Induction of ovulation in goldfish by clomiphene citrate

Pandey, S.; Hoar, William S.

doi:10.1139/z72-222

Cited by 27 publications

(10 citation statements)

References 6 publications

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“…Here we show that exposing fish oocytes to DES at a dose within a range similar to that used in experimental exposure to 17,20␤-DHP induces oocyte maturation. Estradiol-17␤ has been reported to be ineffective in inducing fish oocyte maturation (11,12) and even inhibitory in several teleost species (13)(14)(15). Thus, the stimulatory effect of DES to induce fish oocyte maturation observed in this study has not been published previously.…”

mentioning

confidence: 49%

Diethylstilbestrol induces fish oocyte maturation

Tokumoto

Horiguchi

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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O ocyte maturation in lower vertebrates is triggered by maturation-inducing hormone (MIH), which acts on receptors located on the oocyte membrane and induces the activation of maturation-promoting factor in the oocyte cytoplasm (1-4). During the course of maturation, oocytes undergo drastic morphological changes associated with progression of the meiotic cell cycle, in which breakdown of the oocyte nuclear envelope [germinal vesicle breakdown (GVBD)] occurring at the prophase͞metaphase transition is usually regarded as a hallmark of the progress of oocyte maturation. Two MIHs, 17␣,20␤-dihydroxy-4-pregnen-3-one (17,20␤-DHP) and 17␣,20␤,21-trihydroxy-4-pregnen-3-one (20␤-S), have been identified in several fish species (5, 6). In goldfish, 17,20␤-DHP has been shown to induce oocyte maturation by stimulating the de novo synthesis of cyclin B, a regulatory subunit of maturationpromoting factor (7). Although progestins including 17,20␤-DHP and 20␤-S are the most potent steroid inducers of oocyte maturation in fish, other hormones such as deoxycorticosterone and testosterone, but not estradiol or its analogs, are also effective (8).Several endocrine-disrupting chemicals, Kepon and dichlorodiphenyldichloroethane, have been reported to antagonize MIH-induced meiotic maturation of fish oocytes in vitro (9). One of the environmental endocrine-disrupting chemicals (EEDCs), diethylstilbestrol (DES) is a nonsteroidal substance that was prescribed from the late 1940s to the early 1970s to pregnant women to prevent abortion, preeclampsia, and other complications of pregnancy. Male and female offspring exposed in utero to DES may develop multiple and neoplastic lesions of the reproductive tract, along with other changes, during development (10). Here we show that exposing fish oocytes to DES at a dose within a range similar to that used in experimental exposure to 17,20␤-DHP induces oocyte maturation. Estradiol-17␤ has been reported to be ineffective in inducing fish oocyte maturation (11, 12) and even inhibitory in several teleost species (13-15). Thus, the stimulatory effect of DES to induce fish oocyte maturation observed in this study has not been published previously. This report shows that EEDC can potentially induce oocyte maturation like an endogenous MIH, 17,20␤-DHP. Materials and MethodsMaterials. Goldfish were purchased from a local supplier and maintained at 15°C until used. Zebrafish were maintained at 28.5°C on a 14-h light͞10-h dark cycle (16). 17,20␤-DHP, DES, DES dimethyl ether (DM-DES), DES dipropionate (DP-DES), and 17␤-estradiol were purchased from Sigma. Dimethylstilbestrol (DMS) was a generous gift from J. Katzenellenbogen (University of Illinois, Urbana). 17␣-Estradiol, ethynylestradiol, butyl benzyl phthalate, di(2-ethylhexyl)-phthalate, and pentachlorophenol were obtained from Wako Pure Chemical (Osaka). Other chemicals were purchased as follows: hexestrol (HEX; ICN); trans,trans-dienestrol(␣-dienestrol) (DIES; U.S. Pharmacopeia, Rockville, MD); resveratorol (Calbiochem); DDTs (AccuStandard, New Haven, ...

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mentioning

confidence: 49%

Diethylstilbestrol induces fish oocyte maturation

Tokumoto

Horiguchi

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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“…In goldfish with mature ovaries, ovulation is induced within one or two days of a temperature increase from cold (approximately 12" C) to warm (approximately 20°C) water (Yamamoto et al, 1966); this technique has been used in several studies of ovulation in this species (Yamazaki, 1965;Yamamoto and Yamazaki, 1967;Stacey et al, 1979). The stated or implicit assumption in these and related investigations (Pandey & Hoar, 1972;Pandey et al, 1973Pandey et al, , 1977Stacey & Pandey, 1975;Lam et al, 1975Lam et al, , 1976Peter et al, 1978) is that temperature is the major factor regulating the occurrence of spontaneous ovulation in goldfish; thus, whereas, ovaries of fish kept in cold water will develop to a preovulatory condition (tertiary yolk stage, Yamazaki, 1965), ovulation will not occur until water temperature reaches some critical level. However, there is a suggestion that factors other than temperature play a role in ovulation; Yamazaki (1965) states that the presence of sexually active male goldfish, or aquatic vegetation stimulates ovulation in the goldfish, and it has been assumed that standing water is a necessary stimulus for ovulation .…”

Section: Introductionmentioning

confidence: 99%

Spontaneous and gonadotropin‐induced ovulation in the goldfish, Carassius auratus L: effects of external factors

Stagey

Cook

Peter

1979

Journal of Fish Biology

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Spontaneous ovulation in goldfish is synchronized with photoperiod and influenced by water temperature and aquatic vegetation. As the latency to ovulation from injection of HCG is highly temperature dependent, the finding that ovulation occurs at approximately the same time of day at temperatures from 12' to 26" C suggests the time of the endogenous preovulatory gonadotropin surge may change with the temperature. The time of spontaneous ovulation adjusts to a reversed 1ight:dark cycle within 2 weeks; some 4-6 h shifts in a single 1ight:dark cycle modify the time ofovulation.Few sexually mature females kept under long photoperiod (16L8D) and transferred from cold (1 3 * I" C ) to warm (2 I 1" C ) water ovulated spontaneously. Exposure to artificial aquatic vegetation for as little as one light phase significantly increases the proportion of fish ovulating in warm water. Fish kept in cold water without vegetation do not ovulate; the addition of vegetation induces ovulation, although the response latency is longer than in warm water. Aquatic vegetation may be an effective stimulus for ovulation in other teleosts which spawn on this substrate.

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“…Our justification for omitting the IP saline control i s that there is ample evidence from previous studies (Lam ei al. 1975; experiment 1 of this paper; Pandey and Hoar 1972;Pandey ef al. 1973;Stacey and Pandey 1975) that gravid goldfish injected intraperitoneally with saline will not ovulate at 12 f 1 "C.…”

Section: Methodsmentioning

confidence: 90%

“…The source and the details of maintenance of the fish (goldfish Carassius auratus, of the comet variety) were described previously (Pandey and Hoar 1972). Goldfish kept at a temperature of 12 f 1 "C under a long photoperiod (16 h light alternating with 8 h darkness) and fed with brine shrimps twice a day attain sexual maturity but do not ovulate (references cited in Introduction).…”

Section: Methodsmentioning

confidence: 99%

“…Similarly, in the goldfish Carassius auratus, relatively high doses of synthetic LH-RH injected either intraperitoneally (10 pglg) or intracranially (2 pg/g) were able to induce ovulation (Lam et al 1975). The goldfish were kept at 12 f 1 "C, a temperature at which no ovulation will occur without hormonal treatment (Yamamoto et al 1966;Yamamoto and Yamazaki 1967;Pandey and Hoar 1972;Pandey et al 1973 ;Stacey and Pandey 1975 ;Lam et al 1975).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of synthetic luteinizing hormone – releasing hormone (LH–RH) on ovulation and pituitary cytology of the goldfish Carassius auratus

Tj¹,

Pandey²,

Nagahama³

et al. 1976

Can. J. Zool.

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Synthetic luteinizing hormone – releasing hormone (LH–RH) induces ovulation and stimulates release of secretory granules from the pituitary gonadotrophs of goldfish kept at 12 ± 1 °C, a temperature at which ovulation does not occur in control fish. Gonadal development is stimulated at doses too low to induce ovulation. Relatively high doses were required to induce ovulation; this may be related to the low experimental temperatures, although several other possibilities are discussed. Intracranial injections appeared to be only slightly more effective than intraperitoneal injections. A single type of gonadotroph containing two types of granules was identified at the ultrastructural level. Only the small, electron-dense granules are released after injection of synthetic LH–RH; the role of the large, less electron-dense granules is uncertain.

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Induction of ovulation in goldfish by clomiphene citrate

Abstract: Clomiphene citrate induced ovulation in gravid goldfish. The stripped eggs were viable and on fertilization developed into normal fry.

Cited by 27 publications

References 6 publications

Diethylstilbestrol induces fish oocyte maturation

Diethylstilbestrol induces fish oocyte maturation

Spontaneous and gonadotropin‐induced ovulation in the goldfish, Carassius auratus L: effects of external factors

Effect of synthetic luteinizing hormone – releasing hormone (LH–RH) on ovulation and pituitary cytology of the goldfish Carassius auratus

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