Induction of neural-like cells and acetylcholinesterase activity in cultures of F9 teratocarcinoma treated with retinoic acid and dibutyryl cyclic adenosine monophosphate

Increase in cell‐surface N‐acetylglucosaminide β(1→4)galactosyltransferase activity with retinoic acid‐induced differentiation of F9 embryonal carcinoma cells

“…Cyclic AMP has been shown to promote retinoic acid-induced differentiation of F9 cells to endoderm [6,15,16]. In agreement with these studies, in F9 cells treated for 3 and 6 days with retinoic acid and for an additional 24 h with retinoic acid in the presence and absence of 8-bromo cyclic AMP, cyclic AMP elicits a further 2-fold increase 2).…”

Sodium butyrate induces histone hyperacetylation and differentiation of murine embryonal carcinoma cells.

“…In that study and others, dibutyryl cAMP, when added along with RA, was shown to enhance the phenotypic differences between EC cells and the ultimate differentiated derivatives (23,(36)(37)(38)(39) . However, the enhancing effect was presumably due to the cAMP rather than the butyrate moiety since 606 THE JOURNAL OF CELL BIOLOGY " VOLUME 98,1984 agents such as cholera toxin elicit the same response (23).…”

Retinoic Acid Induces Expression of the Thyroid Hormone Transporter, Monocarboxylate Transporter 8 (Mct8)

“…Cells were subcultivated at a ratio of 1:10 to 1:20 and used at passages 2-6, unless otherwise noted. Neuron-like cells were induced as described previously (15) with minor modifications. Briefly, ϳ5 ϫ 10 5 cells seeded in gelatin-coated Petri dishes were maintained in DMEM/F-12 medium (Invitrogen) with 2% FBS, 1 M tRA, and 1 mM 8-bromo-cAMP for up to 7 days.…”

“…Briefly, total RNA from culture cells was prepared with the RNeasy minikit (Qiagen, Valencia, CA) with on-column DNase digestion. Three g of total RNA was reverse-transcribed by using 50 units of Superscript III reverse transcriptase (Invitrogen) in a 20-l reaction with oligo-(dT) [12][13][14][15][16][17][18] primer or random hexamer. Quantitative PCR of mouse thyroid hormone transporter genes, glyceraldehyde-3-phosphate dehydrogenase gene (Gapdh), 18 S ribosomal RNA, and human MCT8 mRNA was performed with custom DNA primers synthesized by Invitrogen (supplemental Table 1).…”

“…A combination treatment of F9 cells with all-trans-RA (tRA), an agonist of RAR, and cAMP induces extraembryonic endoderm (13,14) or neuron-like cells (15), depending on the composition of culture media. The extraembryonic endoderm supports the developing embryo and facilitates exchange of small molecules between the maternal circulation and the embryo, functioning as an "early placenta" (16).…”