Induction of micronuclei in the domestic cat (Felis domesticus) peripheral blood by colchicine and cytosine-arabinoside

Zúñiga‐González, Guillermo Moisés; Ramı́rez-Muñoz, M.P; Torres-Bugarı́n, Olivia; Pérez‐Jiménez, Jara; Ramos-Mora, Alberto; Zamora‐Pérez, Ana Lourdes; Gallegos‐Arreola, Martha Patricia; Sánchez‐Corona, José

doi:10.1016/s1383-5718(97)00184-8

Cited by 13 publications

(13 citation statements)

References 10 publications

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“…In fact, they are in direct contact with the surrounding water, while the germinating cells of the skin are covered by a thin stratum of epidermal cells; nonetheless, differences in cellular turnover, activity, metabolism or DNA repair should not excluded. This work confirms a higher sensitivity of the MN test when applied on younger individuals [National Cancer Institute, ] as observed in mice [Sanderson and Clark, ], rats [Udroiu et al, ], cats [Zúñiga‐González et al, ], pigs [Martinez, ], and humans [Neri et al, ]. We speculate that behind the different MN frequencies observed between adult and larval samples there could be both a difference in tissue‐specific sensitivity and the diverse life habit characterizing the postmetamorph and larval animals, the former being aquatic only during the reproduction period [Vignoli et al, ], whilst the latter being aquatic for the whole life‐stage.…”

Section: Discussionsupporting

confidence: 84%

Micronucleus test on Triturus carnifex as a tool for environmental biomonitoring

Udroiu

Sgura

Vignoli

et al. 2014

Environ and Mol Mutagen

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The amphibian micronucleus test has been widely used during the last 30 years to test the genotoxic properties of several chemicals and as a tool for ecogenotoxic monitoring. The vast majority of these studies were performed on peripheral blood of urodelan larvae and anuran tadpoles and to a lesser extent adults were also used. In this study, we developed protocols for measuring micronuclei in adult shed skin cells and larval gill cells of the Italian crested newt (Triturus carnifex). Amphibians were collected from ponds in two protected areas in Italy that differed in their radon content. Twenty-three adult newts and 31 larvae were captured from the radon-rich pond, while 20 adults and 27 larvae were taken from the radon-free site. The animals were brought to the laboratory and the micronucleus test was performed on peripheral blood and shed skins taken from the adults and on larval gills. Samples from the radon-rich site showed micronucleus frequencies higher than those from the radon-free site and the difference was statistically significant in gill cells (P < 0.00001). Moreover, the larval gills seem to be more sensitive than the adult tissues. This method represents an easy (and noninvasive in the case of the shed skin) application of the micronucleus assay that can be useful for environmental studies in situ.

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Section: Discussionsupporting

confidence: 84%

Micronucleus test on Triturus carnifex as a tool for environmental biomonitoring

Udroiu

Sgura

Vignoli

et al. 2014

Environ and Mol Mutagen

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“…These compounds are known to pass into breast milk [10, 24], although the transference of COL through breast milk is undefined. The following groups were formed (five rats per group), each containing a mother and five of her offspring: Group 1 (negative control), which was given sterile water; Groups 2, 3, and 4, which were given CP at 5, 10, or 20 mg/kg/day [17]; Groups 5, 6, and 7, which were given COL at 0.1, 0.2, or 0.4 mg/kg/day [25]; and Groups 8, 9, and 10, which were given AraC at 3, 4.5, or 6 mg/kg/day [26, 27], respectively. All doses administered by oral gavage were adjusted to a final volume of 0.5 mL with sterile water, and the doses administered by intramuscular administration were adjusted to a final volume of 0.2 mL with sterile water.…”

Section: Methodsmentioning

confidence: 99%

Micronucleated Erythrocytes in Peripheral Blood from Neonate Rats Exposed by Breastfeeding to Cyclophosphamide, Colchicine, or Cytosine-Arabinoside

Gómez‐Meda

Bañales-Martínez

Zamora‐Pérez

et al. 2016

BioMed Research International

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Genotoxic exposure to chemical substances is common, and nursing mothers could transmit harmful substances or their metabolites to their offspring through breast milk. We explored the possibility of determining genotoxic effects in the erythrocytes of breastfeeding rat pups whose mothers received a genotoxic compound while nursing. Ten groups of female rats and five pups per dam were studied. The control group received sterile water, and the experimental groups received one of three different doses of cyclophosphamide, colchicine, or cytosine-arabinoside. Blood smears were prepared from samples taken from each dam and pup every 24 h for six days. There were increased numbers of micronucleated erythrocytes (MNEs) and micronucleated polychromatic erythrocytes (MNPCEs) in the samples from pups in the experimental groups (P < 0.02) and increased MNPCE frequencies in the samples from the dams (P < 0.05). These results demonstrate the vertical transmission of the genotoxic effect of the compounds tested. In conclusion, assessing MNEs in breastfeeding neonate rats to assess DNA damage may be a useful approach for identifying genotoxic compounds and/or cytotoxic effects. This strategy could help in screening for therapeutic approaches that are genotoxic during the lactation stage and these assessments might also be helpful for developing preventive strategies to counteract harmful effects.

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“…Since 1996, our group has worked on identifying animal species with measurable frequencies of spontaneous micronucleated erythrocytes (MNEs) circulating in their peripheral blood [Zúñiga et al, 1996a;Zúñiga-González et al, 2000;Zúñiga-González et al, 2001a;Zúñiga-González et al, 2002], with the idea that analysis of MNE frequencies in these animals can be used as a biomonitor of exposure to genotoxic agents [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b;Gómez-Meda et al, 2004]. A highly proliferating tissue like blood is required to carry out this assay [Lähdetie, 1983;Shuilin and Baker, 1989;Schmezer et al, 1990;Torres-Bugarín et al, 1998;Zamora-Perez et al, 2004], and only a drop of blood is needed to measure micronucleus (MN) frequencies in peripheral blood, so that it is not necessary to kill the animal [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b].…”

Section: Introductionmentioning

confidence: 99%

“…A highly proliferating tissue like blood is required to carry out this assay [Lähdetie, 1983;Shuilin and Baker, 1989;Schmezer et al, 1990;Torres-Bugarín et al, 1998;Zamora-Perez et al, 2004], and only a drop of blood is needed to measure micronucleus (MN) frequencies in peripheral blood, so that it is not necessary to kill the animal [Zúñiga-González et al, 1998;Zúñiga-González et al, 2001b]. In some species, spontaneous MNEs can be observed in peripheral blood [Zúñiga et al, 1996a;Zúñiga et al, 2000;Zúñiga-González et al, 2001a], because the reticuloendothelial system inefficiently removes them from the circulation.…”

Section: Introductionmentioning

confidence: 99%

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

Zúñiga‐González

Gómez‐Meda

Zamora‐Pérez

et al. 2005

Environ. Mol. Mutagen.

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Nonhuman primates are of particular relevance in evaluating the potential toxicity of drugs and environmental agents. We have used previously published information and data from the present study to establish a relationship for New World (NW) and Old World (OW) primates on the basis of the frequency of spontaneous micronucleated erythrocytes (MNEs) observed in peripheral blood. Data on spontaneous MNEs in peripheral blood from 15 species of primates, including humans, indicate that NW primates have significantly (P < 0.01) higher MNE frequencies (group mean, 9.5 +/- 7.3 MNEs/10,000 erythrocytes; range, 0.7-20.5/10,000 erythrocytes) than OW primates (group mean, 1.0 +/- 0.9 MNEs/10,000 erythrocytes; range, 0.0-2.6 MNEs/10,000 erythrocytes). Humans are believed to have developed in the OW, and human MNE frequencies were similar to those described for OW primate species. We selected the common marmoset (Callithrix jacchus), a NW primate, to determine whether therapeutic pediatric doses of Metotrexate (MTX; 2.5 mg/kg), Cyclophosphamide (CP; 5 mg/kg), Cytosine-arabinoside (Ara-C; 3 mg/kg), or 5-Fluorouracil (5-FU; 10 mg/kg), administered daily for two consecutive days, increase the frequency of micronuclei. Micronucleated polychromatic erythrocyte frequencies were increased significantly in groups receiving MTX, CP and Ara-C, while MNE frequencies were increased by the Ara-C treatment. The results of this study indicate that NW primates have higher spontaneous MNE frequencies than OW primates, and because of this, NW primates like the common marmoset, may be suitable for evaluating the genotoxicity of chemical agents.

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Induction of micronuclei in the domestic cat (Felis domesticus) peripheral blood by colchicine and cytosine-arabinoside

Cited by 13 publications

References 10 publications

Micronucleus test on Triturus carnifex as a tool for environmental biomonitoring

Micronucleus test on Triturus carnifex as a tool for environmental biomonitoring

Micronucleated Erythrocytes in Peripheral Blood from Neonate Rats Exposed by Breastfeeding to Cyclophosphamide, Colchicine, or Cytosine-Arabinoside

Micronucleated erythrocyte frequencies in old and new world primates: Measurement of micronucleated erythrocyte frequencies in peripheral blood ofCallithrix jacchus as a model for evaluating genotoxicity in primates

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