Induction of P-glycoprotein expression and activity by prolactin in female rat liver

Ceré, Lucila Inés; Sedlmeier, M.G.; Semeñiuk, María Belén; Luquita, Marcelo G.; Francés, Daniel E.; Ronco, Marı́a Teresa; Rigalli, Juan Pablo; Ruiz, María Laura; Catania, Viviana A.

doi:10.1016/j.lfs.2021.119936

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…After exposure to prolactin (300 µg/day, for 7 days, via osmotic minipumps), P-gp's expression and activity were assessed by Western blotting and RHO123 accumulation assay, respectively. The researchers founded that P-gp's expression and activity were increased in the presence of prolactin, suggesting a higher elimination of xenobiotics in hyperprolactinemic circumstances, including those with therapeutic purposes [126].…”

Section: In Vivo Modelsmentioning

confidence: 99%

Study Models of Drug–Drug Interactions Involving P-Glycoprotein: The Potential Benefit of P-Glycoprotein Modulation at the Kidney and Intestinal Levels

Veiga-Matos,

Morales,

Prieto

et al. 2023

Molecules

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P-glycoprotein (P-gp) is a crucial membrane transporter situated on the cell’s apical surface, being responsible for eliminating xenobiotics and endobiotics. P-gp modulators are compounds that can directly or indirectly affect this protein, leading to changes in its expression and function. These modulators can act as inhibitors, inducers, or activators, potentially causing drug–drug interactions (DDIs). This comprehensive review explores diverse models and techniques used to assess drug-induced P-gp modulation. We cover several approaches, including in silico, in vitro, ex vivo, and in vivo methods, with their respective strengths and limitations. Additionally, we explore the therapeutic implications of DDIs involving P-gp, with a special focus on the renal and intestinal elimination of P-gp substrates. This involves enhancing the removal of toxic substances from proximal tubular epithelial cells into the urine or increasing the transport of compounds from enterocytes into the intestinal lumen, thereby facilitating their excretion in the feces. A better understanding of these interactions, and of the distinct techniques applied for their study, will be of utmost importance for optimizing drug therapy, consequently minimizing drug-induced adverse and toxic effects.

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Section: In Vivo Modelsmentioning

confidence: 99%

Study Models of Drug–Drug Interactions Involving P-Glycoprotein: The Potential Benefit of P-Glycoprotein Modulation at the Kidney and Intestinal Levels

Veiga-Matos,

Morales,

Prieto

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Protein expression was detected by Western blot analyses, according to the method described previously with minor modifications (Cere et al, 2021). Use the total protein extraction kit and nuclear protein extraction kit to obtain total cellular proteins and nuclear proteins, respectively (Beyo-time).…”

Section: Western Blot Analysesmentioning

confidence: 99%

Ivermectin induces chicken BCRP/ABCG2 expression and function: Involvement of CXR signaling pathway and mRNA stabilization

Wang

et al. 2022

Vet Pharm & Therapeutics

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Ivermectin is a macrocyclic lactone antiparasitic drug widely used in human and veterinary medicine. Previous studies indicated that ivermectin could interact with Pglycoprotein, being a good inducer and substrate; however, it is unknown whether ivermectin affects BCRP of chicken. In this study, we found that ivermectin distinctly affected the expression of BCRP in a time-and concentration-dependent upregulatory way in chicken primary hepatocytes. Subsequent series of experiments showed that the BCRP induction is related with the increase of CXR expression and, promoting CXR translocations to the nucleus and enhancing the stability of Abcg2 mRNA at the post-transcriptional level by ivermectin. Furthermore, we observed that ivermectin also enhanced the stability of Abcg2 mRNA at the post-transcriptional level by Act-D chase assay. We got the similar results by in vivo test that ivermectininduced BCRP and CXR expression in pharmacologically important tissues, and decreased the apparent permeability coefficient of florfenicol (substrate of chicken BCRP). In conclusion, the results indicated that ivermectin could induce chicken BCRP expression and function through the transcriptional CXR signaling pathway and posttranscriptional mRNA stabilization.

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Effects of the SLICK1 mutation in PRLR on regulation of core body temperature and global gene expression in liver in cattle

Sosa

Santos

Rae

et al. 2022

animal

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Induction of P-glycoprotein expression and activity by prolactin in female rat liver

Cited by 3 publications

References 37 publications

Study Models of Drug–Drug Interactions Involving P-Glycoprotein: The Potential Benefit of P-Glycoprotein Modulation at the Kidney and Intestinal Levels

Study Models of Drug–Drug Interactions Involving P-Glycoprotein: The Potential Benefit of P-Glycoprotein Modulation at the Kidney and Intestinal Levels

Ivermectin induces chicken BCRP/ABCG2 expression and function: Involvement of CXR signaling pathway and mRNA stabilization

Effects of the SLICK1 mutation in PRLR on regulation of core body temperature and global gene expression in liver in cattle

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