Induction of synapse associated protein 102 expression in cyclosporin A‐stimulated hair growth

Abstract

Cyclosporin A (CsA) has been used as a potent immunosuppressive agent for inhibiting the graft rejection after organ transplantation. However, CsA provokes lots of side effects including hirsutism, the phenomenon of abnormal hair growth in the body. In the present study, we investigated the hair growth stimulating effect of CsA using in vivo and in vitro test models. When topically applied on the back skin of mice, CsA induced fast telogen to anagen transition. In contrast, CsA had no effect on the growth of human hair follicle tissues cultured in vitro, indicating that it might not have the mitogenic effect on hair follicles. To identify the genes related with CsA-induced hair growth, we performed differential display RT-PCR. Among the genes obtained, the expression of synapse associated protein 102 (SAP102) was verified using competitive RT-PCR. The result showed that the expression of SAP102 was significantly induced by CsA treatment in the back skin of C57BL/6 mice. However, the increase of SAP102 mRNA was also seen in spontaneous anagen mice, suggesting that induction of SAP102 is one event of the anagen hair growth response regardless of how the growth state was induced. SAP102 was not expressed in cultured human hair outer root sheath and dermal papilla cells. Immunohistochemistry analysis showed that CsA induced the expression of SAP102 in perifollicular region of mouse anagen hair. Together, these results suggest that SAP102 is one of hair-cycle-dependent genes, whose expression is related with the anagen progression.

Paradoxical Hypertrichosis After Laser Therapy: A Review

“…Hypertrichosis results when telogen or resting follicles are stimulated into anagen (active growth) or when nonpigmented vellus hair follicles are converted into longer, darker terminal hair follicles. Hypertrichosis can be congenital (hypertrichosis lanuginosa) or acquired (iatrogenic) from medications such as cyclosporine, steroids, penicillin, and streptomycin 3–5 . It can also occur as sequelae from underlying medical conditions, such as thyroid disease and porphyria 2 .…”

Reconstitution of the In Vitro Activity of the Cyclosporine-Specific P450 Hydroxylase from Sebekia benihana and Development of a Heterologous Whole-Cell Biotransformation System

“…Despite its immunosuppressant activity, CsA provokes several side effects, such as nephrotoxicity, hypertension, and hirsutism (4,5). Among these side effects, the strong hairgrowth-stimulating activity of CsA has attracted significant attention from both the academic community and the cosmetics industry due to its great potential to treat alopecia (6,7). Mechanistically, it was revealed that CsA stimulates the growth of both murine hair epithelial cells and epidermal keratinocytes at an optimum concentration through inhibiting the expression and translocation of some protein kinase C isozymes that act as negative hair-growing factors (8).…”

Hair follicular cell/organ culture in tissue engineering and regenerative medicine

“…However, CsA provokes many side effects, including hirsutism, which is the phenomenon of abnormal hair growth on the body. In the present study, we investigated the stimulation of hair growth by CsA using hair epithelial cells [54]. Finally Shin et al reconstructed DP like tissues of DPCs and DSCs using a special medium and growth factors in vitro.…”