Induction of Mitochondrial-Dependent Apoptosis by an Essential Oil from Tanacetum gracile

Abstract

The essential oil of Tanacetum gracile (Accession no. AT-01 termed AT-01 in the manuscript), a cold desert alpine highly aromatic herb, has 40 constituents including lavendulol (21.5 %), lavendulol acetate (1.7 %), alpha-pinene (11.2 %), 1,8-cineole (15.2 %), CIS-beta-ocimene (6.9 %), borneol (6.1 %), limonene (5.1 %) and chamazulene (3.7 %). AT-01 was evaluated for its anticancer activity. It inhibited HL-60 cell proliferation with an IC (50) of 27 microg/mL. Furthermore, AT-01 induced apoptosis in human leukemia HL-60 cells as measured by several biological end points. AT-01 induced apoptotic body formation, enhanced annexinV-FITC binding of the cells, increased sub-G (0) DNA fraction, loss of mitochondrial membrane potential (Deltapsi (mt)) and release of cytochrome c from mitochondria, activated caspase-9 as well as caspase-3, and increased cleavage of PARP in HL-60 cells. Thus, AT-01 induced apoptosis through the mitochondrial dependent pathway in HL-60 cells.

Induction of reactive oxygen species–mediated apoptosis by purified Schisandrae semen essential oil in human leukemia U937 cells through activation of the caspase cascades and nuclear relocation of mitochondrial apoptogenic factors

“…The yield of the oil based on the dried weight of S. semen was 0.66%. were analyzed using a fluorescence microscope (Carl Zeiss, Oberkochen, Germany) [29]. To examine the involvement of caspases in apoptosis, the tripeptide pan-caspase inhibitor z-Val-Ala-Asp-fluoromethyl ketone (z-VAD-fmk, Calbiochem, San Diego, CA, USA) was added 2 h before treatment with SSeo.…”

“…Among plant-derived natural products, essential oils, which are concentrated hydrophobic liquids containing volatile aroma compounds from plants, have recently drawn scientific interest for their medicinal applications [19][20][21][22]. Essential oils are endowed with various pharmacological effects, including antibacterial [23], antioxidant [24], antifungal [25] and antimutagenic [26], and their assorted therapeutic potential has attracted the attention of many researchers to explore their anti-cancer activities [27][28][29][30].…”

Assessment of microtubule depolymerization property of flavonoids isolated from Tanacetum gracile in breast cancer cells by biochemical and molecular docking approach

“…The major class of compounds reported from the genus Tanacetum includes essential oils [4,5], sesquiterpene lactones, triterpenes [6][7][8] and flavonoids [9]. However, essential oils constitute a large number of monoterpenes and few phenolic compounds possess anticarcinogenic properties [10,11]. In our continuous search for promising cytotoxic agents from Indian medicinal plants, we investigated T. gracile by bioassay guided fractionation method [12].…”

Apoptotic Cell Death of Human Leukemia U937 Cells by Essential Oil purified from Schisandrae Semen

“…특히 다양한 식물 유래 천연물 중, 휘발성 아로마 화합물(volatile aroma compound)인 소수성 정유(essential oil)의 활용에 관한 연구가 최근 많은 관심을 끌고 있다 [2,8,30]. 그동안 많은 연구자들에 의하여 정유의 항균 [10,23], 항산화 [5], 항돌연변 이성 [24] 등의 효능을 바탕으로 항암활성에 관한 연구가 지속 적으로 이루어져 왔다 [18,20,27,28] 한편 IAP family에 속하는 단백질들은 caspase와 직, 간접 적으로 결합함으로서 caspase의 활성을 억제하여 apoptosis를 억제하는 것으로 알려져 있다 [12,26]. 따라서 SSeo가 IAP family 인자들의 발현에 어떠한 영향을 미치는 지를 확인한 결과, 조사된 대부분의 IAP family 단백질의 발현이 SSeo 처리 농도 의존적으로 감소되었다 (Fig.…”