Induction of poly(ADP-ribosyl)ation and DNA damage in human peripheral lymphocytes after treatment with (−)-epigallocatechin-gallate

Bertram, Barbara; Bollow, Ursula; Rajaee-Behbahani, Nahid; Bürkle, Alexander; Schmezer, Peter

doi:10.1016/s1383-5718(02)00245-0

Cited by 39 publications

(34 citation statements)

References 30 publications

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“…Some researchers showed that tannic acid antioxidant/pro-oxidant behaviour is a dose-dependent manner because an increase in non-toxic concentrations of tannic acid caused a slight non-significant increase of O 2 -, NO and malondialdehyde (MDA). It seems that low concentrations of tannic acid are beneficial for cells, as scavengers for ROS intermediates, causing prevention of forming ROS and the concomitant enhancement of lipid peroxidation (Salminen et al, 2001;Bertram et al, 2003;Perron and Brumaghim, 2009). …”

Section: Effects Of Tannic Acid On Broilersmentioning

confidence: 99%

Effects of Tannic Acid on Performance and Fatty Acid Composition of Breast Muscle in Broiler Chickens Under Heat Stress

Ebrahim

Liang

Jahromi

et al. 2015

Italian Journal of Animal Science

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One hundred twenty day-old broiler chicks (Cobb 500) were randomly assigned into 4 treatment groups to investigate the effects of tannic acid supplementation (TA) on fatty acid composition in breast muscle of broilers under chronic heat exposure conditions. Five pen replicates of 6 chicks each were assigned to each of the following 4 dietary treatments: i) basal diet containing no TA at 25°C (CL); ii) basal diet containing no TA at 35°C (CH); iii) basal diet supplemented with 1% TA at 25°C (TL); and iv) basal diet supplemented with 1% TA at 35°C (TH). At the end of the 5-week experiment, breast muscle samples were collected to examine the fatty acid composition. Results showed that temperature, TA and their interaction effect significantly decreased body weight gain and feed intake. In addition, feed conversion ratio (FCR) significantly increased under high temperature, and addition of TA under high temperature did not improve the FCR. The effects of temperature, TA and their interaction on the saturated and unsaturated fatty acids were not significant (P>0.05). However, monounsaturated fatty acids significantly reduced by adding TA to the diet. Generally, TA improved the fatty acid profile of breast muscle of broilers under heat stress in comparison to the heat stressed chickens, which did not receive TA. Hence, it seems that dietary TA supplementation can be applied as a biological antioxidant for poultry nutrition in hot climatic conditions.

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Section: Effects Of Tannic Acid On Broilersmentioning

confidence: 99%

Effects of Tannic Acid on Performance and Fatty Acid Composition of Breast Muscle in Broiler Chickens Under Heat Stress

Ebrahim

Liang

Jahromi

et al. 2015

Italian Journal of Animal Science

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“…Catechin inhibits the activities of various proteins attached to DNA, such as DNA polymerase, cAMP-response element-binding protein, DNA methyltransferase, and DNA topoisomerase (21)(22)(23)(24)(25)(26). These reactions are likely affected by EGCG binding to DNA and RNA.…”

Section: Discussionmentioning

confidence: 99%

“…Catechins induce apoptosis in cells and sometimes cause DNA damage (21)(22)(23)(24)(25)(26), phenomena that are due to oxidative stress. However, this might be triggered by the direct binding of catechins to DNA.…”

Section: Discussionmentioning

confidence: 99%

DNA and RNA as New Binding Targets of Green Tea Catechins

Kuzuhara

Sei

Yamaguchi

et al. 2006

Journal of Biological Chemistry

133

111

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The significance of catechins, the main constituent of green tea, is being increasingly recognized with regard to cancer prevention. Catechins have been studied for interactions with various proteins, but the mechanisms of the various catechins are not yet elucidated. Based on our previous observation that nucleic acids extracted from catechintreated cells are colored, we studied whether catechins directly interact with nucleic acids using surface plasmon resonance assay (Biacore) and cold spray ionization-mass spectrometry. These two methods clearly showed that (؊)-epigallocatechin gallate (EGCG) binds to both DNA and RNA molecules: the Biacore assay indicated that four catechins bound to DNA oligomers, and cold spray ionization-mass spectrometry analysis showed one to three EGCG molecules bound to single strand 18 mers of DNA and RNA. Moreover, one or two molecules of EGCG bound to double-stranded (AG-CT) oligomers of various nucleotide lengths. These results suggest that multiple binding sites of EGCG are present in DNA and RNA oligomers. Double-stranded DNA (dsDNA) oligomers were detected only as EGCG-bound forms at high temperature, whereas at low temperature both the free and bound forms were detected, suggesting that EGCG protects dsDNA oligomers from dsDNA melting to single-stranded DNA. Because both galloyl and catechol groups of EGCG are essential for DNA binding, both groups seem to hold strands of DNA via their branching structure. These findings reveal for the first time the link between catechins and polynucleotides and will intensify our understanding of the effects of catechins on DNA in terms of cancer prevention.Green tea is an acknowledged cancer preventive in Japan (1-7). Most of the active principles are assumed to be green tea catechins because they show various cancer-preventive activities in vitro in cell culture and in vivo, including anti-oxidant, anti-cancer, and antimutagenic activities (1-7). (Ϫ)-Epigallocatechin gallate (EGCG), 2 (Ϫ)-epigallocatechin (EGC), (Ϫ)-epicatechin gallate (ECG), (Ϫ)-epicatechin (EC), (Ϫ)-gallocatechin gallate (GCG), and (ϩ)-catechin are the major components of green tea polyphenols (8), and EGCG is the main constituent of green tea (9). Since we first reported in 1987 that topical applications of EGCG inhibited tumor promotion with teleocidin of the 12-O-tetradecanoylphorbol-13-acetate types and okadaic acid on mouse skin in two-stage carcinogenesis experiments (10, 11), we have studied the mechanisms of action of green tea catechins in human cancer cell lines (12-18). During our study of EGCG, we often observed that nucleic acids extracted from EGCGtreated human cancer cells were catechin colored, suggesting that EGCG binds to DNA and RNA molecules in cells. Our speculation was strengthened by results showing that 3 H-EGCG was found in nuclei of human lung cancer cell line PC-9 1 h after treatment (13). Although EGCG apparently protects against DNA damage induced by free radicals, ionization, and ultraviolet radiation, as well as DNA methylation (5,19,20),...

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“…EGCG는 녹차 catechins의 대부분을 차지하며 전체 녹차 내 총 폴리페놀의 약 40%에 해당된다 (3). EGCG는 녹차 catechins 중 가장 잘 알려진 활성 물질이지만 EGCG는 생체이용률이 낮고 중성 이나 알칼리성 용액에 불안정성하다는 제한점이 있으며(4) 특히 EGCG은 DNA 손상을 야기하며 인체 세포주에서는 염색체이상(chromosome aberration)과 자매염색분체교환 (sister chromatid exchanges)을 유도하고 (5,6), 동물실험에 서 대장암 유발을 강화함이 보고되기도 하였다 (7,8 하게 활용되고 있다 (20 …”

Section: 명하는 주된 성분이다 녹차의 Catechins에는 (-)-Epicatechin(ec) (-)-Epicatunclassified

Optimal Reaction Conditions and Radical Scavenging Activities for the Bioconversion of Green Tea Using Tannase

Hong¹,

Yeon²,

Jung³

et al. 2011

Journal of the Korean Society of Food Science and Nutrition

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In this study, we optimized the reaction conditions for the bioconversion of green tea using tannase, and to evaluate its radical scavenging activities. Tea catechins such as (-)-epigallocatechin gallate (EGCG) or (-)-epicatechin gallate (ECG) were hydrolyzed by tannase to produce (-)-epigallocatechin (EGC) or (-)-epicatechin (EC), respectively, and a common product, gallic acid. The bioconversion of tea catechins by tannase was increased as enzyme concentration, substrate concentration and incubation time for enzyme dose. The results indicated the optimum reaction conditions for tannase were tannase 30 U/mL (enzyme concentration) on 1% green tea (substrate concentration) for 1 hr (incubation time for enzyme). Tannase enhanced the radical-scavenging properties of green tea; the 2,2-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals scavenging abilities were significantly (p<0.001) greater for the tannase-treated green tea extract compared to the untreated green tea extract. It is reported that ECG has the greatest antioxidant activity among the catechins in green tea, and the release of gallic acid is considered to be beneficial because of its significant antioxidant potency. The results of this study suggest that the tannase-treated green tea increases antioxidant activities under optimum reaction conditions.

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Induction of poly(ADP-ribosyl)ation and DNA damage in human peripheral lymphocytes after treatment with (−)-epigallocatechin-gallate

Cited by 39 publications

References 30 publications

Effects of Tannic Acid on Performance and Fatty Acid Composition of Breast Muscle in Broiler Chickens Under Heat Stress

Effects of Tannic Acid on Performance and Fatty Acid Composition of Breast Muscle in Broiler Chickens Under Heat Stress

DNA and RNA as New Binding Targets of Green Tea Catechins

Optimal Reaction Conditions and Radical Scavenging Activities for the Bioconversion of Green Tea Using Tannase

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