Induction of shoot buds, multiplication and plantlet formation in seedling explants of bell pepper (Capsicum annuum L.) cv. Bryza in vitro

Abstract

In vitro shoot bud induction and multiplication as well as plantlets formation from different parts of 21-d old seedlings (shoot tip, cotyledonary node, distal part of cotyledon, acropetal section of hypocotyl) of <em>Capsicum annuum</em> L., cv. Bryza were compared. During 4 weeks of primary explant culture on initiation media, first shoot bud primordia appeared; they reminded leaf primordia and subsequently some of them underwent enlargement, some developed into leaves and leaf-like structures (mainly on cotyledon explants). The highest number of shoot bud primordia was noted on cotyledonary node explants, but they were smaller than those on the remaining types of the explants. The best response of shoot regeneration showed cotyledon explants on which most of shoot buds were formed in each from four treated passages. From shoot buds on elongation media after 4 weeks of culture rooted rosettes of leaves were achieved, and the extension of the culture time to eight weeks without subculture caused that the rosettes developed into plantlets. Throughout four successive passages plantlets were obtained from cotyledon and shoot tip explants

In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

“…The pattern of pepper regeneration stages observed in our study was typical for the species, similar to observations made previously by Gatz (2002aGatz ( , 2002b, Qin et al (2005), and Joshi and Kothari (2007). The clusters of sessile leaves were formed during the induction of organogenesis, while the formation of rooted, properly foliaged shoots with the apex was induced only after transferring the explants onto the successive medium.…”

“…In consequence, effective methods have been developed for specific cultivars that recommend necessary modifications for particular genotypes (Dabauza and Peña, 2001). In general, it was found that both 6-benzylaminopurine (BA) and indole acetic acid (IAA) are effective growth regulators in the first stage of organogenesis induction (Phillips and Hubstenberger, 1985;Rajam, 1994, 1996;Gatz, 2002b;Paz and Castañeda, 2004;Qin et al, 2005;Sanatombi and Sharma, 2007). The second stage, elongation, during which leafy shoots are formed, is the critical phase in pepper in vitro cultures, causing unsatisfactory propagation coefficients and precluding the opportunity of application of this technology on a production scale (Ochoa-Alejo and Ireta-Moreno, 1990;Valera-Montero and Ochoa-Alejo, 1992;Frank-Duchenne et al, 1998;Husain et al, 1999;Gatz, 2002b;Joshi and Kothari, 2007).…”

“…In consequence, effective methods have been developed for specific cultivars that recommend necessary modifications for particular genotypes (Dabauza and Peña, 2001). In general, it was found that both 6-benzylaminopurine (BA) and indole acetic acid (IAA) are effective growth regulators in the first stage of organogenesis induction (Phillips and Hubstenberger, 1985;Rajam, 1994, 1996;Gatz, 2002b;Paz and Castañeda, 2004; Abstract: This study evaluates the effectiveness of in vitro regeneration of 4 genotypes of pepper (Capsicum spp.) that differ in origin and functional properties: California Wonder, (ATZ × Sono)F 1 , Jalapeno, and SF-9.…”

“…Qin et al, 2005;Sanatombi and Sharma, 2007). The second stage, elongation, during which leafy shoots are formed, is the critical phase in pepper in vitro cultures, causing unsatisfactory propagation coefficients and precluding the opportunity of application of this technology on a production scale (Ochoa-Alejo and Ireta-Moreno, 1990;Valera-Montero and Ochoa-Alejo, 1992;Frank-Duchenne et al, 1998;Husain et al, 1999;Gatz, 2002b;Joshi and Kothari, 2007). The shoot differentiation generally happens in rooting cultures or additional cultures stimulating elongation, and sometimes simply during acclimatization (Phillips and Hubstenberger, 1985;Arrollo and Revilla, 1991;Ebida and Hu, 1993;Franck-Duchenne et al, 1998;Dabauza and Peña, 2001;Gatz, 2002b;Song et al, 2010).…”