Induction of oxidants in tomato leaves treated with DL-β-Amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis

Abstract

Bacterial canker is an economically important disease of tomato. Resistance induced by DL-b-Amino butyric acid against bacterial canker caused by Clavibacter michiganensis ssp. michiganensis in tomato plants was investigated. Different doses of DL-b-Amino butyric acid (250-1000 lg ml )1 doses) were tested on 3-week old plants inoculated with a 10 8 CFU ml )1 bacterial suspension, and disease development was evaluated after inoculation and treatment. Although in vitro growth of the bacteria was not affected by DLb-Amino butyric acid treatment, foliage sprays of 500 lg ml )1 DL-b-Amino butyric acid significantly suppressed disease development up to 54% by day 14 after inoculation at the four different doses tested. Bacterial populations were reduced by 84% in BABA-treated plants compared to water-treated plants by day 4 after inoculation. Inoculated BABA-treated plants showed significantly higher phenylalanine ammonia-lyase activity, peroxidase activity, and H 2 O 2 concentration than inoculated water-treated plants during day 1 after treatment. These findings suggest that the DL-b-Amino butyric acid treatment resulted in an increase of these enzymes and in H 2 O 2 concentration in planta, and was associated with induction of resistance to bacterial canker.

Induced systemic resistance in tomato (Solanum lycopersicum) against Botrytis cinerea by biochar amendment involves jasmonic acid signaling

“…H 2 O 2 accumulation was detected as an early response induced by bacterial speck infection in tomato pre-treated by the biocontrol agent P. putida (Ahn et al 2011). In the cases of the SAR inducing agents, DL-β-aminobutyric acid (BABA) or thiamine, activation of H 2 O 2 production takes place prior to pathogen infection (Baysal et al 2005;Boubakri et al 2012). Whether the oxidative burst observed in the current study is correlative to or needed for biochar-IR could be addressed by the use of H 2 O 2 quenching agents in future research.…”

Resistance mechanisms of wild tomato germplasm to infection of Oidium neolycopersici

“…POX catalyse oxidoreduction reactions of various substrates via H 2 O 2 ; however, they are also known to be involved in ROS production under specific conditions (Hiraga et al 2001). POX can contribute to resistance against pathogens in various pathosystems (Baysal et al 2005), whereas localized production of H 2 O 2 and superoxide radical belong among the first cytologically and histochemicaly detected responses of plant tissue on penetration of pathogen. ROS are apparently involved in the induction of hypersensitive response and they function also as signal molecules in programmed cell death (Lamb and Dixon 1997;Hückelhoven and Kogel 2003).…”

Comparative Efficacy of Systemic Acquired Resistance-Inducing Compounds Against Rust Infection in Sunflower Plants

“…It is capable of inducing systemic resistance against numerous pathogens, including Tobacco mosaic virus (TMV) (32), bacteria (2,40), oomycetes (6,33,40), ascomycetes (23,26,41), and nematodes (20,21). In Arabidopsis thaliana, BABA was shown to be effective against both necrotrophic and biotrophic pathogens, as well as abiotic stresses of drought and salinity (17,18,41).…”