Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro- lide

Ren, Yan; Xiong, Lei; Wu, Jia

doi:10.1038/sj.cr.7290174

Cited by 9 publications

(8 citation statements)

References 23 publications

(23 reference statements)

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“…Tripchlorolide (TC), a derivate of triptolide with similar activity and much lower toxicity [25], has shown its anti-neoplastic activities against leukemic, lung and other tumor cells. Our previous works reported that TC disturbed the cell cycle and induced apoptosis in Chinese hamster ovary (CHO) cells, which resulted from degradation of c-Myc and dysfunctional p53 [26,27]. In this study, we demonstrated that TC inhibited the expression of viral oncoprotein E6/E7, which resulted in an up-regulation of p53 in HeLa S3 cells.…”

Section: Introductionmentioning

confidence: 58%

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

et al. 2005

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Recent studies indicate that cell-cycle checkpoints are tightly correlated with the regulation of apoptosis, in which p53 plays an important role. Our present works show that the expression of E6/E7 oncogenes of human papillomavirus in HeLa cells is inhibited in the presence of anti-tumor reagent tripchlorolide (TC), which results in the up-regulation of p53 in HeLa cells. Interestingly, under the same TC-treatment, the cells at the early S-phase are more susceptible to apoptosis than those at the middle S-phase although p53 protein is stabilized to the same level in both situations. Significant difference is exhibited between the two specified expression profiles. Further analysis demonstrates that anti-apoptotic gene survivin is up-regulated by p53 in the TC-treated middle-S cells, whereas it is down-regulated by p53 in the TC-treated early-S cells. Taken together, the present study indicates that the differential p53-regulated expression of survivin at different stages of the cell cycle results in different cellular outputs under the same apoptosis-inducer.

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Section: Introductionmentioning

confidence: 58%

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

et al. 2005

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“…14 MAPK, mitogen-activated protein kinase; TF, transcription factor; TNF-a, tumour necrosis factor-a; IL-1b, interleukin-1b. activating the mitochondrion-mediated apoptotic pathway involving Bcl-2 family proteins and cytochrome c. 77 Tripchlorolide was also shown to promote the degradation of c-Myc protein, an important oncogenic driver in many cancers. 78 …”

Section: Anti-cancer Properties Of Tw Bioactive Compoundsmentioning

confidence: 99%

Tripterygium wilfordii bioactive compounds as anticancer and anti‐inflammatory agents

Wong¹,

Yuan²,

Luk³

2012

Clin Exp Pharma Physio

130

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1. Bioactive compounds from medicinal plants with anticancer and anti-inflammatory effects have become key resources in drug discovery fields for the treatment of various malignancies and immunological disorders. 2. Tripterygium wilfordii (TW) is a medicinal plant that exhibits profound immunosuppressive effects and has been used in herbal regimens for the treatment of immunological diseases for thousand years in China. 3. Procedures for the isolation and characterization of TW bioactive compounds have been well established. Over the past three decades, more than 46 diterpenoids, 20 new triterpenoids, 26 alkaloids and other small molecules have been identified from TW. 4. Triptolide, celastrol and tripchlorolide are among the bioactive compounds conferring the immunosuppressive and anticancer activities of TW. Accumulated evidence suggests that the TW bioactive compounds exert their pharmacological actions by modulating the transcriptional activity of nuclear factor-κB signalling molecule. 5. Triptolide derivatives with improved water solubility have emerged as promising drug candidates. Clinical trials are being conducted on the derivatives to provide encyclopaedic knowledge on triptolide pharmacokinetics in patients.

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“…A pivotal role of plants in the health scenario is attributed to bioactive compounds, which could delay or inhibit the inception of degenerative diseases and increase life expectancy (Jagadish et al 2009). Plant-based products exhibit anticancer potential by apoptosis induction, anti-angiogensis, accumulation of p53, suppression of NF-kB activation, cell-cycle arrest, topoisomerase inhibition, preventing the oxidation of DNA, triggering carcinogen-detoxifying system, hindering carcinogen activation (Chainy et al 2000;Ren et al 2003) and many other pathways. Hence, it is imperative to explore novel natural sources for their anticancer efficacy.…”

Section: Introductionmentioning

confidence: 99%

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

Ashraf

Sarfraz

Rashid

et al. 2016

Pharmaceutical Biology

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Context Psidium guajava L. (Myrtaceae) leaves are used in traditional medicines for the treatment of cancer, inflammation and other ailments. Objective The current study explores scientific validation for this traditional medication. Materials and methods We used ferric-reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryl hydrazil (DPPH) assays to estimate antioxidant activity of P. guajava leaf extracts (methanol, hexane and chloroform). Antitumour and in vivo cytotoxic activities were determined using potato disc assay (PDA) and brine shrimp lethality assay, respectively. Three human carcinoma cell lines (KBM5, SCC4 and U266) were incubated with different doses (10-100 mg/mL) of extracts and the anticancer activity was estimated by MTT assay. NF-kB suppressing activity was determined using electrophoretic mobility shift assay (EMSA). Chemical composition of the three extracts was identified by GC-MS. Total phenolic and flavonoid contents were measured by colorimetric assays. Results and discussions The order of antioxidant activity of three extracts was methanol4chloro-form4hexane. The IC 50 values ranged from 22.73 to 51.65 mg/mL for KBM5; 22.82 to 70.25 mg/mL for SCC4 and 20.97 to 89.55 mg/mL for U266 cells. The hexane extract exhibited potent antitumour (IC 50 value ¼ 65.02 mg/mL) and cytotoxic (LC 50 value ¼ 32.18 mg/mL) activities. This extract also completely inhibited the TNF-a induced NF-kB activation in KBM5 cells. GC-MS results showed that pyrogallol, palmitic acid and vitamin E were the major components of methanol, chloroform and hexane extracts. We observed significant (p50.05) difference in total phenolic and flavonoid contents of different solvent extracts. Conclusion The present study demonstrates that P. guajava leaf extracts play a substantial role against cancer and down-modulate inflammatory nuclear factor kB. ARTICLE HISTORY

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Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro- lide

Cited by 9 publications

References 23 publications

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis

Tripterygium wilfordii bioactive compounds as anticancer and anti‐inflammatory agents

Chemical composition, antioxidant, antitumor, anticancer and cytotoxic effects ofPsidium guajavaleaf extracts

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