Induction of Micronuclei in Eel (Anguilla anguilla L.) by Heavy Metals

Sanchez-Galan, S.; Linde, A. R.; Ayllón, Fernando; García‐Vázquez, Eva

doi:10.1006/eesa.2001.2048

Cited by 63 publications

(24 citation statements)

References 17 publications

(20 reference statements)

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“…b After 96 h exposure [the best-fit logarithmic equations for these data were y= 9.331+1.219 ln x (r 2 =0.606) for Cu, y=3.298+0.414 ln x (r 2 =0.965) for Pb, and y=5.165+0.538 ln x (r 2 =0.933) for As] mercury, only to cadmium. Analogous results were also observed in the eel Anguilla anguilla (Sanchéz-Galán et al 2001;Gravato et al 2006). In this same species exposed during 7 days to 0.2 μM Cu, Oliveira et al (2008) did not observed significant changes in the DNA integrity (measured by DNA alkaline unwinding assay) in the blood, gill, liver, and kidney, but a significant increase in erythrocyte nuclear abnormalities (ENA) frequency was observed.…”

Section: Discussionsupporting

confidence: 64%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

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Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

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Section: Discussionsupporting

confidence: 64%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

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“…Induction of micronuclei was reported previously for fish exposed to Cd [Ayllon and Garcia Vazquez, 2000;Sanchez-Galan et al, 2001;Zhu et al, 2004;Arkhipchuk and Garanko, 2005;Cavas et al, 2005], Cu [Zhu et al, 2004;Arkhipchuk and Garanko, 2005;Cavas et al, 2005], Cr [Al-Sabti et al, 1994;de Lemos et al, 2001;Zhu et al, 2004], and Pb Ferraro et al, 2004]. Therefore, it is plausible that elevated concentrations of heavy metals could have contributed to the elevated MN and NA frequencies produced by the downstream Berdan River water samples.…”

Section: Discussionmentioning

confidence: 76%

Evaluation of river water genotoxicity using the piscine micronucleus test

Ergene

Çavaş

Çelık

et al. 2007

Environ and Mol Mutagen

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The Berdan River, which empties into the Mediterranean Sea on the east coast of Turkey, receives discharges of industrial and municipal waste. In the present study, the in vivo piscine micronucleus (MN) test was used to evaluate the genotoxicity of water samples collected from different locations along the Berdan River. Nile tilapia (Oreochromis niloticus) were exposed in the laboratory for 2, 4, and 6 days, and micronuclei were evaluated in peripheral blood erythrocytes, gill cells, and caudal fin epithelial cells. A single dose of 5 mg/L cyclophosphamide was used as a positive control. In addition to micronuclei, nuclear abnormalities (NAs), such as binucleated cells and blebbed, notched, and lobed nuclei, were assessed in the erythrocytes, and chemical analyses were carried out to determine the amount of heavy metals in the water samples. MN and NA frequencies were significantly elevated (up to 2- to 3-fold) in fish exposed to river water samples taken downstream of potential discharges, and the elevated responses in gill and fin cells were related to the concentration of heavy metals in the water. MN frequencies (expressed as micronucleated cells/1,000 cells), in both treated and untreated fish, were greatest in gill cells (range: 0.80-3.70), and generally lower in erythrocytes (range: 0.50-2.80), and fin cells (range: 0.45-1.70). The results of this study indicate that the Berdan River is contaminated with genotoxic pollutants and that the genotoxicity is related to the discharge of wastes into the river water.

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“…The comet assay has been used as a sensitive tool to measure DNA damage in a variety of organisms [8][9][10], while the micronucleus test, a cytogenetic technique, has been used to detect chromosomal aberrations and nuclear abnormalities such as binuclei and micronuclei-these are well-established indicators of cytotoxicity and genetic toxicology, respectively [11,12].…”

Section: Introductionmentioning

confidence: 99%

Comparative Genotoxicity of Cadmium and Lead in Earthworm Coelomocytes

Muangphra

Gooneratne

2011

Applied and Environmental Soil Science

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To determine genotoxicity to coelomocytes, Pheretima peguana earthworms were exposed in filter paper studies to cadmium (Cd) and lead (Pb) for 48 h, at concentrations less than the LC 10 -Cd: 0.09, 0.19, 0.38, 0.75, and 1.50 µg cm −2 ; Pb: 1.65, 3.29, 6.58, 13.16, and 26.32 µg cm −2 . For Cd at 0.75 µg cm −2 , in the micronucleus test (detects chromosomal aberrations), significant increases (P < .05) in micronuclei and binucleate cells were observed, and in the comet assay (detects DNA single-strand breaks), tail DNA% was significantly increased. Lead was less toxic with minimal effects on DNA, but the binucleates were significantly increased by Pb at 3.29 µg cm −2 . This study shows that Cd is more acutely toxic and sublethally genotoxic than Pb to P. peguana. Cadmium caused chromosomal aberrations and DNA single-strand breaks at 45% of the LC 10 concentration. Lead, in contrast, did not induce DNA damage but caused cytokinesis defects.

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Induction of Micronuclei in Eel (Anguilla anguilla L.) by Heavy Metals

Cited by 63 publications

References 17 publications

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Evaluation of river water genotoxicity using the piscine micronucleus test

Comparative Genotoxicity of Cadmium and Lead in Earthworm Coelomocytes

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