Induction of rho− mutations in yeast Saccharomyces cerevisiae by ethanol

Bandas, E.L.; Захаров, И. А.

doi:10.1016/0027-5107(80)90070-6

Cited by 39 publications

(15 citation statements)

References 9 publications

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“…While ethanol seems to be a strong mutagenic factor in yeast mitochondria (Bandas and Zakharov 1980), it has no genetic effect on the nuclei of yeast cells. It has been suggested that ethanol acts as a mutagen by damaging the mitochondrial membranes of the mitochondrial DNA complex (Bandas and Zakharov 1980). This suggestion is supported by studies on rhomutants induced by a variety of chemical agents, some of which are membrane solvents (such as sodium dodecyl sulphate; Bacila and Horii 1978).…”

Section: Discussionmentioning

confidence: 98%

“…Respiration is directly involved in ethanol tolerance: rho-mutants are more ethanol sensitive with respect to growth (Aguilera and Benltez 1985) and less viable in the presence of ethanol (Bandas and Zakharov 1980) than their isogenic rho + parental strains. The high inhibition of Zymomonas mobilis (an ethanol-producing bacteria) by ethanol is atributed to the inability of this microorganism to shift to an oxidative metabolism (lngram and Buttke 1984).…”

Section: Discussionmentioning

confidence: 99%

“…There must, therefore, be a selective advantage to rho + cells, especially in the presence of ethanol. In fact, rho-mutants of Sacctz cerevisiae are more ethanol sensitive with respect to growth (Brown et al 1984;Aguilera and Benitez 1985) and survival (Bandas and Zakharov 1980) than their rho + parental strains. The fact that exclusively rho § strains are isolated from wines having a high ethanol content (Benltez et al 1983) when the direction of mutation would suggest rho-populations supports this view.…”

Section: Introductionmentioning

confidence: 96%

“…Ethanol itself is a powerful mutagen of the yeast mitochondrial genome -the spontaneous level of rhomutants in Sacch. cerevisiae increases up to 10-fold at 24% (v/v) ethanol (Bandas and Zakharov 1980). Both ethanol-induced and spontaneous rho-mutants cannot regenerate their respiratory ability since such mutants are the results of a deletion in the mitochondrial genome (Zamaroczy et al 1983).…”

Section: Introductionmentioning

confidence: 96%

“…Although only a small percentage of mitochondrial functions are coded for by mitochondrial genes (Myers et al 1985), there are data which suggest that the mitochondrial genome is directly involved in the ethanol tolerance of the cell: the lack of the rho + phenotype leads to a decrease in both growth and viability in ethanol (Bandas and Zakharov 1980;Aguilera and Benitez 1985). In this study, highly ethanol-tolerant wine yeasts (Jim6nez and Benltez 1986) were used to clarify the possible role of the mitochondrial genome in such various functions as ethanol tolerance, thermotolerance, and the mutagenic effects of ethanol and temperature on the mitochondrial genome, as well as their possible interactions.…”

Section: Introductionmentioning

confidence: 96%

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Yeast cell viability under conditions of high temperature and ethanol concentrations depends on the mitochondrial genome

Jiménez

Benı́tez

1988

Curr Genet

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Wine yeasts manifest simultaneously a high tolerance to ethanol, thermotolerance, and a high resistance to the mutagenic effects of ethanol on the mitochondrial genome. The transfer of mitochondria from these strains to laboratory yeasts demonstrate that this genome influences the above parameters, since thermotolerance, ethanol-growth tolerance, and the frequency of rho- mutants were either totally or partially modified in the laboratory recipient strain. When the death rate and the rate of formation of rho- mutants were measured under extreme conditions of inhibitory ethanol concentrations and high temperature, a perfect correlation was found between these parameters, and both of them were dependent on the strain of mitochondrial genome. Thus, the transfer of wine yeast mitochondria leads to a lower death rate, and a simultaneous increase in thermotolerance and ethanol tolerance in the recipient strain. These results demonstrate the role that viability plays under conditions of high temperatures and high ethanol concentrations. The greater stability of the rho+ phenotype shown by the wine yeast mitochondrial genome may be responsible for the increased viability conferred by these mitochondria.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%