Induction of multiple ovulations in mares using low doses of GnRH agonist Deslorelin Acetate at 48 hours after luteolysis

Azevedo, Marlon Vasconcelos; Souza, Natália Matos; Ferreira-Silva, José Carlos; Batista, I. O.; Moura, Marcelo Tigre; Oliveira, M. A. L.; Alvarenga, Marco Antônio; Lima, Paulo Ricardo Silva

doi:10.21836/pem20150208

Cited by 7 publications

(13 citation statements)

References 10 publications

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“…The farm is located in the following geographic coordinates, 7 o 52' 29'' S of latitude and 35 o 27' 01'' W of longitude. The mean annual temperature is 24 o C, and quantum of rain reaches 1,248 mm 3 .…”

Section: Experimental Locationmentioning

confidence: 99%

“…The duration of the estrogenic phase in horses does not allow to predict the exact moment of ovulation [14], and follicle growth monitoring is required for ovulation assessment [3,15]. Therefore, ovulationinducing agents are investigated aiming to increase both artificial insemination (AI) and embryo transfer efficiencies [3,27,29].…”

Section: Introductionmentioning

confidence: 99%

“…The hCG was the first ovulation-inducing agent and remains as the most widely used in mares [30]. Its structure resembles the LH protein, a fact which has suggested that its ovulation-inducing properties result from a direct effect on diminishing estradiol concentration, increasing LH, and ovulation within 48-h after treatment [3,16,40]. It is advisable that when the follicle reaches 35 mm, hCG should be used for induction of ovulation [7].…”

Section: Introductionmentioning

confidence: 99%

“…The GnRH is a natural ovulation-inducing factor, although it has a short half-life [34]. The synthetic analogs of GnRH such as Buserelin [6,17], Deslorelin [1,3,4], and Histrelin [26,30,39], have greater potency and longer half-life confers greater structural and metabolic stability than its natural form [24]. Collectively, all these compounds have been considered as capable of inducing ovulation in mares.…”

Section: Introductionmentioning

confidence: 99%

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Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Ferreira-Silva¹,

Nascimento²,

Moura³

et al. 2018

Acta Scientiae. Vet.

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Background: Induction of ovulation is a key procedure for horse assisted reproduction technologies, such as for artificial insemination (AI) and embryo transfer. The application of hCG remains as the primary ovulation-inducing agent for horse assisted reproduction, but alternatives are in demand to avoid its adverse effects, such as loss of ovulation-inducing efficiency over multiple applications by hCC-antibody production. Despite reports on alternative ovulation-inducing agents, pair-wise comparisons of such agents under similar conditions have been limited. Under such scenario, the work was aimed to determine the efficiency of both hCG and Buserelin at inducing ovulation in Mangalarga Marchador mares raised in the Northeast of Brazil under an AI program.Materials, Methods & Results: Mares were initially selected based on their reproductive performance, the absence of clinical-reproductive alterations and adequate body condition score. Mares in diestrus were randomly distributed in three experimental conditions, received 5 mg of Dinoprost and were monitored daily for estrus detection. After estrus detection, ovaries were monitored by ultrasonography, in 12-h intervals, until the follicle reached 35 mm. At this time-point, ovulation was induced with 0.042 mg of Buserelin endovenously, with 3,000 IU hCG by an intramuscular shot, and control mares received 2 mL of saline solution, also by an intramuscular shot. Both hCG and Buserelin displayed similar efficiencies (P > 0.05) for induction of ovulation and that both agents were effective (P < 0.05) for such purpose, since greater percentages (P < 0.05) of induction on mares treated from those of the control. Moreover, the total number of ovulations in mares treated at the end of the experiment was not different (P > 0.05) from those found in the Control. All ovulations occurred within a 72-h period after treatment. It can be observed that in mares treated with hCG or Buserelin, ovulations occurred both in more mares (P < 0.05) and at earlier time-points than mares from the control. It is also possible to note that pregnancy was not different (P > 0.05) between hCG and Buserelin groups, and that pregnancy of mares treated with ovulation-inducing factors was similar to the control.Discussion: The majority of ovulations in mares occurred within initial 48-h after treatment for both hCG and GnRH, suggesting a similar potential for horse assisted reproduction. Both hCG and Buserelin are two commonly used agents for induction of ovulation in mares. As described here, the majority of ovulations occurred within initial 48-h after treatment, a fact which can be attributed to hCG and GnRH activity, since it can happen in intervals from 36 to 48-h after treatment. Pregnancy rates did not differ among groups. These results are under the working hypothesis that hCG and Buserelin would display similar efficiencies on pregnancy rates. Despite the understanding of hCG activity on induction of ovulation due to its high specificity toward LH receptors and results from a direct effect on diminishing estradiol concentration, increasing LH, and further inducing ovulation within 48-h after treatment. In contrast, Buserelin has a similar functional property but acts upon LH synthesis and its release. In conclusion, ovulation in mares can be induced with both hCG and Buserelin, and both ovulation-inducing agents do not affect pregnancy rates.Keywords: gonadotropin, releasing factor, ovary, follicle, corpus luteum.

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Section: Experimental Locationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Ferreira-Silva¹,

Nascimento²,

Moura³

et al. 2018

Acta Scientiae. Vet.

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“…However, the number of embryos recovered for each ovulation in mares induced to multiple ovulations has still been low and close Use of Equine Pituitary Extract (EPE) in low doses to induce double ovulation in mares to that one presented by mares with natural ovulation, around 0.5 embryos. Several products have been employed in order to increase follicles number for mares ovulation as Porcine Follicle Stimulating Hormone (p-FSH) (Cullingford et al, 2010), Equine Chorionic Gonadotrofin (ECG) and Gonadotropin Releasing Hormone (GnRH) (Farquhar et al, 2000), Equine Pituitary Extract (EPE) (Alvarenga et al, 2001(Alvarenga et al, , 2008, purified Extract of Equine Follicle Stimulating Hormone (e-FSH), (Köllmann et al, 2008) and more recently, Deslorelin Acetate (Azevedo et al, 2015). Due to the fact that results have widely varied, research is directed to protocols using EPE and FSH, re-FSH and re-LH combination and Deslorelin Acetate (Bertozzo et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Use of equine pituitary extract (EPE) in low doses to induce double ovulation in mares

Sá¹,

Santos²,

Bezerra³

et al. 2017

BJVM

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Association of Deslorelin and Sulpiride for Double Ovulation Induction in Mares

Bertoldi

Segabinazzi

Dell’Aqua

et al. 2018

Journal of Equine Veterinary Science

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The aim of this study was to verify the effect of deslorelin and sulpiride association on double ovulation induction in mares. Ten mares were used for this study. Eight days after ovulation, luteolysis was induced and mares were submitted a daily ultrasonographic evaluation until observation of at least two follicles measuring between 20 and 25 mm. Animals were split in two experimental groups in a crossover study. In deslorelin (DES) group, the animals received 150 mg deslorelin, intramuscularly, every 12 hours, until the second largest follicle reached at least 33 mm in diameter, at which time ovulation induction was performed with 1.500 IU of human chorionic gonadotrophin, IV. In the DES + sulpiride (SULP) group, the treatment was performed in the same manner as in the DES group, associated with the administration of 1 g of sulpiride, orally, once daily until the time of ovulations induction. In both groups, ultrasound examinations were performed daily to evaluate follicular development and the detection of ovulations. Double ovulations were observed in 70% (7/10) of the animals of the DES group and 50% (5/10) of the animals in the DES + SULP group. The percentage of ovulations corresponding to the number of follicles developed was 81.8% (18/22) for the DES group and 79% (15/19) for the DES + SULP group, and no difference was observed between the groups (P > .05). We conclude that association of sulpiride with deslorelin acetate did not contribute to the increase in double ovulations in mares.

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Induction of multiple ovulations in mares using low doses of GnRH agonist Deslorelin Acetate at 48 hours after luteolysis

Cited by 7 publications

References 10 publications

Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Induction of Ovulation in Mangalarga Marchador Mares by hCG or GnRH

Use of equine pituitary extract (EPE) in low doses to induce double ovulation in mares

Association of Deslorelin and Sulpiride for Double Ovulation Induction in Mares

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