1989
DOI: 10.1159/000235057
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Induction of Polyclonal and Monoclonal Antibodies with a Single Allergen Separated from Birch Pollen Extract by SDS-PAGE and Nitrocellulose Blots

Abstract: A Gold staining allergenic band, Bet v 1, was excised from a nitrocellulose blot following SDS-PAGE of birch pollen extract. This was used to raise a polyclonal rabbit antiserum and monoclonal mouse antibodies specific only for the Bet v 1. The method offers practical advantages for the production of antibodies to individual allergens in complex extract mixtures, without laborious purification methods.

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“…A number of mAb have been produced already against grass [12][13][14][15], ragweed [16], cat and dog [17] and mite [18] for this purpose. Recently, anti-Bet v I mAb have been also produced and used to purify the molecule [2,19]. In this work, several anti-Bet v I mAb were produced and characterized: they were used to define the epitopic structure of the molecule and identify the allergenic epitopes.…”
Section: Introductionmentioning
confidence: 99%
“…A number of mAb have been produced already against grass [12][13][14][15], ragweed [16], cat and dog [17] and mite [18] for this purpose. Recently, anti-Bet v I mAb have been also produced and used to purify the molecule [2,19]. In this work, several anti-Bet v I mAb were produced and characterized: they were used to define the epitopic structure of the molecule and identify the allergenic epitopes.…”
Section: Introductionmentioning
confidence: 99%