Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

Giri, Dinesh; Tamta, Sushma

doi:10.3329/ptcb.v23i2.17506

Cited by 10 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Somatic embryogenesis has already been reported in many endangered plants for their in vitro conservation (Lee et al, 2011;Kim et al, 2012;Giri et al, 2013;Barberini et al, 2016). In the present study, cytokinins were used as promoters for the induction of somatic embryos.…”

Section: Somatic Embryogenesis and Shoot Regenerationmentioning

confidence: 99%

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Mahajan¹,

Kapoor²

2016

JEBAS

View full text Add to dashboard Cite

Picrorhiza kurroa Royle ex. Benth is an endangered and important medicinal plant of alpine Himalayas. Developing an efficient protocol for its mass multiplication is essential to meet the requirements of pharmaceutical industries and also in conservation of this plant under its natural habitat. Present study was undertaken to develop a protocol for in vitro mass multiplication of P. kurroa. Result of study revealed that highest frequency of shoot regeneration was achieved on Murashige and Skoog's basal medium supplemented with 1.0mg/l BAP, 0.5 mg/l Kn and 1.0mg/l GA3, while the best rooting was observed in MS medium supplemented with 2.5 mg/l IBA. MS medium supplemented with 3.0 mg/l 2,4-D resulted in highest frequency of embryogenic callus. Callus inoculated on MS media supplemented with BAP and IAA resulted in both shoot and root formation while the callus on MS media supplemented with NAA and IBA resulted only root formation. The somatic embryos were established from callus on MS medium supplemented 2.5 mg/l 2,4-D after four weeks. MS medium containing 1.0 mg/l BAP and 1.0 mg/l GA3 resulted into shoots from well developed somatic embryos. This protocol will provide a system for the germplasm conservation in P. kurroa by multiplication and regeneration of true to type plants.

show abstract

Section: Somatic Embryogenesis and Shoot Regenerationmentioning

confidence: 99%

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Mahajan¹,

Kapoor²

2016

JEBAS

View full text Add to dashboard Cite

show abstract

“…TDZ induces the expression of speci c genes and transcription regulatory sequences that are either directly responsible for callus formation or induce other auxins or cytokinins for callus induction (Galán-Ávila et al 2020;Ali et al 2022). The higher concentration of NAA (20 µM) showed a better response to induce direct embryogenesis from the seed radicle of the zygotic embryo (Giri and Tamta 2013).However, a commercial-scale propagation protocol has not been developed so far. Previous studies attempted to optimize PGRs concentration for micropropagation of H.spicatum through shoot tip and rhizome bud explants (Koul et al 2005;Badoni et al 2010).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the method can be used for continuous, sustainable, economical and viable production of natural compounds, regardless of environmental conditions (Karuppusamy 2009). The in vitro propagation methods are available for the species (Badoni et al 2010;Giri and Tamta, 2011;2013); however, the propagation protocol of the species at the commercial level has yet to be developed.…”

Section: Introductionmentioning

confidence: 99%

Efficient plant regeneration through callus culture in Hedychium spicatum Buch.Ham. ex. D. Don using response surface methodology

Dhyani,

Bhatt,

Pandey

2024

Preprint

View full text Add to dashboard Cite

Hedychium spicatum(Family-Zingiberaceae), commonly known as Kapoor Kachri is widely known for its medicinal properties and high market demand. The species is harvested mainly from the wild to meet the raw material requirements for the pharmaceutical and cosmaceutical industries; therefore, it needs urgent attention for its conservation and mass production. The present study developed an efficient in vitro propagation protocol for large-scale species production. The central composite design- response surface methodology (CCD-RSM) experiment was designed to optimize the plant growth regulators (PGRs) concentration for maximum callus production, shoot regeneration and rooting. Seed radicle was used as explants in Murashige and Skoog (MS) medium supplemented with different concentrations of naphthalene acetic acid (NAA; 2.5-5.0 µM) in combination with thidiazuron (TDZ; 2.5-5.0 µM) for callus induction. TDZ (5µM) with NAA (2.5µM) showed maximum callus induction (98%) after 6 weeks of incubation. Callus pieces were transferred to MS medium supplemented with different concentrations of TDZ, NAA and Indole-3-butyric acid (IBA) for shoot regeneration. The highest regeneration frequency (100 %) was observed on MS medium enriched with 2.5 µM TDZ and 3.5 µM NAA that showed a maximum number of shoots/explants (16.19 no.). Regenerated shoots were rooted better (average number of roots/shoot - 11.71) on MS medium supplemented with 2 µM NAA and 1.5 µM IBA in combination. After subsequent acclimatization and hardening process in the greenhouse, the plantlets were planted in the experimental field with a survival rate of 83% after 4 months. The protocol established in the present study has prospects to meet the challenges of quality planting material for large-scale cultivation and raw material sources for industrial utilization.

show abstract

“…Shoot elongation and higher shooting percentage were also reported in MS medium supplemented with 5.0 m m kinetin and 1.0 m m IAA . Direct somatic embryos were developed by Giri and Tanta (2013) on cotyledon explants of zygotic embryos on MS supplemented with a high concentration of NAA (20.0 μ m ) . Induction of high frequency shoot multiplication (83.33%), number of shoots per explant (3.86 shoots) and average number of shoots per flask (19.33 shoots) were reported in selected cytokines combinations when seed raised shoot tips of the species were taken as explants .…”

Section: Propagation Variety Improvement and Market Demandmentioning

confidence: 99%

Hedychium spicatum: a systematic review on traditional uses, phytochemistry, pharmacology and future prospectus

Rawat

Jugran

Bhatt

et al. 2018

Journal of Pharmacy and Pharmacology

View full text Add to dashboard Cite

Literature reveals that the species is lacking in exact scientific basis of the beneficial properties. Although, some other distinct biological properties identified in this species also opened new door way for its new applications. Therefore, the mentioned phytochemical constituents such as phenolic and flavonoids compounds; and related pharmacological activities such as antimicrobial, anti-inflammatory and antioxidant activity of the species have provided some suggestive scientific evidences for its potential in pharmaceutical, food and aromatic industries.

show abstract

Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

Cited by 10 publications

References 11 publications

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Somatic embryogenesis and callus proliferation in Picrorhiza kurroa Royle ex. Benth

Efficient plant regeneration through callus culture in Hedychium spicatum Buch.Ham. ex. D. Don using response surface methodology

Hedychium spicatum: a systematic review on traditional uses, phytochemistry, pharmacology and future prospectus

Contact Info

Product

Resources

About