Induction of permeability change and restoration of membrane permeability barrier in transformed cell cultures*1

Makan, Nizar R.

doi:10.1016/0014-4827(78)90501-3

Cited by 56 publications

(26 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In earlier work with serum-fed cells, many compounds were tested. A positive effect was observed only with ATP (1)(2)(3)(4)(5) and with y-thio-ATP (4). A considerable literature has accumulated on the effect of vanadate on a number of enzyme systems (16,17), and vanadate has been reported to be a powerful inhibitor present in ATP isolated from muscle (18,19 The cultures were preincubated with or without the various inhibitors for 5 min and then some of the cultures received 40MM ATP.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Control of membrane permeability by external and internal ATP in 3T6 cells grown in serum-free medium.

Dicker¹,

Heppel²

1980

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

ABSTLACT Cultures of 3T6 cells were plated in serum-free medium and grown in the presence of insulin (1 gg/ml) and epidermal growth factor (0.5 ng/ml). External ATP (250 ,M) applied to such cultures caused a rapid efflux of acid-soluble pools labeled with [4Hjuridine, or (1, 2). Treatment with ATP also allows the entry of normally impermeable molecules such as p-nitrophenyl phosphate, glucose 6-phosphate, or NAD (3-5). These effects are readily reversible and the treated cells grow normally afterward (1). The effect of exogenous ATP depends on the concentration of intracellular ATP; a decrease in intracellular ATP leads to a marked increase in the sensitivity of the cells to exogenous ATP (2). These findings have a number of implications. They provide a useful method by which to study internal metabolism in permeabilized cells (5, 6). Furthermore, these observations may have considerable relevance to the regulation of passive permeability, to the role of phosphorylation reactions in this control, and to the surface changes taking place after malignant transformation.The complex nature of serum and its multiplicity of actions have frequently rendered difficult the interpretation of experiments with cultured animal cells grown in medium supplemented with serum. One aspect of this problem is that proteins foreign to the cell and derived from serum present in the nutrient medium become strongly associated with the cell surface (7,8). Such adsorbed proteins may behave as membrane antigens in cell-mediated cytotoxicity (9), can be labeled by chemical probes (10) (Sigma) in phosphate-buffered saline. As soon as the cells detached from the dish, a 10-fold excess of soybean trypsin inhibitor (Sigma) was added and the cells were centrifuged 1000 X g for 10 min. The cell pellet was resuspended at 105 cells per ml in a 1:1 mixture of DME medium and Waymouth's medium supplemented with 1.6 ,M ferrous sulfate, insulin at 1 Mg/mi, and EGF at 0.5 ng/ml. This suspension was added to Nunc dishes (2 ml for 30-mm dishes, 10 ml for 90-mm dishes) and cultures were used for experiments after a confluent layer had formed (7-12 days

show abstract

Section: Resultsmentioning

confidence: 99%

“…External (1,2). Treatment with ATP also allows the entry of normally impermeable molecules such as p-nitrophenyl phosphate, glucose 6-phosphate, or NAD (3)(4)(5). These effects are readily reversible and the treated cells grow normally afterward (1).…”

mentioning

confidence: 99%

Control of membrane permeability by external and internal ATP in 3T6 cells grown in serum-free medium.

Dicker¹,

Heppel²

1980

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…First, since spontaneous "background" extracellular PPi elaboration occurs in the absence of exogenous NTP (1,2), it is possible that part or all of the PPi liberated in the presence of NTP results from augmented endogenous secretion by chondrocytes stimulated by the NTP or by an NTP metabolite. Second, extracellular ATP has been demonstrated to increase the permeability of some cell types (5,6) and cause degranulation of others (7), thus providing another route by which extracellular PPi could appear following exposure of cells to NTP.…”

Section: Arthritis and Rheumatism Vol 28 No 4 (April 1985)mentioning

confidence: 99%

Cartilage nucleoside triphosphate pyrophosphohydrolase. II. role in extracellular pyrophosphate generation and nucleotide metabolism

Ryan

Wortmann

Karas

et al. 1985

Arthritis & Rheumatism

View full text Add to dashboard Cite

Extracellular generation of inorganic pyrophosphate (PPi) in cartilage organ culture is markedly augmented by ATP. ATP, not an ATP metabolite (ADP, AMP, adenosine), is necessary for this augmentation. Excess PPi production is effectively blocked by known inhibitors of nucleoside triphosphate (NTP) pyrophosphohydrolase (EDTA, EGTA, dithiothreitol). Excess 32P-PPi is generated directly from g2P-ATP by cartilage, as substrate and product have similar specific activities. These findings strongly favor ecto-NTP pyrophosphohydrolase as the source of extracellular PPi generation in the presence of NTP. Additionally, active nucleotide and nucleoside catabolism is demonstrated in these cartilage organ cultures.Calcium pyrophosphate dihydrate (CPPD) crystal deposition disease is defined by the presence of CPPD crystals in articular fluid or tissues. These crystals often cause an inflammatory arthritis (pseudo-

show abstract

“…Recent publications have suggested that external ATP could significantly influence the permeability barrier-function of the membrane. The addition of external ATP to monolayer cultures of several transformed cells, including 3T6, SV3T3, and HeLa cells, caused a striking increase in passive permeability, allowing passage through the plasma membrane of normally impermeable molecules such as nucleotides and phosphate esters (Rozengurt and Heppel, 1975;Rozengurt, Heppel and Friedberg, 1977;Makan, 1978). Such permeability change is specific for ATP and reversible.…”

Section: Introductionmentioning

confidence: 99%

“…Since intracellular ATP concentrations were found to be similar among these cells, the different ATP-sensitivities among these classes were not ascribed to the differences in the intracellular ATP concentration. Although the mechanism of the permeability change controlled by external ATP remains unknown, involvement of protein kinase and phosphoprotein phosphatase in the plasma membrane has been suggested (Rozengurt et al, 1977;Makan, 1978). The permeability change in B16 melanoma and L929 cells was highly specific for ATP and depended on pH of the incubation medium, as was the case in transformed 3T3 cells and CHO-K1 cells (Rozengurt et al, 1977;Kitagawa and Akamatsu, 1981).…”

Section: Introductionmentioning

confidence: 99%

Different Sensitivities to External Atp in Passive Permeability of Transformed Mouse Cell Lines

Kitagawa

Akamatsu

1982

JJMSB

View full text Add to dashboard Cite

SUMMARY:External ATP causes a passive permeability change in several transformed cells but not in untransformed mouse fibroblasts.In the present experiments, the ATP-specific permeability change was observed also in B16 melanoma and L929 cells. The sensitivity to external ATP of these cells increased further when the concentration of intracellular ATP was lowered by addition of a mitochondrial inhibitor.In contrast, mouse 3T3 cells transformed by murine sarcoma virus did not respond to external ATP even when the intracellular ATP was depleted.Such differences in the ATP-sensitivity were not ascribed to the different concentrations of intracellular ATP.

show abstract

Induction of permeability change and restoration of membrane permeability barrier in transformed cell cultures*1

Cited by 56 publications

References 17 publications

Control of membrane permeability by external and internal ATP in 3T6 cells grown in serum-free medium.

Control of membrane permeability by external and internal ATP in 3T6 cells grown in serum-free medium.

Cartilage nucleoside triphosphate pyrophosphohydrolase. II. role in extracellular pyrophosphate generation and nucleotide metabolism

Different Sensitivities to External Atp in Passive Permeability of Transformed Mouse Cell Lines

Contact Info

Product

Resources

About