Induction of stress fibres and intercellular gaps in human vascular endothelium by shock-waves

Seidl, Markus; Steinbach, Pia; Wörle, Klaus; Hofstädter, Ferdinand

doi:10.1016/0041-624x(94)90111-2

Cited by 78 publications

(49 citation statements)

References 9 publications

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“…We focused on the effects of ESWT on the adhesion and migration of osteoblasts while aiming to reveal the underlying specific biochemical signals triggered by ESW. Different shock waves could induce different outcomes (15,32). In our experiments, dose-dependent complications were observed, and it was determined that optimal intensity of energy was 500 impulses at 10 kV.…”

Section: Discussionmentioning

confidence: 96%

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Chen

et al. 2012

Journal of Biological Chemistry

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Background:The effects of extracorporeal shock wave (ESW) on adhesion and migration of osteoblasts have not been reported until now. Results: Optimal intensity shock wave promotes osteoblast adhesion and migration. Conclusion: ESW promotes the adhesion and migration of osteoblasts via integrin ␤1-mediated expression of phosphorylated FAK.Significance: This provides a mechanistic basis for improving the effectiveness of ESW treatment in fracture healing and tissue engineering.

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Section: Discussionmentioning

confidence: 96%

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Chen

et al. 2012

Journal of Biological Chemistry

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“…Because the physical forces generated by cavitation are highly localized, SW could induce localized stress and subsequent shear stress on cell surface membranes (Fisher et al 2001). Several biochemical effects of SW were reported including hyperpolarization, Ras activation, non-enzymatic NO synthesis, and induction of stress fibers and intercellular gaps (Seidl et al 1994;Wang et al 2001;Gotte et al 2002). Also, SW treatment is reported to affect the expression of several chemokines and matrix metalloproteinases and therefore bring anti-inflamatory effects Mariotto et al 2005;Stojadinovic et al 2008;Mariotto et al 2009) in addition to up-regulation of VEGF and its receptor Flt-1 (Nishida et al 2004), implying that the multiple angiogenic pathways are involved in the beneficial effects of the SW therapy.…”

Section: Mechanisms For Sw-induced Neovascularizationmentioning

confidence: 99%

Extracorporeal Shock Wave Therapy as a New and Non-invasive Angiogenic Strategy

Ito

Shimokawa

2009

Tohoku J. Exp. Med.

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“…Enhanced cellular proliferation and regeneration by proliferating cell nuclear antibody (PCNA) and insulin-like growth factor (IGF-I) were demonstrated after ESWA of rat Achilles tendons [8]. Furthermore ESWA has been shown to exert a significant synergistic growth inhibitory effect on tumour cells when applied in combination with chemotherapeutic agents [9,10], which was explained by CP-induced permeabilization of cellular membranes and intercellular connections [11,12]. Recent investigations revealed decreased leukocytic infiltration and suppression of tumour necrosis factor a (TNF-a) in critically perfused random skin flaps after ESWA [13].…”

Section: U N C O R R E C T E D P R O O Fmentioning

confidence: 96%

Microvascular Response to Shock Wave Application in Striated Skin Muscle

Calcagni

Chen

Högger

et al. 2011

Journal of Surgical Research

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Background: This study aims to quantify by intravital microscopy the microhemodynamic response after extracorporeal shock wave application (ESWA) to the physiologic microcirculation of the mouse dorsal skinfold chamber.Materials and Methods: ESWA was carried out using an electrohydraulic shock wave source. Two different shock wave doses of 500 and 1000 pulses at an energy flux rate of 0.08 mJ/mm2 and a frequency of 4 Hz were compared with sham-operated animals. Microcirculatory analyses were performed at baseline (BL) and during a 3 d observation period after ESWA. The expression of caspase-3 (casp-3), proliferating cell nuclear antibody (PCNA), von Willebrand factor (vWF), and endothelial nitric oxide synthase (eNOS) were analyzed semiquantitatively by immunohistochemistry.Results: ESWA provoked a significant and persistent increase of functional capillary density (FCD) throughout the observation period, reaching a maximum (140% ± 5% of BL, P < 0.05 versus sham) after 1 d when animals were treated with 1000 pulses. ESWA induced a slight increase of leukocyte rolling (not, vert, similar2-to not, vert, similar3.5-fold, P < 0.05) and leukocyte adherence (not, vert, similar1.5-to not, vert, similar2-fold, P < 0.05) to the endothelial lining of postcapillary venules. One day following ESWA, we observed enhanced expression of casp-3 (not, vert, similar3-to not, vert, similar4-fold), PCNA (not, vert, similar9-to not, vert, similar14-fold), vWF (not, vert, similar11-to not, vert, similar14-fold), and eNOS (not, vert, similar3-fold), all P < 0.05.Conclusion: This study shows that ESWA provokes a favorable persistent increase of patent capillaries, however accompanied by a transient and slight inflammatory response but also by dose-dependant apoptotic cell death. Our data suggest that ESWA might represent a noninvasive biomechanical tool to treat critically perfused and endangered tissues, but certainly warrants further investigation. Background. This study aims to quantify by intravital microscopy the microhemodynamic response after extracorporeal shock wave application (ESWA) to the physiologic microcirculation of the mouse dorsal skinfold chamber. U N C O R R E C T E D P R O O FMaterials and Methods. ESWA was carried out using an electrohydraulic shock wave source. Two different shock wave doses of 500 and 1000 pulses at an energy flux rate of 0.08 mJ/mm 2 and a frequency of 4 Hz were compared with sham-operated animals. Microcirculatory analyses were performed at baseline (BL) and during a 3 d observation period after ESWA. The expression of caspase-3 (casp-3), proliferating cell nuclear antibody (PCNA), von Willebrand factor (vWF), and endothelial nitric oxide synthase (eNOS) were analyzed semiquantitatively by immunohistochemistry.Results. ESWA provoked a significant and persistent increase of functional capillary density (FCD) throughout the observation period, reaching a maximum (140% ± 5% of BL, P < 0.05 versus sham) after 1 d when animals were treated with 1000 pulses. ESWA induced a slight increase of ...

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Induction of stress fibres and intercellular gaps in human vascular endothelium by shock-waves

Cited by 78 publications

References 9 publications

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Optimal Intensity Shock Wave Promotes the Adhesion and Migration of Rat Osteoblasts via Integrin β1-mediated Expression of Phosphorylated Focal Adhesion Kinase

Extracorporeal Shock Wave Therapy as a New and Non-invasive Angiogenic Strategy

Microvascular Response to Shock Wave Application in Striated Skin Muscle

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