Induction of synthesis of bacterial protein by excretory product of the alga Chlamydomonas reinhardtii y-1

Stegeman, William J.; Hoober, J. Kenneth

doi:10.1038/257244a0

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…As a result, the only significant radioactivity in protein on the gels was that incorporated into the induced polypeptide. However, a treatment with lysozyme permitted the solubilization of total cellular protein by SDS (28). The treatment with lysozyme resulted in markedly greater amounts of other polypeptides entering the gel during electrophoresis, but this treatment did not increase the recovery of the induced polypeptide from the cells.…”

Section: Resultsmentioning

confidence: 99%

“…Stegeman and Hoober (28) found that the incubation of a mixed culture of the alga Chlamydomonas reinhardtii y-1 with a bacterium, tentatively identified as a species ofArthrobacter, resulted in the induction of synthesis of a polypeptide in the bacterial cells. Stimulation of the synthesis of this polypeptide required light and was mediated by an excretory product of the algal cells.…”

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confidence: 99%

“…The cells were grown at 25°C in liquid culture and collected as etiolated cells after growth in the dark for 4 days (10). The bacterium, a coryneform type isolated as a contaminant of the algal culture (28), was grown at 25°C in algal medium supplemented with 0.1% yeast extract (Difco). The bacterium could not be maintained on the unsupplemented algal medium unless the algal cells were present.…”

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confidence: 99%

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Photodynamic induction of a bacterial cell surface polypeptide

Hoober¹

1977

J Bacteriol

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The photodynamic action of several dyes on cells of a bacterium, tentatively identified as a species of Arthrobacter, resulted in remarkable stimulation of synthesis of a polypeptide 21,000 daltons in mass. This polypeptide resides on the cell surface and can be solubilized by sodium dodecyl sulfate without lysis of the cells. Chlorophyllin and rose bengal are effective in inducing synthesis of the polypeptide in proportion to their ability to sensitize the photooxidation of histidine. Etiolated cells of the alga Chlamydomonas reinhardtii y-1 excrete a substance into the medium that also sensitized the photoinduction of the polypeptide.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Photodynamic induction of a bacterial cell surface polypeptide

Hoober¹

1977

J Bacteriol

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“…When the bacterial cells were exposed to light in the presence of a dye, synthesis of a polypeptide 21,000 daltons in mass (hereafter referred to as the 21K polypeptide) was markedly stimulated (14). As a result, this polypeptide became a major product of total cellular protein synthesis (35). When cells were treated with SDS (1 to 2%), a group of polypeptides, including the 21K polypeptide, was extracted (14).…”

Section: Resultsmentioning

confidence: 99%

Kinetics of accumulation of a photodynamically induced cell-surface polypeptide in a species of Arthrobacter

Hoober¹

1978

J Bacteriol

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Cells of a species of Arthrobacter were incubated in the light with methylene blue, a dye that sensitizes photooxidative reactions by the production of singlet oxygen. An early and major response by the cells to these conditions was stimulation of synthesis of a single cell-surface polypeptide, 21,000 daltons in mass. The rate of synthesis of this polypeptide reached a maximal level about 30 min after the start of illumination. As a consequence, the amount of this polypeptide increased at least 10-fold during a period of 5 h. The presence of histidine or methionine, scavengers of singlet oxygen, markedly diminished synthesis and accumulation of this polypeptide. Concomitant with the accumulation of this polypeptide on the cell surface was the appearance of an extensive array of pili.containing 1 mM Na2HPO4, 0.2 mM KH2PO4, 1 mM (NH4)2SO4, 1 mM MgCl2, and 0.03 mM CaCl2. Finally, the celLs were suspended in the deficient medium 359 on July 16, 2020 by guest

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“…2 A). The use of [aH]leucine to assay synthesis of the major polypeptides of the thylakoid membranes provided the opportunity to perform the analyses on total protein, thus making isolation of i The suggestion (45) that [aH]leucine was selectively incorporated into products of mitochondrial protein synthesis was in error, as described elsewhere (46). 2 If leucine entered the cells by simple passive diffusion, increasing the extraceUular concentration by the addition of unlabeled leucine should proportionally increase the total influx, without decreasing the amount of [aH]leucine entering the cells.…”

Section: Labeling Of Polypeptides During Greeningmentioning

confidence: 99%

Kinetics and regulation of synthesis of the major polypeptides of thylakoid membranes in Chlamydomonas reinhardtii y-1 at elevated temperatures

Hoober¹

1976

The Journal of Cell Biology

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Etiolated cells of Chlamydomonas reinhardtii y-1 exhibit rapid and linear initial kinetics of greening when exposed to light at 38~ The initial rate of chlorophyll accumulation under these conditions is greater than the maximal rate during greening at 25~ Synthesis of the major polypeptides of thylakoid membranes within intact cells was assayed during greening by the incorporation of [3H]leucine and the subsequent electrophoresis of total cellular protein on polyacrylamide gels in the presence of sodium dodecyl sulfate. At 38~ the major membrane polypeptides (about 28,000 and 24,000 daltons in mass) were synthesized at a linear rate after exposure of the cells to light, with no evidence of a lag period. A 1-2 h preincubation in the dark at the higher temperature was necessary to achieve linear initial kinetics. Actinomycin D inhibited synthesis of the membrane polypeptides if added at the beginning of a 2 h dark preincubation, but not when added near the end. These results suggested that transcription of the messenger RNA for the membrane polypeptides occurred during the dark period at 38~ But the major membrane polypeptides were not made by y-1 cells in the dark. The wavelengths of light most effective in eliciting production of the membrane polypeptides were the same as those allowing chlorophyll synthesis. In contrast, wild type cells, which are capable of chlorophyll synthesis in the dark, also make the membrane polypeptides in the dark. The data indicate that at elevated temperatures synthesis of the major thylakoid membrane polypeptides is controlled at a posttranscriptional step, and that this reaction normally proceeds only under conditions which permit reduction of protochlorophyllide.Greening of etiolated plant cells is characterized by the development of photosynthetic competency. The elemental process in the course of this development is the formation of thylakoid membranes within chloroplasts in response to light. Thylakoid membranes, which convert light energy into chemical energy, constitute the major system of membranes within light-grown plant cells. Although these membranes are located within the chloroplast, their formation requires the participation of synthetic systems in the cytoplasmic matrix as well as in the chloroplast. Chlorophyll and 326

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Induction of synthesis of bacterial protein by excretory product of the alga Chlamydomonas reinhardtii y-1

Cited by 8 publications

References 12 publications

Photodynamic induction of a bacterial cell surface polypeptide

Photodynamic induction of a bacterial cell surface polypeptide

Kinetics of accumulation of a photodynamically induced cell-surface polypeptide in a species of Arthrobacter

Kinetics and regulation of synthesis of the major polypeptides of thylakoid membranes in Chlamydomonas reinhardtii y-1 at elevated temperatures

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