Induction of Staphylococcus aureus-specific IgA and agglutination potency in milk of cows by mucosal immunization

Plat-Sinnige, Marjan J. Tempelmans; Verkaik, Nelianne J.; Wamel, Willem J. B. van; Groot, Nanda de; Acton, Dennis S.; Belkum, Alex van

doi:10.1016/j.vaccine.2009.04.034

Cited by 9 publications

(1 citation statement)

References 47 publications

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“…In addition, the nasal cavity is one of the most promising administration sites because the oral route has high digestive enzymatic activity and may dissolve vaccine antigens. Interestingly, a previous study reported that cows, which were immunized via the mucosa (including intranasally) with inactivated S. aureus strains, presented S. aureus -specific IgA antibodies in the milk [33], confirming that nasal immunization with inactivated S. aureus could actually induce S. aureus -specific IgA antibodies in the mammary gland. In contrast, mucosal immunization generally induces mucosal-specific IgA antibodies and serum-specific IgG antibodies; however, the OD value of anti- S. aureus -specific IgG antibodies in composite milk did not differ significantly in this study (Fig.…”

Section: Discussionmentioning

confidence: 79%

Staphylococcus aureus-specific IgA antibody in milk suppresses the multiplication of S. aureus in infected bovine udder

et al. 2019

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Background Bovine mastitis caused by Staphylococcus aureus ( S. aureus ) is extremely difficult to control and new methods for its prevention and management are required. Nasal vaccines may prevent initial bovine mastitis infection caused by S. aureus . However, limited information is available regarding induction of mucosal immune response through nasal immunization with antigen and its suppression of S. aureus multiplication during bovine mastitis. This study sought to investigate whether induction of immunoglobulin A (IgA) in milk by nasal immunization could suppress multiplication of S. aureus in the bovine udder. Results Nasal immunization with formalin-killed S. aureus conjugated with a cationic cholesteryl-group-bearing pullulan-nanogel was performed. Anti- S. aureus -specific IgA antibodies were significantly more abundant in the milk of immunized cows than in non-immunized animals ( P < 0.05). S. aureus counts in the quarter were negative in both non-immunized and nasal-immunized cows 1 week after mock infusion. In S. aureus -infused quarters, S. aureus multiplication was significantly suppressed in immunized compared with non-immunized cows ( P < 0.05). Furthermore, a significant negative correlation was found between S. aureus -specific IgA antibodies and S. aureus counts in infused quarters of both non-immunized and nasal-immunized cows (r = − 0.811, P < 0.01). Conclusion In conclusion, the present study demonstrates that S. aureus -specific IgA antibodies in milk successfully suppressed the multiplication of S. aureus in infected bovine udders. Although the exact mechanism explaining such suppressive effect remains to be elucidated, nasal vaccines that can induce humoral immunity may help prevent initial infection with S. aureus and the onset of bovine mastitis. Electronic supplementary material The online version of this article (10.1186/s12917-019-2025-3) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 79%

Staphylococcus aureus-specific IgA antibody in milk suppresses the multiplication of S. aureus in infected bovine udder

et al. 2019

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Novel Technology to Study Co-Evolution of Humans and Staphylococcus aureus: Consequences for Interpreting the Biology of Colonisation and Infection

Belkum

2010

Advances in Experimental Medicine and Biology

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Human nasal carriage of Staphylococcus aureus is a textbook example of an apparently neutral interaction between humans and a bacterial species that can still lead to (severe) opportunistic infections. The co-evolutionary aspects of this interaction are slowly surfacing, facilitated by the emergence of a diversity of diagnostic, epidemiological and molecular research tools. Basic microbiology has helped define persistent vs. non-carriage and the genotype of both host and guest has been explored in search of genetic markers for bacterial persistence. This chapter summarises the current state of affairs relating to artificial human colonisation studies with S. aureus, large-scale human cohort studies and innovative assessment of the humoral immune status of (non-)nasal carriers. These experimental approaches have recently assisted in identifying bacterial and human determinants and risk factors for staphylococcal carriage. Further refinement of the model by describing the interactions between S. aureus and its human host in molecular detail is important since it may pinpoint novel anti-infectious strategies.

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A reply to the comments on “Control of bovine mastitis in the 21st century: Immunize of tolerize?”

Rainard

2019

Research in Veterinary Science

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Induction of Staphylococcus aureus-specific IgA and agglutination potency in milk of cows by mucosal immunization

Cited by 9 publications

References 47 publications

Staphylococcus aureus-specific IgA antibody in milk suppresses the multiplication of S. aureus in infected bovine udder

Staphylococcus aureus-specific IgA antibody in milk suppresses the multiplication of S. aureus in infected bovine udder

Novel Technology to Study Co-Evolution of Humans and Staphylococcus aureus: Consequences for Interpreting the Biology of Colonisation and Infection

A reply to the comments on “Control of bovine mastitis in the 21st century: Immunize of tolerize?”

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