Induction of Neutralizing Antibodies and Immunity in Vaccinated Guinea Pigs by Cyanogen Bromide-Peptides of VP3 of Foot-and-Mouth Disease Virus

Kaaden, Oskar‐Rüger; Adam, Karl-Heinz; Strohmeier, Karin

doi:10.1099/0022-1317-34-2-397

Cited by 60 publications

(19 citation statements)

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“…As had been found previously with other aphthovirus strains (Laporte et al, 1973;Bachrach et al, 1975;Kaaden et al, 1977;Meloen et al, 1979) this anti-VP1 serum was able to neutralize virus infectivity (Table 2). Since infectivity is a function of the intact particle, it follows that the VP1-A determinant must contain at least one determinant involved in the neutralization of viru~infectivity.…”

Section: The Vp1-a Determinant Contains a Determinant Involved In Virsupporting

confidence: 65%

“…Seven distinct serotypes and numerous subtypes have been defined by serological analysis (Brooksby 1958). From studies on antisera raised against the purified isolated polypeptides, the major immunizing determinant for virus neutralization has been assigned to the specific virus protein, VPI (Laporte et al, 1973;Bachrach et al, 1975;Kaaden et al, 1977;Meloen et al, 1979). Furthermore, Strohmaier et al (1982) have suggested two immunizing sites for neutralizing antibody within the carboxy-terminal third of the polypeptide based on studies using cyanogen bromide-cleaved fragments.…”

Section: Introductionmentioning

confidence: 99%

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Four Independent Antigenic Determinants on the Capsid Polypeptides of Aphthovirus

Haresnape

McCahon

1983

Journal of General Virology

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Section: The Vp1-a Determinant Contains a Determinant Involved In Virsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Four Independent Antigenic Determinants on the Capsid Polypeptides of Aphthovirus

Haresnape

McCahon

1983

Journal of General Virology

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“…With several strains of virus this cleavage results in a considerable reduction of the infectivity titre and immunizing activity (Wild & Brown, 1967;Wild et al 1969;Rowlands et al 197I). This result provided good evidence that only VPI was involved in the immunizing activity of the I46S particles and the demonstration by Laporte et al (I973), Bachrach et al (1975), Kaaden et al (1977 and Meloen et al (1979) that isolated VP1 will stimulate the production of neutralizing antibody and protect animals against challenge has provided more direct evidence for its role in immunization.…”

Section: Discussionmentioning

confidence: 61%

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus

Cartwright

Chapman

Brown

1980

Journal of General Virology

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SUMMARYIntact 146S particles of the seven serotypes of foot-and-mouth disease virus (FMDV) produce type-specific precipitating, complement-fixing and neutralizing antibodies in cattle and guinea-pigs. However, the 12S structural subunit, produced from the virus particle by mild acid treatment (pH 6) or by heating at 56 °C, although stimulating the production of precipitating and complement-fixing antibodies, produces only low levels of neutralizing antibody. Nevertheless, IzS particles were as active as 146S particles in stimulating the production of neutralizing antibody in guinea-pigs primed with a vaccine prepared from L46S particles. Moreover, heterotypic ~zS and I46S particles also boosted the neutralizing antibody response to the first virus. These results point to an antigenic similarity between the I46S particles of each type and to a close antigenic relationship between the r46S and IzS particles.

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“…Antibodies against polypeptide VP1 of foot-and-mouth disease virus (FMDV) prepared in a similar way did have neutralizing activity (Meloen et al, 1979). The observation that antisera against purified preparations of VP1 of FMDV types O and A had neutralizing activity was also reported earlier (Laporte et al, 1973;Bachrach et al, 1975;Kaaden et al, 1977).…”

Section: Introductionsupporting

confidence: 50%

Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

Wiegers

Dernick

1983

Journal of General Virology

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SUMMARYPure poliovirus polypeptides, namely VP1, VP2, VP3 and VP4, have been isolated by isoelectric focusing after dissociation of poliovirus by urea. When injected into rabbits, all four polypeptides produced monospecific antisera which were used for the characterization of poliovirus particles and poliovirus-infected cells. The specificity of these antisera was determined by immunoprecipitation of polypeptides obtained by dissociation of poliovirus with SDS, followed by characterization by polyacrylamide gel electrophoresis. The antisera revealed differences in the antigenic sites of native poliovirus particles, heated poliovirus particles and naturally occurring empty capsids. Only VP3 antiserum reacted with native poliovirus and showed some neutralization, whereas all antisera precipitated heated virus and empty capsids. These antisera reacted also with the appropriate precursors of the capsid polypeptides demonstrating their usefulness for an analysis of the cleavage pathway by monospecific antibodies and revealed a second protomer (90 kilodalton) polypeptide for the capsid proteins of poliovirus particles.

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Induction of Neutralizing Antibodies and Immunity in Vaccinated Guinea Pigs by Cyanogen Bromide-Peptides of VP3 of Foot-and-Mouth Disease Virus

Cited by 60 publications

References 0 publications

Four Independent Antigenic Determinants on the Capsid Polypeptides of Aphthovirus

Four Independent Antigenic Determinants on the Capsid Polypeptides of Aphthovirus

Serological and Immunological Relationships between the 146S and 12S Particles of Foot-and-Mouth Disease Virus

Monospecific Antisera Against Capsid Polypeptides of Poliovirus Type 1 Distinguish Antigenic Structures of Poliovirus Proteins

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