Induction of potato systemic resistance against the potato virus Y (PVYNTN), using crude filtrates of Streptomyces spp. under greenhouse conditions

Abstract

The potato virus Y (PVY) is considered one of the most important viruses in terms of both economical and biological impact. Potential of using three different Streptomyces spp. crude filtrates (SCF) to induce of resistance against tuber necrotic strain of potato virus Y (PVY NTN) in four potato cultivars namely, Spunta, Nicola, Selatar, and Diamant, were evaluated. Foliar spraying of Streptomyces netropsis DSM 40093 (SCF7), S. ambofaciens (SCF11) and S. actuosus (SCF20), applied separately on potato cultivated in pots, showed various inhibitory activities against PVY NTN infection in all the potato cultivars. SCF20 was the most effective one, as expressed by (100%) reduction of apical necrosis symptoms on potato cv. Spunta, Nicola, and Diamant leaves under greenhouse conditions. Application was done 7 days before virus inoculation with PVY NTN (DBVI) and 7 days after inoculation (DAVI). The concentration of PVY NTN in treatments was significantly reduced than in the untreated PVY NTN infected plants as detected by DAS-ELISA (expressed by decreasing in OD value). Expression of PR-1b gene was detected 21 (DAVI) in all SCF11-treated cultivars but was not expressed in healthy and infected control plants. Gas chromatography-mass spectrometry (GC-MS) analysis of S. actuosus (SCF20) ethyl acetate extract revealed the majority of fatty acids (FAs) and their derivatives, Citroflex A, Hexanoic acid, anhydride, Hexadecanoic acid, Hexadecanoic acid, ethyl ester, and Diisooctyl phthalate which suggested to act as elicitor molecules for induction of systemic acquired resistance (SAR). Overall, the application of culture filtrates of Streptomyces has the potentiality to be used as inducer against PVY NTN infection.

Bacillus licheniformis strain POT1 mediated polyphenol biosynthetic pathways genes activation and systemic resistance in potato plants against Alfalfa mosaic virus

“…In this context, the foliar application of Streptomyces spp. CF exhibited a significant reduction of PVY in potato 39 . Thus, POT1 activates induced systemic resistance (ISR) of potato plants against AMV infection.…”

Streptomyces sp. JCK-6131 Protects Plants Against Bacterial and Fungal Diseases via Two Mechanisms

“…They can directly trigger or produce elicitors that can induce systemic resistance in the plant against pathogens ( Dias et al, 2017 ; Abbasi et al, 2019 ; Vergnes et al, 2020 ). Nasr-Eldin et al (2019) reported that the foliar spraying of the fermentation filtrates of Streptomyces spp. showed effective protection against potato-virus Y in all potato cultivars.…”

Fermentation Strategies to Improve Soil Bio-Inoculant Production and Quality

“…Such final fermentation products, which resulted after medium optimization and fermentation process parameters (pH, temperature, air supply and agitation) are rich in biomass and high spore numbers, but also in metabolites important for plant growth and health and can be applied directly with double effect on plants. In recent years, cell-free fermentation liquids with strong phyto-stimulating and biocontrol properties are attracting a growing interest because their action does not depend on the soil micro-and macro-biota and the typical stress factors affecting living-cell biofertilizers [10,[47][48][49][50][51]. Optimization of the fermentation medium may include introduction of additional components in order to increase the system productivity or control metabolite activity.…”