Induction of protective immunity in chickens immunized with plant-made chimeric Bamboo mosaic virus particles expressing very virulent Infectious bursal disease virus antigen

Chen, Tsung‐Hsien; Chen, Ten-Hong; Hu, Chung-Chi; Liao, Jia-Teh; Lee, Chin‐Wei; Liao, Jiunn‐Wang; Lin, Maw-Yeong; Liu, Hung-Jen; Wang, Min-Ying; Lin, Na-Sheng; Hsu, Yau‐Heiu

doi:10.1016/j.virusres.2012.02.021

Cited by 42 publications

(40 citation statements)

References 46 publications

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“…The observation that the integrated viral constructs has retained the original sequences over 21 days suggested the stability of replicons in the transgenic cell‐suspension cultures. In our previous report, BaMV‐based viral constructs were stable and resulted in successful assembly of CVPs in whole‐plant systems up to three serial passages (Chen et al ., ).…”

Section: Discussionmentioning

confidence: 96%

A transgenic plant cell‐suspension system for expression of epitopes on chimeric Bamboo mosaic virus particles

Muthamilselvan

Lee

Cho

et al. 2015

Plant Biotechnology Journal

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SummaryWe describe a novel strategy to produce vaccine antigens using a plant cell-suspension culture system in lieu of the conventional bacterial or animal cell-culture systems. We generated transgenic cell-suspension cultures from Nicotiana benthamiana leaves carrying wild-type or chimeric Bamboo mosaic virus (BaMV) expression constructs encoding the viral protein 1 (VP1) epitope of foot-and-mouth disease virus (FMDV). Antigens accumulated to high levels in BdT38 and BdT19 transgenic cell lines co-expressing silencing suppressor protein P38 or P19. BaMV chimeric virus particles (CVPs) were subsequently purified from the respective cell lines (1.5 and 2.1 mg CVPs/20 g fresh weight of suspended biomass, respectively), and the resulting CVPs displayed VP1 epitope on the surfaces. Guinea pigs vaccinated with purified CVPs produced humoral antibodies. This study represents an important advance in the large-scale production of immunopeptide vaccines in a cost-effective manner using a plant cell-suspension culture system.

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Section: Discussionmentioning

confidence: 96%

A transgenic plant cell‐suspension system for expression of epitopes on chimeric Bamboo mosaic virus particles

Muthamilselvan

Lee

Cho

et al. 2015

Plant Biotechnology Journal

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“…It is well accepted that the N-terminus of PapMV and potexvirus CP is exposed at the surface of the virus particle [2,8,21,22]. The recently published 3D structure of PapMV CP revealed that the N-terminus is involved in the interaction between two CP subunits in the virus particle [2], and that the 12 N-terminal residues upstream of F13 are directly exposed on the surface [14].…”

Section: Resultsmentioning

confidence: 99%

Engineering of papaya mosaic virus (PapMV) nanoparticles with a CTL epitope derived from influenza NP

2013

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BackgroundThe ever-present threat of infectious disease, e.g. influenza pandemics, and the increasing need for new and effective treatments in immunotherapy are the driving forces that motivate research into new and innovative vaccine platforms. Ideally, such platforms should trigger an efficient CTL response, be safe, and easy to manufacture. We recently developed a novel nanoparticle adjuvant comprised of papaya mosaic virus (PapMV) coat protein (CP) assembled around an RNA. The PapMV nanoparticle is an efficient vaccine platform in which the peptide antigen is fused to the C-terminus of the PapMV CP, leading to nanoparticles presenting surface-exposed epitope. The fusion stabilizes the epitope and improves its immunogenicity. We found recently that C-terminal fusions are not always efficient, depending on the nature of the peptide fused to the platform.ResultsWe chose a CTL epitope derived from the nucleocapsid (NP) of influenza virus (NP147-155) for this proof-of-concept demonstration. Recombinant nanoparticles harbouring a fusion at the N-terminus were more efficient in triggering a CTL response. Efficacy appeared to be linked to the stability of the nanoparticles at 37°C. We also showed that discs—smaller than nanoparticles—made of 20 subunits of PapMV CP are less efficient for induction of a CTL response in mice, revealing that assembly of the recombinant PapMV CP into nanoparticles is crucial to triggering an efficient CTL response.ConclusionThe point of fusion on the PapMV vaccine platform is critical to triggering an efficient CTL response. Efficacy is linked to nanoparticle stability; nanoparticles must be stable at 37°C but remain susceptible to cellular proteases to ensure efficient processing of the CTL epitope by cells of the immune system. The results of this study improve our understanding of the PapMV vaccine platform, which will facilitate the design of efficient vaccines to various infectious threats.

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“…In 2013, Taghavian reported that tobacco plant produced via transgenesis mediated by Cauliflower mosaic virus for production of VP2 antigen was as effective as commercial vaccines, in producing immunity in mice. Chen et al, 2012 first reported the process describing the production of an IBDV VP2 epitope vaccine in Chenopodium quinoa using plant virus epitope (Bamboo mosaic virus) presentation system. In 2013, Gomez et al, through agroinfiltration of Nicotiana benthamiana for expression of VP2 protein could obtain 1% of total soluble protein as expressed protein.…”

Section: Plant Based Edible Vaccines Against Poultry Diseases Infectimentioning

confidence: 99%

Plant Based Edible Vaccines against Poultry Diseases: a Review

Aswathi¹

2014

Adv. Anim. Vet. Sci.

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Induction of protective immunity in chickens immunized with plant-made chimeric Bamboo mosaic virus particles expressing very virulent Infectious bursal disease virus antigen

Cited by 42 publications

References 46 publications

A transgenic plant cell‐suspension system for expression of epitopes on chimeric Bamboo mosaic virus particles

A transgenic plant cell‐suspension system for expression of epitopes on chimeric Bamboo mosaic virus particles

Engineering of papaya mosaic virus (PapMV) nanoparticles with a CTL epitope derived from influenza NP

Plant Based Edible Vaccines against Poultry Diseases: a Review

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