Induction of secretory leukocyte protease inhibitor (SLPI) in estradiol valerate (EV) induced polycystic ovary

Park, Jin Ju; Bae, Chun Sik; Choi, Byeong-Dae; Jeong, Soon-Jeong; Wang, Guanlin; Lim, Do-Seon; Kim, Byung-Ock; Cho, Young-Sik; Kim, Sun-Ju; Jeong, Moon‐Jin

doi:10.1007/s12272-011-0820-x

Cited by 5 publications

(6 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, delayed healing in PCOS rat skin wound is consistent with the results showing a large wound area, slow re-epithelization, increased inflammation and matrix degradation. Interestingly, SLPI mRNA and protein expression was higher in the PCOS rat skin than the normal rat skin, and also increased in the PCOS group after wounding, which is consistent with our previous results of SLPI induction in a polycystic ovary (4). Recently, microarray and qPCR data for estrogen-regulated gene expression in old and young human subjects demonstrated that SLPI expression is higher in old human skin than in young human normal skin, which is consistent with the present result (27) and the unpublished results for SLPI expression in OVX mouse normal skin (data not shown).…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

Delayed healing and induction of secretory leukocyte protease inhibitor in polycystic ovary syndrome rat skin wounds

Jeong

Kim²,

Bae³

et al. 2011

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

Abstract. Secretory leukocyte protease inhibitor (SLPI) and estrogen promote wound healing through a decrease in the excessive inflammatory response, accelerating re-epithelialization and increasing the amount of collagen deposition. The excessive administration of estradiol valerate (EV) using hormonal therapy decreases the concentration of estrogen abruptly and induces the polycystic ovary syndrome (PCOS). In this study, the PCOS rat skin wound area was wider than that of the normal groups and the rate of keratinocyte migration in PCOS was lower than the normal group. The numbers of inflammatory cells and macrophages recruited in the PCOS group were larger than that of the normal group. More collagen was deposited in the healing area of the normal group than in the PCOS group. The level of SLPI expression was higher in the PCOS group than the normal group after wounding, with the exception of the epithelium. On the other hand, mRNA and protein expression levels of transforming growth factor-β1 (TGF-β1) were lower in the PCOS group than in the normal group. Matrix metalloproteinase-2 (MMP-2) and MMP-9 levels in the PCOS group were significantly lower than that of the normal group. Therefore, increased SLPI in PCOS skin wounds may help prevent an excessive inflammatory response and aberrant collagen deposition but not are sufficient to accelerate PCOS skin wound healing, suggesting that SLPI may act as a local rather than a systemic modulating molecule in PCOS rat skin wounds.

show abstract

Section: Discussionsupporting

confidence: 92%

“…The antral-follicle, which produces androgen, is frequently observed in the PCOS ovary instead of the dominant preovulatory follicles (2)(3)(4). PCOS is a hormonal imbalance condition that affects the endocrine system and gives rise to complications, such as obesity and diabetes.…”

Section: Introductionmentioning

confidence: 99%

Delayed healing and induction of secretory leukocyte protease inhibitor in polycystic ovary syndrome rat skin wounds

Jeong

Kim²,

Bae³

et al. 2011

Int J Mol Med

Self Cite

View full text Add to dashboard Cite

show abstract

“…Additionally, SLPI is an anti-inflammatory mediator ( 41 ). SLPI can contribute to wound healing by decreasing the excessive inflammatory response, elevating keratinocyte proliferation and increasing collagen deposition by suppressing the activity of protease ( 42 ). To the best of our knowledge, the association between SLPI and PMOP has not been reported previously.…”

Section: Discussionmentioning

confidence: 99%

Identification of aberrantly expressed long non-coding RNAs in postmenopausal osteoporosis

Bai

Lin

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

Postmenopausal osteoporosis (PMOP) is a common skeletal disorder in postmenopausal women. The present study aimed to identify the key long non-coding RNAs (lncRNAs) in PMOP through RNA sequencing. RNA sequencing was performed to obtain the expression profile of lncRNAs and mRNAs in blood samples of patients with PMOP and normal controls (NCs). Following the identification of differentially expressed mRNAs (DEmRNAs) and differentially expressed lncRNAs (DElncRNAs), the DElncRNA-DEmRNA co-expression network was constructed. A search was performed for the DEGs transcribed within a 100-kb window upstream or downstream of DElncRNAs, which served as nearby DEmRNAs of DElncRNAs. Functional annotation of the DEmRNAs co-expressed with DElncRNAs was performed. The GSE56815 dataset was used to verify the expression of selected DEmRNAs and DElncRNAs. Three blood samples from patients with PMOP and two blood samples from NCs were used for RNA sequencing. Compared with the NC group, a total of 185 DEmRNAs and 51 DElncRNAs were obtained in PMOP. A total of 3,057 co-expression DElncRNA-DEmRNA pairs and 97 DElncRNA-nearby DEmRNA pairs were obtained. Six DEmRNAs [diacylglycerol O-acyltransferase 2, potassium voltage-gated channel subfamily S member 1, peptidase inhibitor 3, secretory leukocyte peptidase inhibitor, galectin-related protein and alkaline phosphatase, liver/bone/kidney (ALPL)] were nearby co-expressed genes of four DElncRNAs, including LOC105376834, LOC101929866, LOC105374771 and LOC100506113. Three PMOP-associated DEmRNAs, including ALPL, suppressor of cytokine signaling 3 and adrenomedullin, were co-expressed with the hub DElncRNAs (LINC00963, LOC105378415, LOC105377067, HCG27, LOC101928143 and LINC01094) of the positively and negatively co-expressed DElncRNA-DEmRNA interaction network. The expression of selected DEmRNAs and DElncRNAs was consistent with the RNA-sequencing results. In conclusion, the present study identified the key DEmRNAs and DElncRNAs in PMOP, which may provide clues for understanding the mechanism and developing novel biomarkers for PMOP.

show abstract

“…It is also likely that poly (I:C) increases the secretion of a wide range of antimicrobials and cytokines beyond those measured by us. Other known anti-HIV proteins such as SLPI and cathelicidin are expressed in the ovary [46, 47] and restrict HIV infection at other sites [48–50]. Therefore, the increased anti-HIV response could also be due to additional secreted antimicrobials and cytokines.…”

Section: Discussionmentioning

confidence: 99%

Poly (I:C) and LPS induce distinct immune responses by ovarian stromal fibroblasts

Patel

Shen

Wira

2018

Journal of Reproductive Immunology

View full text Add to dashboard Cite

Despite its anatomical location, the ovary is a site of pathogen exposure in the human female reproductive tract (FRT). However, the role of ovarian stromal fibroblasts in immune protection is unclear. We generated a population of ovarian stromal fibroblasts derived from normal human ovaries that expressed the pattern recognition receptors TLR3, TLR4, RIG-I, & MDA5. Poly (I:C) and LPS, respective mimics of viral and bacterial infections, selectively upregulated antiviral gene expression and secretion of chemokines and antimicrobials. Poly (I:C) exclusively stimulated the expression of interferon (IFN) β, IFNλ1, and the IFN-stimulated gene OAS2. Poly (I:C) also significantly increased secretion of elafin, CCL20, and RANTES, but had no effect on SDF-1α. In contrast, LPS had no effect on IFN or ISG expression but significantly increased secretion of RANTES and SDF-1α. Secretions from poly (I:C)-treated fibroblasts had both greater anti-HIV activity and induced higher levels of CD4 + T cell chemotaxis than those from LPS-treated cells. Our studies demonstrate a potential key role for ovarian fibroblasts in innate immune protection against incoming pathogens in the normal ovary.

show abstract

Induction of secretory leukocyte protease inhibitor (SLPI) in estradiol valerate (EV) induced polycystic ovary

Cited by 5 publications

References 36 publications

Delayed healing and induction of secretory leukocyte protease inhibitor in polycystic ovary syndrome rat skin wounds

Delayed healing and induction of secretory leukocyte protease inhibitor in polycystic ovary syndrome rat skin wounds

Identification of aberrantly expressed long non-coding RNAs in postmenopausal osteoporosis

Poly (I:C) and LPS induce distinct immune responses by ovarian stromal fibroblasts

Contact Info

Product

Resources

About