Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene

Desolme, Benoît; Mévélec, Marie-Noëlle; Buzoni‐Gatel, Dominique; Bout, Daniel

doi:10.1016/s0264-410x(00)00035-9

Cited by 82 publications

(55 citation statements)

References 50 publications

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“…Whole coding sequence of GRA4 has been tried as a DNA vaccine, which results in a 62% survival of susceptible C57BL/6 infected mice [44]. Vaccine efficacy of recombinant GRA4 (rGRA4) and ROP2 (rRPO2) proteins and a mix of both combined with alum is evaluated in C57BL/6 and C3H mice [45].…”

Section: Gra4mentioning

confidence: 99%

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

Nam

2009

Korean J Parasitol

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Abstract:The dense granule of Toxoplasma gondii is a secretory vesicular organelle of which the proteins participate in the modification of the parasitophorous vacuole (PV) and PV membrane for the maintenance of intracellular parasitism in almost all nucleated host cells. In this review, the archives on the research of GRA proteins are reviewed on the foci of finding GRA proteins, characterizing molecular aspects, usefulness in diagnostic antigen, and vaccine trials in addition to some functions in host-parasite interactions.

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Section: Gra4mentioning

confidence: 99%

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

Nam

2009

Korean J Parasitol

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“…Although DNA immunization can elicit some degree of protective immunity, the activation of specific CD8 T cells has been poorly or not at all characterized (Nielsen et al 1999, Angus et al 2000, Vercammen et al 2000, Desolme et al 2000. Whether subunit vaccines may induce protective CD8 T cells specific for these antigen remains to be further characterized in the future.…”

Section: Toxoplasma Gondii and Leishmania Spmentioning

confidence: 99%

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Rodrigues

Boscardin

Vasconcelos

et al. 2003

An. Acad. Bras. Ciênc.

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Obligatory intracellular parasites such as Plasmodium sp, Trypanosoma cruzi, Toxoplasma gondii and Leishmania sp are responsible for the infection of hundreds of millions of individuals every year. These parasites can deliver antigens to the host cell cytoplasm that are presented through MHC class I molecules to protective CD8 T cells. The in vivo priming conditions of specific CD8 T cells during natural infection are largely unknown and remain as an area that has been poorly explored. The antiparasitic mechanisms mediated by CD8 T cells include both interferon-γ -dependent and -independent pathways. The fact that CD8 T cells are potent inhibitors of parasitic development prompted many investigators to explore whether induction of these T cells can be a feasible strategy for the development of effective subunit vaccines against these parasitic diseases. Studies performed on experimental models supported the hypothesis that CD8 T cells induced by recombinant viral vectors or DNA vaccines could serve as the basis for human vaccination. Regimens of immunization consisting of two different vectors (heterologous prime-boost) are much more efficient in terms of expansion of protective CD8 T lymphocytes than immunization with a single vector. The results obtained using experimental models have led to clinical vaccination trials that are currently underway.

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“…Numerous studies have evaluated responses to vaccines based on protein or DNA components of T. gondii with various degrees of success (1,2,4,5,9,11,22,24,25,28,36,37,40,44,46). Virulent parasite strains, as well as attenuated T. gondii strains, have been paramount in dissecting the immunobiology of host response in regard to understanding adaptive immune responses that may be helpful in vaccine design.…”

mentioning

confidence: 99%

Long-Term Immunity to Lethal Acute or Chronic Type IIToxoplasma gondiiInfection Is Effectively Induced in Genetically Susceptible C57BL/6 Mice by Immunization with an Attenuated Type I Vaccine Strain

Gigley¹,

Fox²,

Bzik³

2009

Infect Immun

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C57BL/6 (B6) mice are genetically highly susceptible to chronic type II Toxoplasma gondii infections that invariably cause lethal toxoplasmic encephalitis. We examined the ability of an attenuated type I vaccine strain to elicit long-term immunity to lethal acute or chronic type II infections in susceptible B6 mice. Mice immunized with the type I cps1-1 vaccine strain were not susceptible to a lethal (100-cyst) challenge with the type II strain ME49. Immunized mice challenged with 10 ME49 cysts exhibited significant reductions in brain cyst and parasite burdens compared to naive mice, regardless of the route of challenge infection. Remarkably, cps1-1 strain-immunized B6 mice chronically infected with ME49 survived for at least 12 months without succumbing to the chronic infection. Potent immunity to type II challenge infections persisted for at least 10 months after vaccination. While the cps1-1 strain-elicited immunity did not prevent the establishment of a chronic infection or clear established brain cysts, cps1-1 strain-elicited CD8؉ immune T cells significantly inhibited recrudescence of brain cysts during chronic ME49 infection. In addition, we show that uracil starvation of the cps1-1 strain induces early markers of bradyzoite differentiation. Collectively, these results suggest that more effective immune control of chronic type II infection in the genetically susceptible B6 background is established by vaccination with the nonreplicating type I uracil auxotroph cps1-1 strain.

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Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii GRA4 gene

Cited by 82 publications

References 50 publications

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane

Importance of CD8 T cell-mediated immune response during intracellular parasitic infections and its implications for the development of effective vaccines

Long-Term Immunity to Lethal Acute or Chronic Type IIToxoplasma gondiiInfection Is Effectively Induced in Genetically Susceptible C57BL/6 Mice by Immunization with an Attenuated Type I Vaccine Strain

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