Induction of multiple replacement mutations by oligonucleotide-directed mutagenesis with extended mismatch primers

Lichtler, Alex; Hager, Gordon L.

doi:10.1016/0735-0651(87)90009-4

Gene Analysis Techniques

1987

DOI: 10.1016/0735-0651(87)90009-4

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Induction of multiple replacement mutations by oligonucleotide-directed mutagenesis with extended mismatch primers

Alex Lichtler

Gordon L. Hager

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1988

1993

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Cited by 5 publications

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“…Digestion products were separated on an 8% denaturing urea gel, which was subjected to autoradiography. Primer-extension analysis was performed as described (10 (8,21). After introduction of the chicken PR by transient transfection, we compared the activity of the receptor on the genomic MMTV promoters and transiently cotransfected templates.…”

mentioning

confidence: 99%

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

Smith

Archer

Hamlin-Green

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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During development and differentiation, the expression of banscription factors is regulated in a temporal fashion. Newly expressed transcription factors must interact productively with target genes organized in chromatin. Although the mechanisms governing factor binding to chromatin templates are not well understood, it is now clear that template access can be dramatically influenced by nudeoprotein structure. We have examined the ability of a well characterized ranwactivator, the progesterone receptor (PR), to activate the mouse mammna tumor virus (MMTV) promoter organized either in stable, replicating templates that have a highly ordered nucleosome structure or as transiently transfected DNA, which adopts a less-dermed structure. (8). In this structural configuration, nuclear factor 1 (NF1) is excluded from its target site in the proximal promoter (1, 6, 9, 10). Binding of the activated glucocorticoid receptor (GR) to the promoter induces a chromatin remodeling event associated with the second nucleosome (Nuc-B) (8, 11). In addition, histone Hi is depleted from the promoter proximal region in a hormone-dependent manner (12). We proposed previously that this chromatin transition is directly and mechanistically involved in the binding of NF1 and subsequent formation of the transcription preinitiation complex (9, 10). Similar phenomena have been observed for the murine tyrosine aminotransferase (13-15) and yeast phoS (2,16) genes.The progesterone receptor (PR) and GR activate the MMTV promoter through the same target sequences as determined by transient transfections (17)(18)(19)(20)

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mentioning

confidence: 99%

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

Smith

Archer

Hamlin-Green

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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Oligonucleotide-directed mutagenesis: A sequence-based screening

Duilio

Cimino

Russo

1988

Analytical Biochemistry

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Two motion systems with common and separate pathways for color and luminance.

Goréa

Papathomas

Kovács

1993

Proc. Natl. Acad. Sci. U.S.A.

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We present psychophysical experiments that reveal two motion systems, a specific and an unspecific one. The specfflc system prevails at medium to high temporal frequencies. It comprises at least two separate motion pathways that are selective for color and for luminance and that do not interact until after the motion signal is extracted separately in each. By contrast, the unspecific system prevails at low temporal frequencies and it combines color and luminance signals at an earlier stage, before motion extraction. The successful implementation of an efficient and accurate technique for assessing equiluminance corroborates further the main findings. These results offer a general framework for understanding the nature of interactions between color and luminance signals in motion perception and suggest that previously proposed dichotomies in motion processing may be encompassed by the specific/unspecific dichotomy proposed here.

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Induction of multiple replacement mutations by oligonucleotide-directed mutagenesis with extended mismatch primers

Cited by 5 publications

References 18 publications

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.

Oligonucleotide-directed mutagenesis: A sequence-based screening

Two motion systems with common and separate pathways for color and luminance.

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