Induction of rainbow trout MH class I and accessory proteins by viral haemorrhagic septicaemia virus

Sever, Lital; Vo, Nguyen T. K.; Lumsden, John S.; Bols, Niels C.; Dixon, Brian

doi:10.1016/j.molimm.2014.02.001

Cited by 24 publications

(23 citation statements)

References 55 publications

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“…(Sever et al . ). Previously, this anti‐trout MH Iα antibody was shown to cross‐react with a Chinook salmon cell line (CHSE‐214) (Kawano et al .…”

Section: Resultsmentioning

confidence: 97%

“…For rainbow trout, there is no turnover of the MH class I receptors and β 2 m, suggesting that they are produced but not functioning or functioning differently at 4 °C (Sever et al . ). Further studies should investigate whether Arctic charr MH class I receptors turnover at 1–2 °C, given the observation that they grow and are metabolically active at these temperatures, while rainbow trout cells appear less active (Mosser et al .…”

Section: Discussionmentioning

confidence: 97%

“…Furthermore, it seems that rainbow trout cells do not upregulate ERp57 when stimulated much like what was observed in ACBA (Fig a); therefore, future investigations should focus on tapasin, the one MH class I accessory molecule which has been shown to be upregulated in response to viral infection in rainbow trout (Sever et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…When comparing to similar experiments using rainbow trout cells, Sever et al . () was able to show that viral infection at 14 °C can increase the production of MH class I and β 2 m after 14 days, and thus, a longer exposure time to poly I:C may be required for future experiments when analysing components of adaptive immunity. Furthermore, it seems that rainbow trout cells do not upregulate ERp57 when stimulated much like what was observed in ACBA (Fig a); therefore, future investigations should focus on tapasin, the one MH class I accessory molecule which has been shown to be upregulated in response to viral infection in rainbow trout (Sever et al .…”

Section: Discussionmentioning

confidence: 99%

“…; Sever et al . ). However, no cell lines appear to exist for Arctic charr (Bols & Lee ) that would allow exploration of these themes for this species.…”

Section: Introductionmentioning

confidence: 97%

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Development and use of an Arctic charr cell line to study antiviral responses at extremely low temperatures

Semple

et al. 2017

Journal of Fish Diseases

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Arctic charr (Salvelinus alpinus) are the northernmost distributed freshwater fish and can grow at water temperatures as low as 0.2 °C. Other teleost species have impaired immune function at temperatures that Arctic charr thrive in, and thus, charr may maintain immune function at these temperatures. In this study, a fibroblastic cell line, named ACBA, derived from the bulbus arteriosus (BA) of Arctic charr was developed for use in immune studies at various temperatures. ACBA has undergone more than forty passages at 18 °C over 3 years, while showing no signs of senescence-associated β-galactosidase activity and producing nitric oxide. Remarkably, ACBA cells survived and maintained some mitotic activity even at 1 °C for over 3 months. At these low temperatures, ACBA also continued to produce MH class I proteins. After challenge with poly I:C, only antiviral Mx proteins were induced while MH proteins remained constant. When exposed to live viruses, ACBA was shown to permit viral infection and replication of IPNV, VHSV IVa and CSV at 14 °C. Yet at the preferred temperature of 4 °C, only VHSV IVa was shown to replicate within ACBA. This study provides evidence that Arctic charr cells can maintain immune function while also resisting infection with intracellular pathogens at low temperatures.

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“…(Sever et al . ). Previously, this anti‐trout MH Iα antibody was shown to cross‐react with a Chinook salmon cell line (CHSE‐214) (Kawano et al .…”

Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

“…; Sever et al . ). However, no cell lines appear to exist for Arctic charr (Bols & Lee ) that would allow exploration of these themes for this species.…”

Section: Introductionmentioning

confidence: 97%

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