Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti

Magalhães, Tereza; Bergren, Nicholas A.; Bennett, Spencer; Borland, Erin M.; Hartman, Daniel A.; Lymperopoulos, Konstantinos; Sayre, Richard T.; Borlee, Bradley R.; Campbell, Corey L.; Foy, Brian D.; Olson, Kenneth E.; Blair, Carol D.; th, William Black; Kading, Rebekah C.

doi:10.1016/j.ibmb.2019.05.004

Cited by 21 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antiviral RNAi is an intrinsic immune defense mechanism conserved in insects, and flaviviruses can induce vsiRNA production in mosquito cells ( 24 , 25 , 54 ). Although vsiRNA transgenic mosquitoes can effectively block virus transmission ( 55 , 56 ), downregulation of Dicer2 and Ago2 did not enhance ZIKV infection in mosquitoes. Owing to the high laboratory mosquito infection rate (>90%) with ZIKV, we speculate that the viral load in susceptible A. aegypti strains already is operating at maximum capacity.…”

Section: Discussionmentioning

confidence: 98%

Juvenile Hormone-Sensitive Ribosomal Activity Enhances Viral Replication in Aedes aegypti

et al. 2021

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 98%

Juvenile Hormone-Sensitive Ribosomal Activity Enhances Viral Replication in Aedes aegypti

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Aedes aegypti strain Poza Rica (collected in Poza Rica, Mexico, and colonized at Colorado State University (CSU) in 2012; verdadero virus infected) (74) and strains New Orleans (collected from New Orleans, LA, with colony established at CSU in 2005; partitivirus uninfected) and Vergel (collected from Vergel, Mexico and colonized at CSU in 2011; partitivirus uninfected) (75) were used for vertical transmission experiments. Mosquitoes were reared as previously described (76).…”

Section: Methodsmentioning

confidence: 99%

Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission

Cross

Maertens

Dunham

et al. 2020

J Virol

Self Cite

View full text Add to dashboard Cite

Partitiviruses are segmented, multipartite dsRNA viruses that until recently were only known to infect fungi, plants, and protozoans. Metagenomic surveys have revealed that partitivirus-like sequences are also commonly associated with arthropods. One arthropod-associated partitivirus, galbut virus, is common in wild populations of Drosophila melanogaster. To begin to understand the processes that underlie this virus's high global prevalence, we established colonies of wild-caught infected flies. Infection remained at stably high levels over three years, with between 63-100% of individual flies infected. Galbut virus infects fly cells and replicates in tissues throughout infected adults, including reproductive tissues and the gut epithelium. We detected no evidence of horizontal transmission via ingestion but vertical transmission from either infected females or infected males was ∼100% efficient. Vertical transmission of a related partitivirus, verdadero virus, that we discovered in a laboratory colony of Aedes aegypti mosquitoes was similarly efficient. This suggests that efficient biparental vertical transmission may be a feature of at least a subset of insect-infecting partitiviruses. To study the impact of galbut virus infection free from the confounding effect of other viruses, we generated an inbred line of flies with galbut virus as the only detectable virus infection. We were able to transmit infection experimentally via microinjection of homogenate from these galbut-only flies. This sets the stage for experiments to understand the biological impact and possible utility of partitiviruses infecting model organisms and disease vectors. IMPORTANCE Galbut virus is a recently discovered partitivirus that is extraordinarily common in wild populations of the model organism Drosophila melanogaster. Like most viruses discovered through metagenomics, most of the basic biological questions about this virus remain unanswered. We found that galbut virus, along with a closely related partitivirus found in Aedes aegypti mosquitoes, is transmitted from infected females or males to offspring with ∼100% efficiency and can be maintained in laboratory colonies over years. This efficient transmission mechanism likely underlies the successful spread of these viruses through insect populations. We created Drosophila lines that contained galbut virus as the only virus infection and showed that these flies can be used as a source for experimental infections. This provides insight into how arthropod-infecting partitiviruses may be maintained in nature and sets the stage for exploration of their biology and potential utility.

show abstract

“…Aedes aegypti strain Poza Rica (partitivirus infected) (35) and strains New Orleans and Vergel (partitivirus uninfected) (36) were used for vertical transmission experiments. Mosquitoes were reared as previously described (37).…”

Section: Methodsmentioning

confidence: 99%

Partitiviruses infectingDrosophila melanogasterandAedes aegyptiexhibit efficient biparental vertical transmission

Cross

Maertens

Dunham

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

13Partitiviruses are segmented, multipartite dsRNA viruses that until recently were only known to 14 infect fungi, plants, and protozoans. Metagenomic surveys have revealed that partitivirus-like 15 sequences are also commonly associated with arthropods. One arthropod-associated partitivirus, 16 galbut virus, is extraordinarily common in wild populations of Drosophila melanogaster fruit 17 flies. To begin to understand the processes that underlie this virus's high global prevalence, we 18 established colonies of wild-caught infected flies. Infection remained at stably high levels over 19 three years, with between 63-100% of individual flies infected. Galbut virus infects fly cells and 20 replicates in tissues throughout infected adults, including reproductive tissues and the gut 21 epithelium. We detected no evidence of horizontal transmission via ingestion but vertical 22 transmission from either infected females or infected males was ~100% efficient. Vertical 23 transmission of a related partitivirus, verdadero virus, that we discovered in a laboratory colony 24 of Aedes aegypti mosquitoes was similarly efficient. This suggests that efficient biparental 25 vertical transmission may be a feature of at least a subset of insect-infecting partitiviruses. To 26 study the impact of galbut virus infection free from the confounding effect of other viruses, we 27 generated an inbred line of flies with galbut virus as the only detectable virus infection. We were 28 able to transmit infection experimentally via microinjection of homogenate from these galbut-29 only flies. This sets the stage for experiments to understand the biological impact and possible 30 utility of partitiviruses infecting important model organisms and disease vectors.31 32 Keywords: 33 Drosophila melanogaster, Aedes aegypti, galbut virus, verdadero virus, partitivirus, arthropod, 34 vertical transmission, metagenomics, virus discovery, virome. 35 36 Importance 37 38Galbut virus is a recently discovered partitivirus that is extraordinarly common in wild 39 populations of the model organism Drosophila melanogaster. Like most viruses discovered 40 through metagenomics, most of the basic biological questions about this virus remain 41 unanswered. We found that galbut virus, along with a closely related partitivirus found in Aedes 42 aegypti mosquitoes, is transmitted from infected females or males to offspring with ~100% 43 efficiency and can be maintained in laboratory colonies over years. This represents one of the 44 most efficient means of virus transmission described, and likely underlies the successful spread 45 of these viruses through insect populations. We created Drosophila lines that contained galbut 46 virus as the only virus infection and showed that these flies can be used as a source for 47 experimental infections. This provides insight into how arthropod-infecting partitiviruses may be 48 maintained in nature and sets the stage for exploration of their biology and potential utility. 49 50 51 52Metagenomic surveys of wild organisms have...

show abstract

Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti

Cited by 21 publications

References 36 publications

Juvenile Hormone-Sensitive Ribosomal Activity Enhances Viral Replication in Aedes aegypti

Juvenile Hormone-Sensitive Ribosomal Activity Enhances Viral Replication in Aedes aegypti

Partitiviruses Infecting Drosophila melanogaster and Aedes aegypti Exhibit Efficient Biparental Vertical Transmission

Partitiviruses infectingDrosophila melanogasterandAedes aegyptiexhibit efficient biparental vertical transmission

Contact Info

Product

Resources

About