Induction of Somatic Embryogenesis in Saffron Using Thidiazuron (Tdz)

Sheibani, M.; Nemati, Seyyed Hossein; Davarinejad, G.H.; Azghandi, Ali Vatanpour; Habashi, Ali Akbar

doi:10.17660/actahortic.2007.739.32

Cited by 28 publications

(17 citation statements)

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“…Complete plantlets with adventitious roots and corm formation were reported at low levels in the literature [11][12][13]. Regeneration via somatic embryogenesis has also been described from corm-derived callus cultures [14][15][16], from leaf explants [9], and from protoplast 6-benzylaminopurine (BAP) were best for callus initiation and growth while 1.5 mg L -1 BAP was excellent for high rate of adventitious shoot formation. 1 mg L -1 indole-3-butyric acid (IBA) was more preferable for adventitious corm and root initiation as well as growth.…”

Section: Introductionmentioning

confidence: 99%

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

2011

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The objective of this study was to investigate development of an efficient in vitro tissue culture system for saffron (Crocus sativus L.) complete with roots and corms. In indirect organogenesis, Murashige and Skoog (MS) media with 3% (w/v) sucrose, 100 mg L−1 ascorbic acid, and the combination of 0.25 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg L−1 6-benzylaminopurine (BAP) were best for callus initiation and growth while 1.5 mg L−1 BAP was excellent for high rate of adventitious shoot formation. 1 mg L−1 indole-3-butyric acid (IBA) was more preferable for adventitious corm and root initiation as well as growth. Overall, 64% rooting and 33% corm production rates were achieved in indirect organogenesis. In direct organogenesis, MS medium supplemented with 3 % sucrose, 100 mg L−1 ascorbic acid and 1 mg L−1 BAP was optimum for shoot growth. While 1 mg L−1 IBA was best for adventitious corm formation, 2 mg L−1 IBA promoted adventitious root initiation and growth. Overall, 36% and 57% of explants had corm and contractile root, respectively. The high rates suggest that efficient tissue culture system could be achieved for mass propagation and ex situ conservation of threatened saffron genetic resources.

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Section: Introductionmentioning

confidence: 99%

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

2011

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“…The high efficacy of TDZ for inducing somatic embryogenesis has already been demonstrated in several monocot and dicot plants [6,7,9,13,21,22]. In many cases, TDZ induced embryogenesis was reported to be of higher efficiency and frequency as compared to other cytokinins or the combination treatments of auxins and cytokinins [3,21,26,30].…”

Section: Discussionmentioning

confidence: 99%

Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (<i>Colocasia esculenta</i> var. <i>esculenta</i>)

Verma¹

2017

AJBIO

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This is a first report on direct somatic embryogenesis and organogenesis of taro (Colocasia esculenta var. esculenta) using axillary meristem explants. Best somatic embryogenesis was observed in cultures that were established on Murashige and Skoog (1962) (MS medium) containing 10 µM 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 2 µM thidiazuron (TDZ) and then transferred on medium with 5 µM TDZ. MS medium containing 3 µM gibberellic acid (GA) and 4.5% sucrose proved best for inducing germination in somatic embryos which converted into 20.0 ± 3.46 complete plantlets per embryo cluster per explant on ½ strength MS basal salts with 1% sucrose. Best organogenesis was observed in cultures that were established on MS medium containing 2 µM TDZ and then transferred on medium with 5 µM TDZ. Subsequent transfer of these cultures on MS medium with 5 µM indole-3-acetic acid (IAA) and 7.5 µM 6-benzylaminopurine (BAP) resulted in maximum shoot multiplication. MS medium containing 2 µM IAA proved best for inducing rooting in multiplied shoots. Both direct somatic embryogenesis and organogenesis resulted in mass and rapid production of taro plantlets which were acclimatized and field transferred. Vigorous plant growth and healthy corm production was observed in the field. This in vitro propagation method of taro through direct somatic embryogenesis and organogenesis is significantly reliable over prevailing methods available for other cultivars and provides sustainable means of quality taro production in the Pacific and the Caribbean region where non-availability of elite seedlings is a limiting issue.

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“…The medium was solidified with 0.7% agar and cultures were incubated in a growth chamber under dark conditions at 25ºC for callus induction. Different growth regulator combinations were tested for callus induction (Table 2) as suggested by Sheibani et al (2007), Darvishi et al (2007), Majourhay et al (2007), Sharifi and Ebrahimzade (2010), Blazquez et al (2009).…”

Section: Methodsmentioning

confidence: 99%

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

Vahedi

Kalantari

Salami

2014

Plant Tissue Cult. & Biotech.

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Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA. In some treatments, calli were transferred to organogenesis stage after two subcultures. For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ proved to be the best growth regulator treatment for shoot regeneration from the saffron calli.

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Induction of Somatic Embryogenesis in Saffron Using Thidiazuron (Tdz)

Cited by 28 publications

References 0 publications

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (<i>Colocasia esculenta</i> var. <i>esculenta</i>)

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

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Induction of Somatic Embryogenesis in Saffron Using Thidiazuron (Tdz)

Cited by 28 publications

References 0 publications

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Improved in vitro micropropagation method with adventitious corms and roots for endangered saffron

Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (&lt;i&gt;Colocasia esculenta&lt;/i&gt; var. &lt;i&gt;esculenta&lt;/i&gt;)

Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

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Direct Somatic Embryogenesis and Organogenesis from Axillary Meristem in Taro (<i>Colocasia esculenta</i> var. <i>esculenta</i>)