Induction of systemic resistance in blackgram (Vigna mungo) against urdbean leaf crinkle virus by chemicals

Abstract

The effect of chemicals on the induction of systemic resistance in blackgram (Vigna mungo) against urdbean leaf crinkle virus (ULCV) was studied. Pre-inoculation spraying of blackgram plants with resistance-inducing chemicals, namely salicylic acid and benzothiadiazole at 100 ppm concentration was found to be effective in reducing ULCV infection and increasing the incubation period of the virus under controlled conditions. The treatment of blackgram plants with salicylic acid and benzothiadiazole significantly increased the phenylalanine ammonialyase (PAL), peroxidase (PO) and polyphenol oxidase (PPO) activities. New isoforms of peroxidase and polyphenol oxidase were also induced by these treatments. A two-fold increase in accumulation of total phenol was observed in plants treated with salicylic acid and benzothiadiazole.

Salicylic Acid, Phosphorous Acid and Fungicide Sumi 8 Effects on Polyphenol Oxidases Activities and Cassava Resistance to Anthracnose

“…This result could be explained by the fact that these plants had already set up their defense system after different treatments. Similar results were obtained by several authors [22] [23]. They advocated pretreatment method with elicitors in plants as a means to fight against plant diseases.…”

“…They advocated pretreatment method with elicitors in plants as a means to fight against plant diseases. In bean (Vigna mungo), pretreatment of plant with SA 24 hours before virus (urdbean leaf crinke virus) inoculation, has helped reduce disease symptoms while contaminated plants at same time to SA treatment developed significant symptoms [23]. Our results indicate that in cassava, a long period of pretreatment may be recommended to reduce anthracnose impact.…”

“…In elicited plants, PPO 10 was identified by the presence of AP (lines 6 and 7) and SA after 5 days of infection (line 4) (Figure 3). Examples of PPO isoenzymes induction were mentioned by Niranjan-Raj et al [24], Karthiekeyan et al [23] and, Wu and Duan [20] in bean (V. mungo) and millet (Glycine max). These authors noted the timeliness and amplification of PPO activities in resistant varieties compared to susceptible varieties.…”

“…Fifth PPO has been induced during Sclerospora graminicola inoculation. In V. mungo, new forms were induced after treatment plants with SA and benzothiadiazole [23].…”

Biochemical attributes of Indian mustard (Brassica juncea) and rapeseed (B. napus) as influenced by salicylic acid and benzothiadiazole

“…Salicylic acid (SA) is an important signaling molecule in plants and induces plant tolerance to various biotic and abiotic stresses (Horvath et al, 2007). Benzothiadiazole (BTH) [S-methylbenzo-1,2,3-thiadiazole-7 -carbothiate] is a chemical analogue of SA and has successfully been used to induce resistance to a wide range of diseases in field crops (Karthikeyan et al, 2009;Abdel-Monaim et al, 2011). Considering the importance of oilseeds, the objective of this study was to study the effect of foliar spray of salicylic acid and benzothiadiazole on seed oil, total soluble protein and glucosinolate content in B. juncea and B. napus cultivars.…”