Induction of mutations in Browallia speciosa using sodium azide and identification of the genetic variation by peroxidase isozyme

Abstract

Plants of Browallia speciosa were treated with different concentrations of sodium azide (0, 200, 400, 600 and 800 ppm) as a soil drench. The concentration of 800 ppm had simulative effect on the most studied traits. It increased the number of branches and leaves, chlorophyll content, fresh weights of vegetative growth and roots, dry weights of vegetative growth and roots and root length in the M 1 (2011/ 2012) and M 2 (2012/ 2013). All the concentrations of sodium azide produced changes in the flower colour, flower shape and leaf form in both generations. Peroxidase isozyme pattern indicated that seven loci produced of peroxidase are shown in B. speciosa. In addition, different peroxidase profiles among mutants were found. The phylogenetic tree indicated that mutant 4 was the most genetically distinct mutant from the control followed by mutant 3.

Morphological and Phenotypic Effects of Fast Neutron Irradiation (FNI) on Lagos Spinach (<i>Celosia argentea</i> L.)

“…Insignificant increased in stem diameters recorded in irradiated plants when compared with the control in this study, agrees with the work of El-Mokadem and Mostafa [22]. They reported insignificant effects of different sodium azide concentration with respect to stem diameter in the M 1 generation of Browallia speciosa.…”

Mutation Induction of Dendrobium ‘Earsakul’ Using Sodium Azide

“…When bluebells were treated with different NaN 3 concentrations, the highest (12.3 mM) NaN 3 concentration was found to increase the number of branches and leaves, chlorophyll content, fresh weights of shoots and roots, dry weights of shoots and roots, and root length. In addition, all concentrations (3.7, 6.15, 9.2, and 12.3 mM) of NaN 3 induced changes in flower color, flower shape, and leaf form (El-Mokadem and Mostafa, 2014). Identification of mutants with ISSR markers.…”

“…In sunflowers (Helianthus annuus L.), mutagenic NaN 3 produced mutants whose seeds contain 35% stearic acid ( Skori c et al, 2008). In bluebell (Browallia speciosa), NaN 3 induced changes in flower color, flower shape, and leaf form (El-Mokadem and Mostafa, 2014). Whereas, in the terrestrial orchid (Spathoglottis plicata), NaN 3 induced strikingly attractive flower color modifications (Roy and Biswas, 2005).…”

X-ray Induced Genetic Variability in <i>Amaranthus hybridus</i> L. and Analysis of Variants Using Morphological and Random Amplified Polymorphic DNA Data

“…Mutations create variations in the gene pool, and the less favorable (or deleterious) mutations are removed from the gene pool by natural selection, while more favorable (beneficial or advantageous) ones tend to accumulate, resulting in evolutionary change [6]. Irradiation has been successfully used for mutation breeding in various crops and ornamental plants [37] and has proven an adept means of encouraging the expression of recessive genes and producing new genetic variations [23,[36][37][38]. A study [22] reported that the height of Zea mays coleoptiles exposed to X-ray doses up to 500 r was not affected and shoots of the plants which received over 800 r showed the effects of irradiation by delay in bursting through the coleoptiles and failure to reach the height attained by their controls during the time of the experiment.…”