Induction of protective immunity with antibody to herpes simplex virus type 1 glycoprotein H (gH) and analysis of the immune response to gH expressed in recombinant vaccinia virus

Identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek's disease virus and the herpesvirus of turkeys

“…However, expression of the HSV-1 gH in a recombinant vaccinia virus (Blacklaws et al, 1990;Forrester et al, 1991) or COS cell system (Gompels & Minson, 1989) does not result in the wild-type protein being produced. Consequently, vaccinia virus recombinants were unable to elicit neutralizing antibody let alone protection (Blacklaws et al, 1990;Forrester et al, 1991). Additionally, recent work on HSV-1 by Hutchinson et al (1992) demonstrated the need for the presence of another glycoprotein, named gL, for the normal processing and transportion of gH.…”

“…Complement-independent neutralizing antibodies have been raised against gH that inhibit plaque formation in vitro (Buckmaster et al, 1984;Gompels & Minson, 1986;Keller et al, 1987) and protect experimental mice from further virus challenge following passive immunization (Forrester et al, 1991). gH is thus a promising target for inclusion in anti-herpesvirus vaccine development.…”

“…In addition, both these proteins appear to be essential for virus infectivity (Cai et at., 1988;Desai et at., 1988;Forrester et at., 1991). gH was first identified in herpes simplex virus type 1 (HSV-1), following neutralization and immunoprecipitation studies with a series of monoclonal antibodies (MAbs) (Buckmaster et al, 1984) and its sequence was presented by Gompels & Minson (1986) and McGeoch & Davison (1986).…”

Identification of the glycoprotein H gene of murine cytomegalovirus

“…Moreover, these glycoproteins have been shown to be important determinants of virus infectivity (Forrester et al, 1991 ;Misra & Blewett, 1991 ;Cai et al, 1988;Cranage et al, 1988). Monoclonal antibodies to HSV-1 gH and its homologues in VZV, HCMV and EBV are strongly neutralizing, suggesting that gH represents a neutralizing target in all herpesviruses (Forrester et al, 1991 ;Cranage et al, 1988;Oba & Hutt-Fletcher, 1988;Montalvo & Grose, 1986;Rasmussen et al, 1984;Grose et al, 1983).…”

Baculovirus-expressed glycoprotein H of herpes simplex virus type 1 (HSV-1) induces neutralizing antibody and delayed type hypersensitivity responses, but does not protect immunized mice against lethal HSV-1 challenge

“…Passive immunization of mice with anti-gH MAbs LP11 and LP2 can produce clearance of HSV-1 from the ear pinnae (Forrester et al, 1991). In contrast, mice vaccinated with two vaccinia virus recombinants expressing gH are not protected against HSV-1 zosteriform spread or the establishment of latent HSV-1 infection following virus challenge.…”