Induction of osteogenic differentiation of osteoblast-like cells MG-63 during cultivation on fibroin microcarriers

Primary osteoblasts, osteoblast precursor cells or osteoblast‐like cell lines: Which human cell types are (most) suitable for characterizing 45S5‐bioactive glass?

“…They are often considered to be "osteoblast-like" and are therefore frequently used in tissue engineering studies that investigate the impact of BGs on osteogenic differentiation, viability and proliferation (Chan et al, 2018;Ferraz et al, 1999;Gong et al, 2012;Hoppe et al, 2015;Itala et al, 2002;Lopes et al, 2011;Lopes et al, 2016;Miola et al, 2014;Porwal et al, 2015;Price et al, 1997;Thavornyutikarn et al, 2019;Vitale-Brovarone et al, 2007;Wang et al, 2011). (Kotliarova et al, 2016). However, Clover and coworkers described that MG-63 cells indeed mimic cells isolated from human trabecular bone in terms of production of extracellular matrix proteins like osteocalcin, but do not…”

“…MG-63 cells were used for assessment of proliferation and viability in contact to 1393-BG doped with cobalt in different concentrations in a study conducted by Hoppe et al (Hoppe et al, 2015), where they example, the group of Kotliarova reported increasing ALP activity of MG-63 cells in contact to fibroin microcarriers (Kotliarova et al, 2016). However, Clover and coworkers described that MG-63 cells indeed mimic cells isolated from human trabecular bone in terms of production of extracellular matrix proteins like osteocalcin, but do not…”

Silk‐based microcarriers: current developments and future perspectives

“…The silkworm is a native insect from North China and currently the most used in silk production, being Bombyx mori, the most common species of domesticated silkworm [27]. Regarding the type and origin of silk, most of the literature reported studies to use Bombyx mori cocoons as silk source [17, 28, 29]. Silk threads from silkworm cocoons consist primarily of two protein components, fibroin, a semi‐crystalline fibrillar protein, and sericin, the water‐soluble glue‐like protein that operates as a binder to maintain the structural integrity of the cocoon [26] (Fig.…”

“… Illustration of the conventional structure of (a) Silk‐based MCs reported in the literature: (A1) fibroin/alginate MCs based on [58], (A2) fibroin/gelatin MCs based on [59], (A3) fibroin/calcium phosphate adapted based on [29] and (A4) fibroin/pullulan based on [60], (b) Silk‐based microparticles: fibroin/collagen (B1) based on [61], fibroin/chitosan (B2) based on [62]…”

“…Fibroin MCs modified by calcium phosphate mineralisation have also been studied. In the work of Kotliarova et al [29] and Goncharenko et al [81], porous MCs with the size ranging from 100 to 250 μm were obtained by cryodestruction of the matrices to generate fibroin, fibroin/gelatin, fibroin/calcium phosphate and fibroin/gelatin/calcium phosphate materials. Osteosarcoma cell lines (MG‐63) and MSCs cell adhesion occurred for all types of MCs.…”