;Tsang and Zhong, 2003;van Vonderen et al, 2003). SARS vaccines are currently under development and evaluation (Bai et al, 2008;Cheung et al, 2007;Groneberg et al, 2005;Jiang et al, 2005b;Lin et al, 2007;Marshall and Enserink, 2004;Martin et al, 2008;Okada et al, 2007;Oxford et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies

Fang

Lin

Liao

et al. 2010

Molecular Immunology

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Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection causes lung failure characterized by atypical pneumonia. We previously showed that antibodies against SARS-CoV spike domain 2 (S2) in the patient sera can cross-react with human lung epithelial cells; however, the autoantigen is not yet identified. In this study, we performed proteomic studies and identified several candidate autoantigens recognized by SARS patient sera in human lung type II epithelial cell A549. Among the candidate proteins, annexin A2, which was identified by mass spectrometry analysis and had the highest score by Mascot data search, was further characterized and investigated for its role as an autoantigen. By confocal microscopic observation, SARS patient sera and anti-S2 antibodies were co-localized on A549 cells and both of them were co-localized with anti-annexin A2 antibodies. Anti-annexin A2 antibodies bound to purified S2 proteins, and anti-S2 bound to immunoprecipitated annexin A2 from A549 cell lysate in a dose-dependent manner. Furthermore, an increased surface expression and raft-structure distribution of annexin A2 was present in A549 cells after stimulation with SARS-induced cytokines interleukin-6 and interferon-gamma. Cytokine stimulation increased the binding capability of anti-S2 antibodies to human lung epithelial cells. Together, the upregulated expression of annexin A2 by SARS-associated cytokines and the cross-reactivity of anti-SARS-CoV S2 antibodies to annexin A2 may have implications in SARS disease pathogenesis.

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“…DNA vaccination of animal with SARS-CoV N gene led to the production of N-specific antibodies and specific cytotoxic T lymphocytes (CTLs) response, or even the replicon inhibition of vaccinia virus expressing N protein [21][22][23]. A DNA vaccine encoding N epitope stimulated cytotoxic T cells to kill N protein-expressing cell [24]. N protein expressed from recombinant measles and baculovirus resulted in strong humoral and cellular immune response in mice [25,26].…”

Section: Introductionmentioning

confidence: 99%

Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice

Zheng

Yang

et al. 2009

Vaccine

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Vaccines produced in plant systems are safe and economical; however, the extensive application of plant-based vaccines is mainly hindered by low expression levels of heterologous proteins in plant systems. Here, we demonstrated that the post-transcriptional gene silencing suppressor p19 protein from tomato bushy stunt virus substantially enhanced the transient expression of recombinant SARS-CoV nucleocapsid (rN) protein in Nicotiana benthamiana. The rN protein in the agrobacteria-infiltrated plant leaf accumulated up to a concentration of 79 microg per g fresh leaf weight at 3 days post infiltration. BALB/c mice were intraperitoneally vaccinated with pre-treated plant extract emulsified in Freund's adjuvant. The rN protein-specific IgG in the mouse sera attained a titer about 1:1,800 following three doses of immunization, which suggested effective B-cell maturation and differentiation in mice. Antibodies of the subclasses IgG1 and IgG2a were abundantly present in the mouse sera. During vaccination of rN protein, the expression of IFN-gamma and IL-10 was evidently up-regulated in splenocytes at different time points, while the expression of IL-2 and IL-4 was not. Up to now, this is the first study that plant-expressed recombinant SARS-CoV N protein can induce strong humoral and cellular responses in mice.

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Induction of T-cell response by a DNA vaccine encoding a novel HLA-A*0201 severe acute respiratory syndrome coronavirus epitope

Cited by 47 publications

References 36 publications

A Single-Chain H-2Db Molecule Presenting an Influenza Virus Nucleoprotein Epitope Shows Enhanced Ability at Stimulating CD8+ T Cell Responses In Vivo

A Single-Chain H-2Db Molecule Presenting an Influenza Virus Nucleoprotein Epitope Shows Enhanced Ability at Stimulating CD8+ T Cell Responses In Vivo

Annexin A2 on lung epithelial cell surface is recognized by severe acute respiratory syndrome-associated coronavirus spike domain 2 antibodies

Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice

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