Induction of resting B cells to DNA synthesis by soluble monoclonal anti‐immunoglobulin

Julius, Michael; Heusser, Christoph; Hartmann, Klaus-Ulrich

doi:10.1002/eji.1830140816

Cited by 95 publications

(30 citation statements)

References 20 publications

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“…Goat anti-mouse IgM was obtained from Southern Biotechnology, goat anti-mouse IgM F(ab) 2 fragments were from Jackson Immunolaboratories (distributed by Dianova, Hamburg, Germany). Rat anti-CD40 (1C10) 35 and anti IgM (b.7.6) 36 were purified from hybridoma supernatants. IL-4 was from Strathmann (Hamburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis

et al. 2007

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Section: Methodsmentioning

confidence: 99%

The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis

et al. 2007

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“…Ac38 (mouse anti-NPb idiotypic mAb [ll]), RS3.1 (mouse anti-IgMa; [12]) and MB86 (mouse anti-IgMb; [ 131) were purified from culture supernatants or ascites fluids. The mAb b7-6 (rat antimouse IgM; [14]), was a kind gift from Dr. c. Heusser, Ciba-Geigy Ltd, Basel. All antigens were used for coating of ELISA plates at a concentration of 5 pg/ml, except DNA which was used at 10 pglml.…”

Section: Antigens and Antibodiesmentioning

confidence: 99%

Negative selection of multireactive B cell clones in normal adult mice

et al. 1994

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In the absence of intentional immunizations, normal mice produce natural antibodies that react with a variety of self and foreign antigens. We have now addressed the putative physiological selection of such reactivities and some of their clonal characteristics, by analyzing antibodies produced by B cells at different stages of differentiation. Using an antigen-specific spot-enzyme-linked immunosorbent assay (ELISA) with a panel of self and foreign antigens, we found that newly formed B cells, either from adult bone marrow or from newborn spleen, contain the highest frequencies of IgM antibodies with reactivities towards the panel. Resting peripheral B cells show lower frequencies of such antibodies, that are lowest among naturally activated splenic plasma cells. Analyses of monoclonal IgM antibodies derived from lipopolysaccharide-stimulated bone marrow and spleen cell hybridomas in normal mice show that the majority of reactivities scored in spot-ELISA originate from multireactive IgM clones. In Western blots against a large number of self antigens, each multireactive IgM antibody studied shows a unique and specific pattern of reactivity. We conclude that multireactive B cell clones are very frequent in the emergent repertoires of newborns and adults, but are subsequently negatively selected from bone marrow to periphery, and from the available repertoire to that of natural plasma cells. It, thus, seems that multireactivity of natural antibodies is not a positively selected property, but represents the sum of unique multireactive clones that have escaped inactivation or deletion.

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“…Our data with monoclonal rat anti mouse p antibodies revealed that both anti bodies b-7-6 and C-2-23 [8] independent of their isotypes (IgG 1 and IgG 2a) or their fine specificity (mouse cp2 and cp4) suppressed the primary anti-PC response efficiently. Since b-7-6 but not C-2-23 induces thymi dine incorporation of small resting B cells in vitro, one may conclude that their differ ential effect in vitro does not correlate with their effect in vivo on immature neonatal B cells.…”

Section: Discussionmentioning

confidence: 89%

“…(3) Monoclonal rat anti-mouse IgM antibodies: b-7-6 and C-2-23 [8] were derived from a fusion of Sp2/0 cells and spleen cells of TifiRAIF (SPF) rats immunized with a mixture of myeloma proteins (MOPC 104E, TEPC 183, MOPC 21. UPC 10, J 606.…”

Section: Anti-igm Reagentsmentioning

confidence: 99%

Suppression of B Cell Development and Antibody Responses in Mice with Polyclonal Rabbit and Monoclonal Rat Anti-IgM Antibodies

Cerny¹,

Hugin²,

Sutter³

et al. 1985

Pathobiology

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Mice treated from birth with polyclonal, crude or affinity purified rabbit or monoclonal rat anti-mouse IgM antibodies [b-7-6 and C-2-23: Eur. J. Immunol. 14:753–757, 1984] were found to be heavily suppressed with respect to B-cell activities. Crude or affinity purified rabbit or monoclonal rat anti-mouse IgM gave comparable results as follows: (1) serum IgM was below detectable levels; (2) serum IgG was reduced to about 1–3% of normal levels; (3) free anti-IgM was always detectable; (4) IgM and/or Ò¡-light-chain positive cells as well as IgM-secreting cells were absent in various lymphoid organs; (5) the B-cell mitogen lipopolysaccharide was unable to induce proliferative responses; (6) primary antibody responses could not be induced against sheep red blood cells and phosphorylcholine; (7) lymphoid organs were reduced in size and B-cell areas were not populated with lymphocytes; (8) besides a 40% reduction in absolute lymphocyte numbers in the blood, we found increased platelet counts and a 10% eosinophilia in anti-IgM-treated mice.

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Induction of resting B cells to DNA synthesis by soluble monoclonal anti‐immunoglobulin

Cited by 95 publications

References 20 publications

The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis

The novel adaptor protein Swiprosin-1 enhances BCR signals and contributes to BCR-induced apoptosis

Negative selection of multireactive B cell clones in normal adult mice

Suppression of B Cell Development and Antibody Responses in Mice with Polyclonal Rabbit and Monoclonal Rat Anti-IgM Antibodies

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