Induction of Ovulation in the Diestrous Mouse by Gonadotropins

Saunders, Francis J.

doi:10.1210/endo-40-1-1

Cited by 8 publications

(6 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The present experiments confirm the previous results (Burdick et al, 1943;Saunders, 1947) even though the animals were chosen without regard to the cycle. We have also shown that mating is not a consequence of the pmsginduced ovulation.…”

Section: Discussionsupporting

confidence: 92%

“…Either they are both ovulated at one time or more than one ovulation occurs in response to the gonadotrophin treatment. Previous investigations (Burdick, Watson, Ciampa & Ciampa, 1943;Saunders, 1947) have demonstrated that, following a single injection of gonadotrophic hormone, ovulation can be induced in the postpuberal di-oestrous animals. In view of these facts, the effect of the pmsg on ovulation and mating in the post-puberal mouse during the immediate post-PMSG-injection period has been investigated.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Asynchrony of Ovulation and Mating in Mice Treated With Gonadotrophins

Stern¹,

Schuetz²

1970

Reproduction

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Asynchrony of Ovulation and Mating in Mice Treated With Gonadotrophins

Stern¹,

Schuetz²

1970

Reproduction

View full text Add to dashboard Cite

show abstract

“…The high incidence of abnormal oocytes ovulated after exogenous ovarian stimulation found in this and other studies (reviewed by McKiernan and Bavister, 1998) may be due to: (1) the production of aged or``overripened'' oocytes induced by premature ovulation of differentiated antral follicles sensitive to the LH-like activity of PMSG and later retention in the oviducts bỳ`t ube-locking'' (Edwards and Gates, 1959;Edwards and Fowler, 1960). We should note that female mice ovulate within 24 hr of a single injection of PMSG, hCG, LH, or FSH (Burdick et al, 1943;Saunders, 1947;Edwards and Fowler, 1960;Stern and Schuetz, 1970;Kaufman and Whittingham, 1972). (2) hCG-induced ovulation of degenerated oocytes that had matured when exposed to the exogenous PMSG (Edwards and Gates, 1959).…”

Section: Discussionmentioning

confidence: 65%

Stage of the estrous cycle at the time of pregnant mare's serum gonadotropin injection affects the quality of ovulated oocytes in the mouse

Tarı́n

Pérez-Albalá

Cano

2002

Molecular Reproduction Devel

View full text Add to dashboard Cite

The present study aims to analyze the effect of the stage of the estrous cycle at the time of pregnant mare's serum gonadotropin (PMSG) injection on number and quality of mouse oocytes retrieved from oviducts after exogenous ovarian stimulation. Cellular and morphological traits of ovulated oocytes from hybrid (C57Bl/6JIco female X CBA/JIco male) female mice of 12, 40-42, 50-52 or 57-62 weeks of age were analyzed. Superovulation was induced by a priming injection of PMSG at different stages of the estrous cycle followed after a 48-hr interval by human chrorionic gonadotropin. Injection of PMSG at diestrus-1 was associated with: (1) increased percentage of cumulus-free oocytes; (2) raised total percentage of oocytes without polar body; (3) increased total percentage of oocytes with intracytoplasmic mitochondrial aggregates; (4) decreased percentage of oocytes with a normal distribution of chromosomes in the metaphase II plate; and (5) raised percentage of oocytes with chromosome scattering when compared to injection at estrus, diestrus-2, and proestrus stage. On the contrary, estrus females displayed the highest percentage of oocytes with a normal distribution of chromosomes in the metaphase II plate and the lowest percentage of oocytes denuded of cumulus cells, without polar body, with intracytoplasmic mitochondrial aggregates and/or with chromosome scattering. These data suggest that administration of gonadotropins in mice should be synchronized with the innate estrous cycle of females to optimize the quality of oocytes collected from oviducts.

show abstract

“…Sexually mature female mice will ovulate within 24 hr of an injection of pmsg, hcg, lh or fsh (Burdick & Whitney, 1941;Burdick, Watson, Ciampa & Ciampa, 1943;Saunders, 1947;Edwards & Fowler, 1960;Stern & Schuetz, 1970). The number of oocytes ovulated are similar to the number released at normal oestrus (Edwards & Fowler, 1960; Kaufman, unpublished data) but unless the mice are injected immediately before oestrus, they do not mate (Stern & Schuetz, 1970).…”

mentioning

confidence: 99%