Induction of MicroRNA-1 by Myocardin in Smooth Muscle Cells Inhibits Cell Proliferation

Chen, Jie; Yin, Hao; Jiang, Yulan; Radhakrishnan, Sarvan Kumar; Huang, Zhan-Peng; Li, Jingjing; Shi, Zhan; Kilsdonk, Elisabeth P.C.; Gui, Yu; Wang, Dazhi; Zheng, Xi-Long

doi:10.1161/atvbaha.110.218149

Cited by 125 publications

(99 citation statements)

References 38 publications

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“…reminiscent of the physiological spindle-like shape of contractile and quiescent VSMC in the tunica media of mature healthy blood vessels (Villaschi et al 1994). In some in vitro studies, elongated and spindle-like shape of the VSMC was also associated with lower proliferation activity and a higher differentiation status of these cells (Chen et al 2011). Another factor supporting the phenotypic Fig.…”

Section: Phenotypic Maturation Of Vsmc On Cellulosebased Materialsmentioning

confidence: 82%

Cellulose-based materials as scaffolds for tissue engineering

et al. 2013

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Two types of cellulose-based materials, 6-carboxycellulose with 2.1 or 6.6 wt% of -COOH groups, were prepared and tested for potential use in tissue engineering. The materials were functionalized with arginine, i.e. an amino acid with a basic side chain, or with chitosan, in order to balance the relatively acid character of oxidized cellulose molecules, and were seeded with vascular smooth muscle cells (VSMC). The cell adhesion and growth were then evaluated directly on the materials, and also on the underlying polystyrene culture dishes. Of these two types of studied materials, 6-carboxycellulose with 2.1 wt% of -COOH groups was more appropriate for cell colonization. The cells on this material achieved an elongated shape, while they were spherical in shape on the other materials. The number of cells and the concentration (per mg of protein) of contractile proteins alpha-actin and SM1 and SM2 myosins, i.e. markers of the phenotypic maturation of VSMC, were also significantly higher on this material. Functionalization of the material with arginine and chitosan further improved the phenotypic maturation of VSMC. Chitosan also improved the adhesion and growth of these cells. In comparison with the control polystyrene dishes, the proliferation of cells on our cellulose-based materials was relatively low. This suggests that these materials can be used in applications where high proliferation activity of cells is not desirable, e.g. proliferation of VSMC on vascular prostheses. Alternatively, the cell proliferation might be enhanced by another more efficient modification, which would require further research.

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Section: Phenotypic Maturation Of Vsmc On Cellulosebased Materialsmentioning

confidence: 82%

Cellulose-based materials as scaffolds for tissue engineering

et al. 2013

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“…In vivo, miR-1 and miR-133 synergistically specify cardiomyocytes by suppressing myocardin during the development of heart (128)(129)(130). In cultured SMCs, miR-1 also suppressed SMC differentiation by targeting myocardin; however, the level of miR-1 in vSMCs in vivo is negligible (131,132). In contrast, miR-133 is expressed in vSMCs in vivo and is regulated by platelet-derived growth factor-induced activation of MAPK/ extracellular signal-regulated kinase 1/2.…”

Section: Mir-133 In Smc Differentiationmentioning

confidence: 99%

The Roles of MicroRNAs and Protein Components of the MicroRNA Pathway in Lung Development and Diseases

Cushing

Jiang

Kuang

et al. 2015

Am J Respir Cell Mol Biol

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Decades of studies have shown evolutionarily conserved molecular networks consisting of transcriptional factors, diffusing growth factors, and signaling pathways that regulate proper lung development. Recently, microRNAs (miRNAs), small, noncoding regulatory RNAs, have been integrated into these networks. Significant advances have been made in characterizing the developmental stage-or cell type-specific miRNAs during lung development by using approaches such as genome-wide profiling and in situ hybridization. Results from gain-or loss-of-function studies revealed pivotal roles of protein components of the miRNA pathway and individual miRNAs in regulating proliferation, apoptosis, differentiation, and morphogenesis during lung development. Aberrant expression or functions of these components have been associated with pulmonary disorders, suggesting their involvement in pathogenesis of these diseases. Moreover, genetically modified mice generated in these studies have become useful models of human lung diseases. Challenges in this field include characterization of collective function and responsible targets of miRNAs specifically expressed during lung development, and translation of these basic findings into clinically relevant information for better understanding of human diseases. The goal of this review is to discuss the recent progress on the understanding of how the miRNA pathway regulates lung development, how dysregulation of miRNA activities contributes to pathogenesis of related pulmonary diseases, and to identify relevant questions and future directions.

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“…miR-1 and miR-21 are two of numerous representatives of microRNAs. miR-1, initially found in cardiac muscle cells (12), but also found in various tissues (13)(14)(15), was strongly suspected of acting as a tumor suppressor due to its antioncogenic properties (16) in prostate, breast and colorectal cancer (13)(14)(15); interestingly, the type of tissue in fact determines the biological behavior of miR-1 (9). Sadly, the current data situation for the expression of miR-1 in RCC leaves much to be desired.…”

mentioning

confidence: 99%

MicroRNA-1 and MicroRNA-21 Individually Regulate Cellular Growth of Non-malignant and Malignant Renal Cells

Ahrend

Kaul

Ziegler

et al. 2017

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Abstract. Background/Aim: Due to its poor prognosis, it is increasingly necessary to understand the biology of renal cell cancer (RCC). Therefore, we investigated the role of microRNAs miR-1 and miR-21 in the growth of RCC cells compared to that of non-malignant renal cells. Materials and Methods: Four malignant cell lines RCC4, A498) Tumorigenesis gives cells an advantage in survival by an increase of proliferation, angiogenesis, immunomodulation and immortality (1-4). Due to its delayed diagnosis until advanced stages, renal cell cancer (RCC) is known as the deadliest neoplasm of the urinary tract (1, 5). Thus, it is more necessary than ever to understand its tumor biology and explore new targets for further therapeutic approaches and prognostic options (6). Former studies suggested that among others, a misguided microRNA expression pattern may be involved in tumor initiation and progression in RCC (7,8). Among such mechanisms, small regulatory RNAs, so-called microRNAs (miRs), can operate as controllers of different proccesses in tumorigenesis, such as cell migration and differentiation, as well as metastasis and apoptosis (9-11). miR-1 and miR-21 are two of numerous representatives of microRNAs. miR-1, initially found in cardiac muscle cells (12), but also found in various tissues (13-15), was strongly suspected of acting as a tumor suppressor due to its antioncogenic properties (16) in prostate, breast and colorectal cancer (13-15); interestingly, the type of tissue in fact determines the biological behavior of miR-1 (9). Sadly, the current data situation for the expression of miR-1 in RCC leaves much to be desired. For this reason, our study aimed to expand the first findings in order to gain a better understanding of the tumor biology of RCC. Similarly to miR-1, miR-21 was also detected in various malignant tissues, such as prostate and lung cancer and their metastases, pancreatic cancer and colorectal cancer (17-21). However, in contrast to miR-1, miR-21 appears to be an oncomir. There are preliminary investigations, showing miR-21 to appear as an oncomir in the case of RCC (22, 23).The fact that cancer cells have a further survival advantage by extending their chromosomal ends by means of endogenic telomerase, not only raises a problem, but also provides a therapeutic approach in cancer therapy (4).In order to gain deeper insight into these issues, as far as we are aware, we are the first to investigate one non-malignant 625 This article is freely accessible online. *These Authors contributed equally to this study. Materials and MethodsCell culture. Human RCC cell lines Caki-1, 786-O, A498 (all Cell Lines Service, Eppelheim, Germany), and RCC4 (Sigma-Aldrich, München, Germany) and the non-malignant renal cell line RC-124 (Cell Lines Service) were applied in this study. Caki-1 and A498 cells were propagated in minimum essential medium (MEM) supplemented with 79.6 mg/l non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 1% penicillline/streptomycin (P/S), and 10% fetal bovine serum (FBS...

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Induction of MicroRNA-1 by Myocardin in Smooth Muscle Cells Inhibits Cell Proliferation

Cited by 125 publications

References 38 publications

Cellulose-based materials as scaffolds for tissue engineering

Cellulose-based materials as scaffolds for tissue engineering

The Roles of MicroRNAs and Protein Components of the MicroRNA Pathway in Lung Development and Diseases

MicroRNA-1 and MicroRNA-21 Individually Regulate Cellular Growth of Non-malignant and Malignant Renal Cells

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