Induction of Protective Immunity by Primed B‐1 Cells in <i>Toxoplasma gondii</i>‐Infected B Cell‐Deficient Mice

Chen, Mei; Mun, Hye-Seong; Li, Piao; Aosai, Fumie; Norose, Kazumi; Mohamed, Rabie M.; Belal, Usama S.; Fang, Hao; Ahmed, Ashour M.; Kang, Hyun-Kyu; Matsuzaki, Goro; Kitamura, Daisuke; Yano, Akihiko

doi:10.1111/j.1348-0421.2003.tb03460.x

Cited by 18 publications

(11 citation statements)

References 32 publications

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“…Primed B cells of this lymphocyte population have been shown to promote protective immunity against the apicomplexan parasite Toxoplasma gondii. 48 Also, and similarly to our finding in NcT‐infected mice, an expansion of splenic B‐1 cells, associated with host protection, has previously been described in Plasmodium chabaudi ‐infected mice 49 …”

Section: Discussionsupporting

confidence: 85%

Characterization of the B‐cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites

et al. 2005

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SummaryActivation of B cells occurring in hosts infected with protozoan parasites has been implicated either in protective or parasite-evasion immune-mediated mechanisms. Intraperitoneal inoculation of Neospora caninum tachyzoites into BALB/c mice induces an acute response characterized by a rapid increase in the numbers of CD69-expressing peritoneal and splenic B cells. This early B-cell stimulatory effect preceded an increase in the numbers of total and immunoglobulin-secreting splenic B cells and a rise in serum levels of N. caninum-specific immunoglobulins, predominantly of the immunoglobulin G2a (IgG2a) and IgM isotypes. Increased numbers of B cells expressing the costimulatory molecules CD80 and CD86 were also observed in the N. caninum-infected mice. The B-cell stimulatory effect observed in mice challenged with N. caninum tachyzoites was reduced in mice challenged with c-irradiated parasites. Contrasting with the peripheral B-cell expansion, a depletion of B-lineage cells was observed in the bone-marrow of the N. caninum-infected mice. Intradermal immunization of BALB/c mice with diverse N. caninum antigenic preparations although inducing the production of parasite-specific antibodies nevertheless impaired interferon-c (IFN-c) mRNA expression and caused lethal susceptibility to infection in mice inoculated with a nonlethal parasitic inoculum. This increased susceptibility to N. caninum was not observed in naïve mice passively transferred with anti-N. caninum antibodies. Taken together, these results show that N. caninum induces in BALB/c mice a parasite-specific, non-polyclonal, B-cell response, reinforce previous observations made by others showing that immunization with N. caninum whole structural antigens increases susceptibility to murine neosporosis and further stress the role of IFN-c in the host protective immune mechanisms against this parasite.

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Section: Discussionsupporting

confidence: 85%

Characterization of the B‐cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites

et al. 2005

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“…Upon LPS stimulation both in vitro and in vivo , B-1 B cells down-modulated surface integrins including alpha1, alpha6 and beta1 and CD9, a surface receptor important for adhesion to local matrix- changes that permitted more rapid movement of cells in response to chemokines. Other in vivo studies using a range of infectious models including S. pneumoniae (94), Borrelia hermsii (95), Cryptococcus neoformans (96), influenza virus (97) and Toxoplasmagondii (98) have shown the requirement of B-1 B cells during the induced immune response. Many of these pathogens probably involve TLR signals for activation of B-1 B cells although a direct proof is often missing.…”

Section: Distinct Functions Of B Cell Subpopulations In Response Tmentioning

confidence: 99%

Differential impact of Toll-like receptor signaling on distinct B cell subpopulations

Meyer‐Bahlburg

Rawlings

2012

Front Biosci

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B cells exhibit a range of functional responses following TLR engagement including immunoglobulin and cytokine production, proliferation, antigen presentation and migration. However, B cell intrinsic TLR responses appear to be precisely programmed based upon the developmental stage of the cell. B cell subpopulations classified as innate immune cells including marginal zone and B-1 B cells exhibit robust responses to TLR stimulation. In contrast, activation of other B cell subsets is constrained via a variety of developmentally regulated events. In this review we provide an overview of TLR responses in murine and human B cells and specifically highlight patterns of TLR expression and developmentally regulated functional responses.

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“…T. gondii infection in pregnant women can be transmitted to the fetus and may cause severe injuries to the fetus, such as mental retardation, blindness, epilepsy and death (Jones et al 2001). The administration of antisera to T. gondii effects a reduction of mortality in animals (Kang et al 2000;Chen et al 2003). Antibodies are the body's first-line of defence, and they act on the extracellular tachyzoites released following infected cell lysis.…”

Section: Introductionmentioning

confidence: 99%

FlowCytomix analysis forToxoplasma gondiiinfection in pregnant women in central Taiwan

Chou

Lin²,

Chen³

et al. 2011

Journal of Obstetrics and Gynaecology

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The study was to determine the seroprevalence of toxoplasmosis in the sera of pregnant women in central Taiwan and to investigate the levels of cytokine in the sera of pregnant women with Toxoplasma gondii infection. The 220 blood samples were collected from pregnant women. The haematological parameters of peripheral blood were analysed by a haematology analyser. Serum samples of the pregnant women were analysed by a commercially available anti-T. gondii IgM/IgG antibody enzyme-linked immunosorbent assay (ELISA) kit and FlowCytomix assays. Six (2.7%) of the sera samples had IgM anti-T. gondii antibodies, and twenty (9.1%) had T. gondii IgG seropositive. All six IgM seropositive samples had low IgG avidity, indicative of acute infection. Total white blood cells and eosinophils were statistically significantly increased (p<0.05) in pregnant women with T. gondii infection, as compared with healthy pregnant women. Th1 cytokines IFN-γ, IL-1β, IL-2 and IL-12 p70, and Th2 cytokines IL-10 in pregnant women with T. gondii IgM/IgG seropositive were significantly increased (p<0.05), as compared with healthy pregnant women. These results showed that both of Th1 and Th2 cytokines play an important role in the toxoplasmosis of pregnant women.

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Induction of Protective Immunity by Primed B‐1 Cells in Toxoplasma gondii‐Infected B Cell‐Deficient Mice

Cited by 18 publications

References 32 publications

Characterization of the B‐cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites

Characterization of the B‐cell immune response elicited in BALB/c mice challenged with Neospora caninum tachyzoites

Differential impact of Toll-like receptor signaling on distinct B cell subpopulations

FlowCytomix analysis forToxoplasma gondiiinfection in pregnant women in central Taiwan

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