Induction of murine gamma interferon production by lipopolysaccharide and interleukin-2 in Propionibacterium acnes-induced peritoneal exudate cells

Abstract

Lipopolysaccharide (LPS) induces high levels of gamma interferon (IFN-,y) in the circulation of mice pretreated with heat-killed Propionibacterium acnes. The following results were obtained in the present study. (i) LPS, as well as interleukin-2 (IL-2), was also able to induce IFN-y in vitro in peritoneal exudate cells (PEC) from such mice. Splenocytes and lymph node cells from these mice or resident peritoneal cells from control mice produced trace or undetectable amount of IFN-y upon exposure to LPS. (ii) A synergistic effect on IFN-'y induction was observed when LPS was added to a culture of PEC together with IL-2. (iii) Indomethacin augmented the induction of IFN-y by LPS or IL-2, and prostaglandin E2 reversed its effect. (iv) Deprivation of plastic-adherent or nylon wool-adherent cells abolished the induction by LPS or IL-2, whereas it did not affect that by concanavalin A. (v) Culture supernatant of plastic-adherent cells incubated with LPS stimulated the nylon wool-nonadherent cells to produce IFN-y in the presence of IL-2, but interleukin-1 or phorbol myristic acetate did not replace the LPS-stimulated supernatant. (vi) The ability of PEC to produce IFN-y measured as a function of time after P. acnes injection increased in proportion to their natural killer (NK)-like activity against YAC-1 cells. Moreover, treatment of PEC with monoclonal anti-Thy-1 antibody or with anti-asialo GM1 antiserum plus complement eliminated the production of IFN-y and the NK-like activity simultaneously, whereas treatment with monoclonal anti-Lyt-2 antibody plus complement did not. These results suggest that IL-2 and some unidentified factor released from plastic-adherent cells by LPS stimulation cooperatively induce IFN-y production in activated, Thy-iand asialo GM1-positive NK-like cells appearing in inflammatory reactions and that prostaglandin E2 regulates IFN-y production in these cells.

In Vitro and In Vivo Effects of an Immunomodulator Composed of Escherichia Coli Lipopolysaccharide and Propionibacterium granulosum‐Inactivated Cells in Pigs

“…Regarding IFN-g our results showing high levels of production in INMD-stimulated cells indicated that combination of Propionibacterium and LPS in pigs produced a higher induction potential than any compounds alone over NK or other IFN-g-producing cells in pigs. This has been observed before by other authors in humans and mice (Hirt et al, 1978;Okamura et al, 1982;Kirchner et al, 1986;Okamura et al, 1987).…”

Interferon-γ, interleukin-1 and tumour necrosis factor-α synthesis during experimental murine staphylococcal infection

“…Lipopolysaccharides (LPS) of Gram-negative bacteria are potent stimulants of many cytokines, such as IL-1, IL-6 and INF-a [1,5,10,11]. Until recently, few studies of cytokine stimulating activity of different Gram-positive bacteria and their cell surface components and extracellular products have been available [1,8,11,12,16,17]. However, it is known that some of the symptoms in septic shock, caused by S. aureus, may be associated with overproduction of IL-1 and TNF-a stimulated by toxic shock syndrome toxin-1 (TSST-1) [1,5,12].…”

“…It should be noted, however, that because of the complex, regulatory functions of IFN-7, contribution of this lymphokine to the pathogenesis of other infections is also important. IFN-y enhances different functions of macrophages and stimulates the antibody secretion of B cells [1,5,16,22,29]. IFN-T also increases in vitro production of TNF-a by cultured macrophages and probably augments synthesis and their biological activity in vivo.…”

Interleukin-18/interferon-γ-inducing factor, a novel cytokine, up-regulates ICAM-1 (CD54) expression in KG-1 cells

“…It has recently been reported that mice injected with heat-killed Propionibacterium acnes released significantly higher levels of interferon-␥ (IFN-␥) into the circulation on challenge with T cell mitogens than mice challenged with T cell mitogens alone [1][2][3][4]. This IFN-␥ production was also induced in vivo when using lipopolysaccharide (LPS) as a mitogen instead of T cell mitogens [5][6][7].…”