Induction of mitotic crossing-over in diploid strains of Aspergillus nidulans using low-dose X-rays

Stoll, L. B.; Crémonesi, F.; Pires, L T A; Zucchi, Tiago Domingues; Zucchi, Tania Maria Araujo Domingues

doi:10.4238/vol7-2gmr414

Cited by 5 publications

(8 citation statements)

References 29 publications

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“…However, even when untreated, Dp II-I//UT184 displayed a typical behavior with a high level of mitotic recombination and high HI level. These and other previously reported findings (Pires and Zucchi, 1994;Zucchi et al, 2005;Stoll et al, 2008) suggest that eventual unrepaired DNA lesions were recombinogenic as well as DNA breaks resulting from the DNA replication mechanism (Pires and Zucchi, 1998).…”

Section: Homozygotization Indexsupporting

confidence: 77%

“…Based on its specific characteristics, Hg-exposed Dp II-I//UT448 was expected to show HI values significantly higher than those observed for the untreated diploid, as previous-ly reported for benzene fumes, UV and X-ray irradiation (Pires and Zucchi, 1994;Zucchi et al, 2005;Stoll et al, 2008), but contrarily, the results pointed towards a refractory behavior for this diploid, regarding Hg fumes. Specifically, this strain showed expected high values when exposed to Hg fumes (Figure 2).…”

Section: Homozygotization Indexsupporting

confidence: 67%

“…Indeed, this test can identify the environmental risks induced by single chemical agents (Zucchi et al, 2005;Miyamoto et al, 2007;Salvador et al, 2008;Stoll et al, 2008) as well as heterogeneous mixtures. In general, the use of biological systems focusing on genotoxicity detection of environmental contamination provides insight into the potential for increased mutation rates and alterations in the gene pool of various organisms (Lewis et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The HI test herein adapted to evaluate the genotoxic potential of Hg fume to induce mitotic crossing-over in diploid cells of eukaryotic microorganisms had already been extensively used and frequently mentioned in the scientific literature (Zucchi et al, 2005;Salvador et al, 2008;Stoll et al, 2008).…”

Section: Homozygotization Index Testmentioning

confidence: 99%

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Aspergillus nidulans as a biological system to detect the genotoxic effects of mercury fumes on eukaryotes

Sousa¹,

Zucchi²,

Zucchi³

et al. 2009

Genet. Mol. Res.

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ABSTRACT. Mercury (Hg) pollution is one of the most serious environmental problems. Due to public concern prompted by the symptoms displayed by people who consumed contaminated fish in Minamata, Japan in 1956, Hg pollution has since been kept under constant surveillance. However, despite considerable accumulation of knowledge on the noxious effects of ingested or inhaled Hg, especially for humans, there is virtually nothing known about the genotoxic effects of Hg. Because increased mitotic crossing over is assumed to be the first step leading to carcinogenesis, we used a sensitive short-term test (homozygotization index) to look for DNA alterations induced by Hg fumes. In one Aspergillus nidulans diploid strain (UT448//UT184), the effects of the Hg fumes appeared scattered all Quick assay to detect the genotoxicity of mercury fumes over the DNA, causing 3.05 times more recombination frequencies than the mean for other strains. Another diploid (Dp II-I//UT184) was little affected by Hg. This led us to hypothesize that a genetic factor present in the UT184 master strain genome, close to the nicB8 genetic marker, is responsible for this behavior. These findings corroborate our previous findings that the homozygotization index can be used as a bioassay for rapid and efficient assessment of ecotoxicological hazards.

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Section: Homozygotization Indexsupporting

confidence: 77%

Section: Homozygotization Indexsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Homozygotization Index Testmentioning

confidence: 99%

See 2 more Smart Citations

Aspergillus nidulans as a biological system to detect the genotoxic effects of mercury fumes on eukaryotes

Sousa¹,

Zucchi²,

Zucchi³

et al. 2009

Genet. Mol. Res.

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“…Despite such trends present shortterm test has been used to disclose suspected genotoxicity rapidly and efficiently. Besides, the test is very sensitive as genotoxicity can be detected even after application of low doses of a given agent, such as UV [13], benzene fumes [14], sodium nitroprusside [40], benznidazole [41], cisplatin and cytosine arabinoside [42] and X-ray [43]. These characters altogether allied to test inexpensiveness, suggest involved eukaryote microorganism (A. nidulans) can be proposed as a cell model to guide early approaches towards rapid screening of genotoxic potential and biological activities of many chemicals.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic Potentials of Natural Products Detected by a Short-Term Test Using Diploid Strains of Aspergillus nidulans

Salvador¹,

Zucchi²,

Schinor³

et al. 2008

TOMYCJ

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Main aim is to evaluate the genotoxic potentials of three classes of natural products, a sesquiterpene lactone (cynaropicrin), a saponin (stigmast-7en-3 -ol-glucopyranoside) and a steroid (stigmasterol), at low doses, using the Homozygotization Index test (HI). This short-term test, deals with the determination of alterations in mitotic crossing-over frequencies eventually involving heterozygous auxotrophic genes in diploid strains of Aspergillus nidulans. In this way, treatments of UT448//UT184 and Dp II-I//UT184 diploid strains with the natural products showed that saponin and lactone significantly increased the homozygotization index (HI) of both strains, especially for the diploid Dp II-I//UT184. No effect was observed with the steroid. On this account it presents remarkable potentials to detect the genotoxic/carcinogenic effects of bioactive natural products. Furthermore, this is the first time the low doses effects of bioactive natural products were detected using A. nidulans.

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Metformin's performance in in vitro and in vivo genetic toxicology studies

Sant’Anna

Yajima

Rosada

et al. 2013

Exp Biol Med (Maywood)

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Metformin is a hypoglycemiant drug prescribed for the treatment and control of the type 2 diabetes mellitus. Recently, the potential efficacy of this antidiabetic drug as an anticancer agent has been demonstrated in various mammalian cancer cells. This report evaluates the mutagenic as well as the recombinogenic potentials of the metformin drug in therapeutically relevant plasma concentrations (12.5 µM, 25.0 µM or 50.0 µM). Since the loss of heterozygosity is a process associated with carcinogenesis, the recombinogenic potential of such a drug was evaluated by the homozygotization assay using a heterozygous diploid strain of Aspergillus nidulans. The homozigotization indices (HI) for the genetic markers from the metformin-treated diploids were not statistically different from the negative control (non-treated diploids). For the first time, this indicated a lack of recombinogenic activity of the antidiabetic drug. The mutagenic potential of the metformin drug was evaluated by the chromosome aberrations and the micronuclei tests in human lymphocytes cultures. The metformin drug did not show any significant increase either in the numerical or in the structural chromosome aberrations and did not affect significantly the mitotic index when compared to the negative control. In the in vitro micronucleus test, the drug did not increase the number of micronuclei or nuclear buds when compared with the negative control. The data in this study suggest that the metformin drug is not a secondary cancer inducer, since it has neither showed recombinogenic nor mutagenic activities when used in pharmacological concentrations.

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Induction of mitotic crossing-over in diploid strains of Aspergillus nidulans using low-dose X-rays

Cited by 5 publications

References 29 publications

Aspergillus nidulans as a biological system to detect the genotoxic effects of mercury fumes on eukaryotes

Aspergillus nidulans as a biological system to detect the genotoxic effects of mercury fumes on eukaryotes

Genotoxic Potentials of Natural Products Detected by a Short-Term Test Using Diploid Strains of Aspergillus nidulans

Metformin's performance in in vitro and in vivo genetic toxicology studies

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