Induction of Plant Defense Enzymes and Phenolics by Treatment With Plant Growth–Promoting Rhizobacteria Serratia marcescens NBRI1213

Abstract

In greenhouse experiments, plant growth-promoting rhizobacteria (PGPR) Serratia marcescens NBRI1213 was evaluated for plant growth promotion and biologic control of foot and root rot of betelvine caused by Phytophthora nicotianae. Bacterization of betelvine (Piper betle L.) cuttings with S. marcescens NBRI1213 induced phenylalanine ammonia-lyase, peroxidase, and polyphenoloxidase activities in leaf and root. Qualitative and quantitative estimation of phenolic compounds was done through high-performance liquid chromatography (HPLC) in leaf and root of betelvine after treatment with S. marcescens NBRI1213 and infection by P. nicotianae. Major phenolics detected were gallic, protocatechuic, chlorogenic, caffeic, ferulic, and ellagic acids by comparison of their retention time with standards through HPLC. In all of the treated plants, synthesis of phenolic compounds was enhanced compared with control. Maximum accumulation of phenolics was increased in S. marcescens NBRI1213-treated plants infected with P. nicotianae. In a greenhouse test, bacterization using S. marcescens NBRI1213 decreased the number of diseased plants compared with nonbacterized controls. There were significant growth increases in shoot length, shoot dry weight, root length, and root dry weight, averaging 81%, 68%, 152%, and 290%, respectively, greater than untreated controls. This is the first report of PGPR-mediated induction of phenolics for biologic control and their probable role in protecting betelvine against P. nicotianae, an important soil-borne phytopathogenic fungus.

Influence of Serratia marcescens TRS-1 on growth promotion and induction of resistance in Camellia sinensis against Fomes lamaoensis

“…As tea plants are cultivated for their leaves from which the beverage is made, an increase in leaf number is considered to be of importance to the tea industry. In an early study, S. marcescens NBR11213 was reported to induce plant growth promotion and biological control of foot and root rot of betelvine caused by Phytophthora nicotianeae (Lavania et al 2006). Guo et al (2004) also reported that three strains of PGPRs Á Serratia sp.…”

Azoxystrobin induces lignification-related enzymes and phenolics in rice (Oryza sativaL.) against blast pathogen (Pyricularia grisea)

“…Hence enhanced lignification and phenolics are the most important molecular events during fungal evasion of host defense mechanisms (Walters et al 2005). The inductions were achieved by using excellent elicitors like chemicals (Silué et al 2002), biocontrol agents (Lavania et al 2006), plant extract (Guleria & Kumar 2006) and fungicides (Molina et al 1998). Normally, the induced lignification is reflected by enhanced enzyme activities and phenol.…”

Aktivitas Enzim Peroksidase Bawang Merah Yang Diintroduksi Dengan Bakteri Endofit Dan Tahan Terhadap Penyakit Hawar Daun Bakteri (Xanthomonas Axonopodis Pv. Allii)

“…Serratia marcesscen 90-166 mengurangi serangan Cucumber Mosaic Virus pada tomat dan mentimun (Raupach et al,1996), juga mengurangi gejala penyakit antraknos dan layu fusarium pada mentimun (Liu et al,1995). Bakterisasi dengan Serratia marcescens NBRI1213 meningkatkan aktifitas enzim Peroksidase pada daun dan akar tanaman sirih yang tahan terhadap Phytopthora nicotiana (Lavania et al, 2006).…”