Induction of Protective Immunity Against Experimental Amebic Abscess of the Liver (EAAL) Using the Monocyte Locomotion Inhibitory Factor (MLIF) Produced by Entamoeba histolytica

López-Osuna, M; Cárdenas, Guadalupe; Isario, José; Arellano, Jorge; Fernández-Díez, Jorge; Isibasí, Armando; Kretschmer, R

doi:10.1016/s0188-4409(00)00168-5

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“…8 weeks), and apparently specific state of protection, as it did not prevent the induction of Listeria monocytogenes abscesses of the liver (ELAL). Previous studies that had revealed a 100% EAAL protection using Freund's adjuvant with or without MLIF, were therefore probably the result of an intense and persistent state of adjuvant‐activated innate immunity that overwhelmed whatever adaptive immunity present (18,19). A pre‐excited state of innate immunity – an improbable event using a MAPS device anyhow – thus appears unlikely as the explanation for the EAAL protection using MLIF–MAPS immunization.…”

Section: Discussionmentioning

confidence: 99%

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Immunization with a tetramer derivative of an anti‐inflammatory pentapeptide produced by Entamoeba histolytica protects gerbils (Meriones unguiculatus) against experimental amoebic abscess of the liver

Giménez-Scherer

Cárdenas

López-Osuna

et al. 2004

Parasite Immunology

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Axenically grown Entamoeba histolytica produces a pentapeptide (Met-Gln-Cys-Asn-Ser) with several anti-inflammatory properties, including the inhibition of human monocyte locomotion (Monocyte Locomotion Inhibitory Factor (MLIF)). A construct displays the same effects as the native material. It remains to be seen if MLIF is used, or even produced in vivo by the tissue-invading parasite. If MLIF were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantage and prevent lesions. KLH-linked MLIF mixed with Freund's adjuvant was too aggressive an immunizing material to answer this question. However, immunization with a tetramer of MLIF (but not a scrambled version of MLIF) around a lysine core (MLIF-MAPS), that displays increased antigenicity, yet lacks excessive innate immunity activation, completely protects gerbils against amoebic abscess of the liver caused by the intraportal injection of virulent E. histolytica. Liver abscesses caused by Listeria monocytogenes were not prevented. Invasive E. histolytica may produce the parent protein of MLIF in vivo, and if appropriately cleaved, it may play a role in invasive amoebiasis. MLIF may join new vaccination strategies against amoebiasis.

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Section: Discussionmentioning

confidence: 99%

“…This led to 100% protection, but so did Freund's adjuvant injections alone. No humoral antibodies to MLIF were found, and delayed skin hypersensitivity was elicited only to the carrier (KLH) (18,19). Instead of such aggressive adjuvant preparations (i.e.…”

Section: Introductionmentioning

confidence: 99%